Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat Models
Ionic self-assembly of the peptide RADARADARADARADA (RADA16-1) may form a well-defined nanofiber and eventually a hydrogel scaffold, with a water content of over 99.5%. This leads to the establishment of a nanofiber barrier that can be used to achieve complete hemostasis in less than 20 s in multipl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2012-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/13/11/15279 |
| _version_ | 1811202175342215168 |
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| author | Fei Lv Xiaojun Zhao Songtao Wang Xiaozhong Zhong Ting Wang |
| author_facet | Fei Lv Xiaojun Zhao Songtao Wang Xiaozhong Zhong Ting Wang |
| author_sort | Fei Lv |
| collection | DOAJ |
| description | Ionic self-assembly of the peptide RADARADARADARADA (RADA16-1) may form a well-defined nanofiber and eventually a hydrogel scaffold, with a water content of over 99.5%. This leads to the establishment of a nanofiber barrier that can be used to achieve complete hemostasis in less than 20 s in multiple tissues and in a variety of different wounds. In the present study, the nanofiber scaffolds of RADA16-1 peptide were sonicated into smaller fragments to identify possible molecular mechanisms underlying the rapid cessation of bleeding associated with these materials. Atomic force microscopy (AFM), circular dichroism (CD), and rheometry were also used to evaluate the re-assembly kinetics of this peptide. A bleeding control experiment was performed in animal models to uncover the molecular mechanisms underlying this fast hemostasis. In this way, these sonicated fragments not only quickly reassembled into nanofibers indistinguishable from the original material, but the degree of reassembly was also correlated with an increase in the rigidity of the scaffold and increased as the time required for hemostasis increased. |
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| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T02:34:25Z |
| publishDate | 2012-11-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-90d1665a449549988c8cc39e03a91cf72022-12-22T03:51:37ZengMDPI AGInternational Journal of Molecular Sciences1422-00672012-11-011311152791529010.3390/ijms131115279Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat ModelsFei LvXiaojun ZhaoSongtao WangXiaozhong ZhongTing WangIonic self-assembly of the peptide RADARADARADARADA (RADA16-1) may form a well-defined nanofiber and eventually a hydrogel scaffold, with a water content of over 99.5%. This leads to the establishment of a nanofiber barrier that can be used to achieve complete hemostasis in less than 20 s in multiple tissues and in a variety of different wounds. In the present study, the nanofiber scaffolds of RADA16-1 peptide were sonicated into smaller fragments to identify possible molecular mechanisms underlying the rapid cessation of bleeding associated with these materials. Atomic force microscopy (AFM), circular dichroism (CD), and rheometry were also used to evaluate the re-assembly kinetics of this peptide. A bleeding control experiment was performed in animal models to uncover the molecular mechanisms underlying this fast hemostasis. In this way, these sonicated fragments not only quickly reassembled into nanofibers indistinguishable from the original material, but the degree of reassembly was also correlated with an increase in the rigidity of the scaffold and increased as the time required for hemostasis increased.http://www.mdpi.com/1422-0067/13/11/15279RADA16-1nanofiber scaffoldsself-assemblyhemostasis |
| spellingShingle | Fei Lv Xiaojun Zhao Songtao Wang Xiaozhong Zhong Ting Wang Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat Models International Journal of Molecular Sciences RADA16-1 nanofiber scaffolds self-assembly hemostasis |
| title | Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat Models |
| title_full | Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat Models |
| title_fullStr | Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat Models |
| title_full_unstemmed | Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat Models |
| title_short | Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat Models |
| title_sort | molecular mechanisms of rada16 1 peptide on fast stop bleeding in rat models |
| topic | RADA16-1 nanofiber scaffolds self-assembly hemostasis |
| url | http://www.mdpi.com/1422-0067/13/11/15279 |
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