Evaluation of Recombinant Bovine Interleukin-8 (rbIL-8) as a Treatment for Chronic Intramammary Infection in Dairy Cows
Mastitis is one of the main contributors to antimicrobial resistance in livestock, so alternative therapies are being investigated to address it. The present study assessed the capability of recombinant bovine interleukin-8 (rbIL-8) to improve neutrophil function in the mammary gland and resolve chr...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/11/8/1029 |
| _version_ | 1797432579606970368 |
|---|---|
| author | Phillip M. G. Peixoto Lais L. Cunha Leonardo Barbosa Wilson Coelho Giorgia Podico Rodrigo C. Bicalho Igor F. Canisso Fabio S. Lima |
| author_facet | Phillip M. G. Peixoto Lais L. Cunha Leonardo Barbosa Wilson Coelho Giorgia Podico Rodrigo C. Bicalho Igor F. Canisso Fabio S. Lima |
| author_sort | Phillip M. G. Peixoto |
| collection | DOAJ |
| description | Mastitis is one of the main contributors to antimicrobial resistance in livestock, so alternative therapies are being investigated to address it. The present study assessed the capability of recombinant bovine interleukin-8 (rbIL-8) to improve neutrophil function in the mammary gland and resolve chronic high somatic cell count (SCC) in Holstein cows. Multiparous cows (n = 8) with more than 300,000 SCC per mL were allocated to one of two intramammary infusions: saline (10 mL of saline solution) or rbIL-8 (1.57 mg/mL of recombinant bovine IL-8 diluted in 9 mL of saline). In addition, there was an untreated control group (n = 2, SCC < 300,000 SCC/mL). Milk samples were collected post-treatment at 0, 4, 8, 12, 24, 48, and 144 h to quantify milk SCC, haptoglobin, and IgG concentrations. Neutrophil’s phagocytosis in milk and blood was evaluated via flow cytometry at 0, 24, and 48 h. The log of SCC did not differ between the infused groups (<i>p</i> = 0.369). Neutrophils presented a similar log of cells with high fluorescence for propidium-iodide (PI) and dihydrorhodamine (DHR) in milk (<i>p</i> = 0.412) and blood samples (<i>p</i> = 0.766) in both infused groups. Intramammary infusion of 1.57 mg/mL of rbIL-8 did not improve neutrophils response and failed to resolve chronic high SCC. |
| first_indexed | 2024-03-09T10:02:39Z |
| format | Article |
| id | doaj.art-90d5a0e775d544909665f4ec1019259d |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T10:02:39Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-90d5a0e775d544909665f4ec1019259d2023-12-01T23:19:27ZengMDPI AGAntibiotics2079-63822022-07-01118102910.3390/antibiotics11081029Evaluation of Recombinant Bovine Interleukin-8 (rbIL-8) as a Treatment for Chronic Intramammary Infection in Dairy CowsPhillip M. G. Peixoto0Lais L. Cunha1Leonardo Barbosa2Wilson Coelho3Giorgia Podico4Rodrigo C. Bicalho5Igor F. Canisso6Fabio S. Lima7Department of Veterinary Clinical Medicine, University of Illinois, Urbana, IL 61802, USADepartment of Veterinary Clinical Medicine, University of Illinois, Urbana, IL 61802, USADepartment of Veterinary Clinical Medicine, University of Illinois, Urbana, IL 61802, USADepartment of Veterinary Clinical Medicine, University of Illinois, Urbana, IL 61802, USADepartment of Veterinary Clinical Medicine, University of Illinois, Urbana, IL 61802, USAFera Diagnostics and Biologicals Corporation, College Station, TX 77802, USADepartment of Veterinary Clinical Medicine, University of Illinois, Urbana, IL 61802, USADepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USAMastitis is one of the main contributors to antimicrobial resistance in livestock, so alternative therapies are being investigated to address it. The present study assessed the capability of recombinant bovine interleukin-8 (rbIL-8) to improve neutrophil function in the mammary gland and resolve chronic high somatic cell count (SCC) in Holstein cows. Multiparous cows (n = 8) with more than 300,000 SCC per mL were allocated to one of two intramammary infusions: saline (10 mL of saline solution) or rbIL-8 (1.57 mg/mL of recombinant bovine IL-8 diluted in 9 mL of saline). In addition, there was an untreated control group (n = 2, SCC < 300,000 SCC/mL). Milk samples were collected post-treatment at 0, 4, 8, 12, 24, 48, and 144 h to quantify milk SCC, haptoglobin, and IgG concentrations. Neutrophil’s phagocytosis in milk and blood was evaluated via flow cytometry at 0, 24, and 48 h. The log of SCC did not differ between the infused groups (<i>p</i> = 0.369). Neutrophils presented a similar log of cells with high fluorescence for propidium-iodide (PI) and dihydrorhodamine (DHR) in milk (<i>p</i> = 0.412) and blood samples (<i>p</i> = 0.766) in both infused groups. Intramammary infusion of 1.57 mg/mL of rbIL-8 did not improve neutrophils response and failed to resolve chronic high SCC.https://www.mdpi.com/2079-6382/11/8/1029subclinical mastitisSCCinterleukin 8 |
| spellingShingle | Phillip M. G. Peixoto Lais L. Cunha Leonardo Barbosa Wilson Coelho Giorgia Podico Rodrigo C. Bicalho Igor F. Canisso Fabio S. Lima Evaluation of Recombinant Bovine Interleukin-8 (rbIL-8) as a Treatment for Chronic Intramammary Infection in Dairy Cows Antibiotics subclinical mastitis SCC interleukin 8 |
| title | Evaluation of Recombinant Bovine Interleukin-8 (rbIL-8) as a Treatment for Chronic Intramammary Infection in Dairy Cows |
| title_full | Evaluation of Recombinant Bovine Interleukin-8 (rbIL-8) as a Treatment for Chronic Intramammary Infection in Dairy Cows |
| title_fullStr | Evaluation of Recombinant Bovine Interleukin-8 (rbIL-8) as a Treatment for Chronic Intramammary Infection in Dairy Cows |
| title_full_unstemmed | Evaluation of Recombinant Bovine Interleukin-8 (rbIL-8) as a Treatment for Chronic Intramammary Infection in Dairy Cows |
| title_short | Evaluation of Recombinant Bovine Interleukin-8 (rbIL-8) as a Treatment for Chronic Intramammary Infection in Dairy Cows |
| title_sort | evaluation of recombinant bovine interleukin 8 rbil 8 as a treatment for chronic intramammary infection in dairy cows |
| topic | subclinical mastitis SCC interleukin 8 |
| url | https://www.mdpi.com/2079-6382/11/8/1029 |
| work_keys_str_mv | AT phillipmgpeixoto evaluationofrecombinantbovineinterleukin8rbil8asatreatmentforchronicintramammaryinfectionindairycows AT laislcunha evaluationofrecombinantbovineinterleukin8rbil8asatreatmentforchronicintramammaryinfectionindairycows AT leonardobarbosa evaluationofrecombinantbovineinterleukin8rbil8asatreatmentforchronicintramammaryinfectionindairycows AT wilsoncoelho evaluationofrecombinantbovineinterleukin8rbil8asatreatmentforchronicintramammaryinfectionindairycows AT giorgiapodico evaluationofrecombinantbovineinterleukin8rbil8asatreatmentforchronicintramammaryinfectionindairycows AT rodrigocbicalho evaluationofrecombinantbovineinterleukin8rbil8asatreatmentforchronicintramammaryinfectionindairycows AT igorfcanisso evaluationofrecombinantbovineinterleukin8rbil8asatreatmentforchronicintramammaryinfectionindairycows AT fabioslima evaluationofrecombinantbovineinterleukin8rbil8asatreatmentforchronicintramammaryinfectionindairycows |