Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49–50 exons in the DMD gene
Duchenne muscular dystrophy (DMD), an X-linked genetic disorder characterized by progressive muscle weakness and atrophies affecting skeletal and cardiac muscles, is caused by mutations in dystrophin (DMD) gene that spans 79 exons. Here, we generated iPSCs from a Chinese patient with 49–50 exons del...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350611930296X |
| _version_ | 1818996896171032576 |
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| author | Jingyun Guan Xinnong Liu Haiyan Zhang Xiaomeng Yang Yanyan Ma Yue Li Zhongtao Gai Yi Liu |
| author_facet | Jingyun Guan Xinnong Liu Haiyan Zhang Xiaomeng Yang Yanyan Ma Yue Li Zhongtao Gai Yi Liu |
| author_sort | Jingyun Guan |
| collection | DOAJ |
| description | Duchenne muscular dystrophy (DMD), an X-linked genetic disorder characterized by progressive muscle weakness and atrophies affecting skeletal and cardiac muscles, is caused by mutations in dystrophin (DMD) gene that spans 79 exons. Here, we generated iPSCs from a Chinese patient with 49–50 exons deletion in DMD gene by reprogramming peripheral blood mononuclear cells with non-integrating vectors. The generated iPSCs line (SDQLCHi007-A) carrying the identical deletion of 49–50 exons, expresses pluripotency markers, presents a normal karyotype and is able to differentiate into three germ layers. Keywords: DMD gene, iPSCs, PBMC |
| first_indexed | 2024-12-20T21:37:01Z |
| format | Article |
| id | doaj.art-90d7a772257949cb9555d898f38e6120 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-20T21:37:01Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-90d7a772257949cb9555d898f38e61202022-12-21T19:25:54ZengElsevierStem Cell Research1873-50612020-01-0142Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49–50 exons in the DMD geneJingyun Guan0Xinnong Liu1Haiyan Zhang2Xiaomeng Yang3Yanyan Ma4Yue Li5Zhongtao Gai6Yi Liu7Pediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaDepartment of Vascular Surgery, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037,ChinaPediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaPediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaPediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaPediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaPediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, China; Corresponding authors.Pediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, China; Corresponding authors.Duchenne muscular dystrophy (DMD), an X-linked genetic disorder characterized by progressive muscle weakness and atrophies affecting skeletal and cardiac muscles, is caused by mutations in dystrophin (DMD) gene that spans 79 exons. Here, we generated iPSCs from a Chinese patient with 49–50 exons deletion in DMD gene by reprogramming peripheral blood mononuclear cells with non-integrating vectors. The generated iPSCs line (SDQLCHi007-A) carrying the identical deletion of 49–50 exons, expresses pluripotency markers, presents a normal karyotype and is able to differentiate into three germ layers. Keywords: DMD gene, iPSCs, PBMChttp://www.sciencedirect.com/science/article/pii/S187350611930296X |
| spellingShingle | Jingyun Guan Xinnong Liu Haiyan Zhang Xiaomeng Yang Yanyan Ma Yue Li Zhongtao Gai Yi Liu Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49–50 exons in the DMD gene Stem Cell Research |
| title | Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49–50 exons in the DMD gene |
| title_full | Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49–50 exons in the DMD gene |
| title_fullStr | Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49–50 exons in the DMD gene |
| title_full_unstemmed | Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49–50 exons in the DMD gene |
| title_short | Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49–50 exons in the DMD gene |
| title_sort | reprogramming of human peripheral blood mononuclear cell pbmc from a chinese patient suffering duchenne muscular dystrophy to ipsc line sdqlchi007 a carrying deletion of 49 50 exons in the dmd gene |
| url | http://www.sciencedirect.com/science/article/pii/S187350611930296X |
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