Therapeutic potential of natural products and antibiotics against bovine mastitis pathogen of cows and buffaloes
The present study aims to evaluate the prevalence and antimicrobial sensitivity of Staphylococcus aureus associated with bovine mastitis to selected antibiotics and plant extracts. In the current study, 140 milk samples were collected from cows and buffaloes. Among the 140 samples, 93 samples were p...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2023-07-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-202307-0002_therapeutic-potential-of-natural-products-and-antibiotics-against-bovine-mastitis-pathogen-of-cows-and-buffaloe.php |
| _version_ | 1827890748046966784 |
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| author | W Khan SA Khan FA Khan S Khan I Ullah A Shah I Uddin S Khan H Khan MN Uddin F Akbar N Qayum |
| author_facet | W Khan SA Khan FA Khan S Khan I Ullah A Shah I Uddin S Khan H Khan MN Uddin F Akbar N Qayum |
| author_sort | W Khan |
| collection | DOAJ |
| description | The present study aims to evaluate the prevalence and antimicrobial sensitivity of Staphylococcus aureus associated with bovine mastitis to selected antibiotics and plant extracts. In the current study, 140 milk samples were collected from cows and buffaloes. Among the 140 samples, 93 samples were positive for sub-clinical mastitis based on the California Mastitis Test (CMT). Out of the total positive samples, 45 were confirmed for S. aureus on a Mannitol salt agar media. The antimicrobial susceptibility test revealed that 44.82% of the isolates were resistant to cefoxitin (oxacillin) confirming methicillin-resistant S. aureus (MRSA) with a higher percentage (51.61%) in the buffalo than in the cow samples. Furthermore, the PCR assay confirmed the presence of the mecA gene in all the MRSA isolates. Among the seven tested antibiotics, sulfamethoxazole + trimethoprim showed high efficacy (71.1%) against methicillin-susceptible S. aureus isolates (MSSA). Oxytetracycline and sulfamethoxazole + trimethoprim showed 20% efficacy against MRSA followed by enrofloxacin (10%). On the other hand, the tested samples from Pistacia chinensis revealed that the ethyl acetate extract of bark showed a maximum zone of inhibition of 21.3 mm against MSSA and MRSA isolates at 3 000 µg/disc. Moreover, the methanol extract of Cotoneaster microphyllus formed a 12.3 mm and 9.1 mm zone of inhibition against the MSSA and MRSA isolates, respectively. |
| first_indexed | 2024-03-12T21:15:04Z |
| format | Article |
| id | doaj.art-90e0841d5e7244e7b1fb8d50ba0abd1c |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-03-12T21:15:04Z |
| publishDate | 2023-07-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-90e0841d5e7244e7b1fb8d50ba0abd1c2023-07-29T20:52:55ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922023-07-0168727128010.17221/80/2022-VETMEDvet-202307-0002Therapeutic potential of natural products and antibiotics against bovine mastitis pathogen of cows and buffaloesW Khan0SA Khan1FA Khan2S Khan3I Ullah4A Shah5I Uddin6S Khan7H Khan8MN Uddin9F Akbar10N Qayum11Centre for Biotechnology and Microbiology, University of Swat, Swat, PakistanCentre for Biotechnology and Microbiology, University of Swat, Swat, PakistanCentre for Biotechnology and Microbiology, University of Swat, Swat, PakistanCentre for Biotechnology and Microbiology, University of Swat, Swat, PakistanCentre for Biotechnology and Microbiology, University of Swat, Swat, PakistanCentre for Biotechnology and Microbiology, University of Swat, Swat, PakistanVeterinary Research and Disease Investigation Center, Balogram, Swat, PakistanVeterinary Research and Disease Investigation Center, Balogram, Swat, PakistanCentre for Biotechnology and Microbiology, University of Swat, Swat, PakistanCentre for Biotechnology and Microbiology, University of Swat, Swat, PakistanCentre for Biotechnology and Microbiology, University of Swat, Swat, PakistanCentre for Biotechnology and Microbiology, University of Swat, Swat, PakistanThe present study aims to evaluate the prevalence and antimicrobial sensitivity of Staphylococcus aureus associated with bovine mastitis to selected antibiotics and plant extracts. In the current study, 140 milk samples were collected from cows and buffaloes. Among the 140 samples, 93 samples were positive for sub-clinical mastitis based on the California Mastitis Test (CMT). Out of the total positive samples, 45 were confirmed for S. aureus on a Mannitol salt agar media. The antimicrobial susceptibility test revealed that 44.82% of the isolates were resistant to cefoxitin (oxacillin) confirming methicillin-resistant S. aureus (MRSA) with a higher percentage (51.61%) in the buffalo than in the cow samples. Furthermore, the PCR assay confirmed the presence of the mecA gene in all the MRSA isolates. Among the seven tested antibiotics, sulfamethoxazole + trimethoprim showed high efficacy (71.1%) against methicillin-susceptible S. aureus isolates (MSSA). Oxytetracycline and sulfamethoxazole + trimethoprim showed 20% efficacy against MRSA followed by enrofloxacin (10%). On the other hand, the tested samples from Pistacia chinensis revealed that the ethyl acetate extract of bark showed a maximum zone of inhibition of 21.3 mm against MSSA and MRSA isolates at 3 000 µg/disc. Moreover, the methanol extract of Cotoneaster microphyllus formed a 12.3 mm and 9.1 mm zone of inhibition against the MSSA and MRSA isolates, respectively.https://vetmed.agriculturejournals.cz/artkey/vet-202307-0002_therapeutic-potential-of-natural-products-and-antibiotics-against-bovine-mastitis-pathogen-of-cows-and-buffaloe.phpantibacterial activitycotoneaster microphyllusmeca genepistacia chinensisstaphylococcus aureus |
| spellingShingle | W Khan SA Khan FA Khan S Khan I Ullah A Shah I Uddin S Khan H Khan MN Uddin F Akbar N Qayum Therapeutic potential of natural products and antibiotics against bovine mastitis pathogen of cows and buffaloes Veterinární Medicína antibacterial activity cotoneaster microphyllus meca gene pistacia chinensis staphylococcus aureus |
| title | Therapeutic potential of natural products and antibiotics against bovine mastitis pathogen of cows and buffaloes |
| title_full | Therapeutic potential of natural products and antibiotics against bovine mastitis pathogen of cows and buffaloes |
| title_fullStr | Therapeutic potential of natural products and antibiotics against bovine mastitis pathogen of cows and buffaloes |
| title_full_unstemmed | Therapeutic potential of natural products and antibiotics against bovine mastitis pathogen of cows and buffaloes |
| title_short | Therapeutic potential of natural products and antibiotics against bovine mastitis pathogen of cows and buffaloes |
| title_sort | therapeutic potential of natural products and antibiotics against bovine mastitis pathogen of cows and buffaloes |
| topic | antibacterial activity cotoneaster microphyllus meca gene pistacia chinensis staphylococcus aureus |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-202307-0002_therapeutic-potential-of-natural-products-and-antibiotics-against-bovine-mastitis-pathogen-of-cows-and-buffaloe.php |
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