Phytochemical Characterization and Antifungal Efficacy of Camphor (<i>Cinnamomum camphora</i> L.) Extract against Phytopathogenic Fungi
<i>Cinnamomum camphora</i> methanolic extract was tested for antifungal activity against three common, isolated, widespread phytopathogens: <i>Alternaria alternata</i>, <i>Fusarium solani</i>, and <i>Fusarium oxysporum</i>, which were molecularly ident...
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MDPI AG
2023-03-01
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| author | Sherien Sobhy Abdulaziz A. Al-Askar Elsayed K. Bakhiet Mohsen M. Elsharkawy Amr A. Arishi Said I. Behiry Ahmed Abdelkhalek |
| author_facet | Sherien Sobhy Abdulaziz A. Al-Askar Elsayed K. Bakhiet Mohsen M. Elsharkawy Amr A. Arishi Said I. Behiry Ahmed Abdelkhalek |
| author_sort | Sherien Sobhy |
| collection | DOAJ |
| description | <i>Cinnamomum camphora</i> methanolic extract was tested for antifungal activity against three common, isolated, widespread phytopathogens: <i>Alternaria alternata</i>, <i>Fusarium solani</i>, and <i>Fusarium oxysporum</i>, which were molecularly identified and assigned accession numbers ON795987, ON795988, and ON795989, respectively. At 4000 µg/mL, the highest concentration of <i>C. camphora</i> methanolic extract inhibits the fungal mycelia weight of <i>F. oxysporum</i>, <i>A. alternata</i>, and <i>F. solani</i> by 60, 49, and 24%, respectively. The presence of several bioactive metabolites in the <i>C. camphora</i> extract could explain its antifungal activity. The presence of numerous phenolic and flavonoid compounds in the extract was revealed by HPLC analysis, including catechin and gallic acid, which had the highest concentrations of 6.21 and 6.98 µg/mL, respectively. Furthermore, osmoprotectants, total amino acids, and glycine betaine were abundant. Furthermore, total antioxidant activities, as measured by PMA and DPPH, were significant. The most abundant compound in the extract, according to GC-MS analysis, was mono(2-ethylhexyl) ester of 1,2-benzene dicarboxylic acid. Based on its in vitro efficacy in inhibiting mycelial growth weight, the tested extract could be recommended as a safe fungicide instead of a chemical treatment. |
| first_indexed | 2024-03-11T05:55:28Z |
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| language | English |
| last_indexed | 2024-03-11T05:55:28Z |
| publishDate | 2023-03-01 |
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| series | Separations |
| spelling | doaj.art-90e30d09838b4b87bccaed97f73dd35e2023-11-17T13:50:17ZengMDPI AGSeparations2297-87392023-03-0110318910.3390/separations10030189Phytochemical Characterization and Antifungal Efficacy of Camphor (<i>Cinnamomum camphora</i> L.) Extract against Phytopathogenic FungiSherien Sobhy0Abdulaziz A. Al-Askar1Elsayed K. Bakhiet2Mohsen M. Elsharkawy3Amr A. Arishi4Said I. Behiry5Ahmed Abdelkhalek6Plant Protection and Biomolecular Diagnosis Department, ALCRI—Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El Arab City, Alexandria 21934, EgyptDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut 71524, EgyptDepartment of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, EgyptSchool of Molecular Sciences, The University of Western Australia, Perth, WA 6009, AustraliaAgricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, EgyptPlant Protection and Biomolecular Diagnosis Department, ALCRI—Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El Arab City, Alexandria 21934, Egypt<i>Cinnamomum camphora</i> methanolic extract was tested for antifungal activity against three common, isolated, widespread phytopathogens: <i>Alternaria alternata</i>, <i>Fusarium solani</i>, and <i>Fusarium oxysporum</i>, which were molecularly identified and assigned accession numbers ON795987, ON795988, and ON795989, respectively. At 4000 µg/mL, the highest concentration of <i>C. camphora</i> methanolic extract inhibits the fungal mycelia weight of <i>F. oxysporum</i>, <i>A. alternata</i>, and <i>F. solani</i> by 60, 49, and 24%, respectively. The presence of several bioactive metabolites in the <i>C. camphora</i> extract could explain its antifungal activity. The presence of numerous phenolic and flavonoid compounds in the extract was revealed by HPLC analysis, including catechin and gallic acid, which had the highest concentrations of 6.21 and 6.98 µg/mL, respectively. Furthermore, osmoprotectants, total amino acids, and glycine betaine were abundant. Furthermore, total antioxidant activities, as measured by PMA and DPPH, were significant. The most abundant compound in the extract, according to GC-MS analysis, was mono(2-ethylhexyl) ester of 1,2-benzene dicarboxylic acid. Based on its in vitro efficacy in inhibiting mycelial growth weight, the tested extract could be recommended as a safe fungicide instead of a chemical treatment.https://www.mdpi.com/2297-8739/10/3/189<i>Cinnamomum camphora</i>HPLCGC-MSphenolic compoundsflavonoid compoundsplant extract |
| spellingShingle | Sherien Sobhy Abdulaziz A. Al-Askar Elsayed K. Bakhiet Mohsen M. Elsharkawy Amr A. Arishi Said I. Behiry Ahmed Abdelkhalek Phytochemical Characterization and Antifungal Efficacy of Camphor (<i>Cinnamomum camphora</i> L.) Extract against Phytopathogenic Fungi Separations <i>Cinnamomum camphora</i> HPLC GC-MS phenolic compounds flavonoid compounds plant extract |
| title | Phytochemical Characterization and Antifungal Efficacy of Camphor (<i>Cinnamomum camphora</i> L.) Extract against Phytopathogenic Fungi |
| title_full | Phytochemical Characterization and Antifungal Efficacy of Camphor (<i>Cinnamomum camphora</i> L.) Extract against Phytopathogenic Fungi |
| title_fullStr | Phytochemical Characterization and Antifungal Efficacy of Camphor (<i>Cinnamomum camphora</i> L.) Extract against Phytopathogenic Fungi |
| title_full_unstemmed | Phytochemical Characterization and Antifungal Efficacy of Camphor (<i>Cinnamomum camphora</i> L.) Extract against Phytopathogenic Fungi |
| title_short | Phytochemical Characterization and Antifungal Efficacy of Camphor (<i>Cinnamomum camphora</i> L.) Extract against Phytopathogenic Fungi |
| title_sort | phytochemical characterization and antifungal efficacy of camphor i cinnamomum camphora i l extract against phytopathogenic fungi |
| topic | <i>Cinnamomum camphora</i> HPLC GC-MS phenolic compounds flavonoid compounds plant extract |
| url | https://www.mdpi.com/2297-8739/10/3/189 |
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