Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content>
ABSTRACT Escherichia coli mazEF is an extensively studied stress-induced toxin-antitoxin (TA) system. The toxin MazF is an endoribonuclease that cleaves RNAs at ACA sites. By that means, under stress, the induced MazF generates a stress-induced translation machinery (STM) composed of MazF-processed...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2019-04-01
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| Series: | mBio |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mBio.00340-19 |
| _version_ | 1818346790593757184 |
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| author | Akanksha Nigam Tamar Ziv Adi Oron-Gottesman Hanna Engelberg-Kulka |
| author_facet | Akanksha Nigam Tamar Ziv Adi Oron-Gottesman Hanna Engelberg-Kulka |
| author_sort | Akanksha Nigam |
| collection | DOAJ |
| description | ABSTRACT Escherichia coli mazEF is an extensively studied stress-induced toxin-antitoxin (TA) system. The toxin MazF is an endoribonuclease that cleaves RNAs at ACA sites. By that means, under stress, the induced MazF generates a stress-induced translation machinery (STM) composed of MazF-processed mRNAs and selective ribosomes that specifically translate the processed mRNAs. Here, we performed a proteomic analysis of all the E. coli stress-induced proteins that are mediated through the chromosomally borne mazF gene. We show that the mRNAs of almost all of them are characterized by the presence of an ACA site up to 100 nucleotides upstream of the AUG initiator. Therefore, under stressful conditions, induced MazF processes mRNAs that are translated by STM. Furthermore, the presence of the ACA sites far upstream (up to 100 nucleotides) of the AUG initiator may still permit translation by the canonical translation machinery. Thus, such dual-translation mechanisms enable the bacterium under stress also to prepare proteins for immediate functions while coming back to normal growth conditions. IMPORTANCE The stress response, the strategy that bacteria have developed in order to cope up with all kinds of adverse conditions, is so far understood at the level of transcription. Our previous findings of a uniquely modified stress-induced translation machinery (STM) generated in E. coli under stress by the endoribonucleolytic activity of the toxin MazF opens a new chapter in understanding microbial physiology under stress at the translational level. Here, we performed a proteomic analysis of all the E. coli stress-induced proteins that are mediated by chromosomally borne MazF through STM. |
| first_indexed | 2024-12-13T17:23:52Z |
| format | Article |
| id | doaj.art-90e5b8332358454f9d3e124271316e3a |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-12-13T17:23:52Z |
| publishDate | 2019-04-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-90e5b8332358454f9d3e124271316e3a2022-12-21T23:37:15ZengAmerican Society for MicrobiologymBio2150-75112019-04-0110210.1128/mBio.00340-19Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content>Akanksha Nigam0Tamar Ziv1Adi Oron-Gottesman2Hanna Engelberg-Kulka3Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University Hadassah Medical School, Jerusalem, IsraelSmoler Proteomics Center, Faculty of Biology, Technion, Haifa, IsraelDepartment of Microbiology and Molecular Genetics, IMRIC, The Hebrew University Hadassah Medical School, Jerusalem, IsraelDepartment of Microbiology and Molecular Genetics, IMRIC, The Hebrew University Hadassah Medical School, Jerusalem, IsraelABSTRACT Escherichia coli mazEF is an extensively studied stress-induced toxin-antitoxin (TA) system. The toxin MazF is an endoribonuclease that cleaves RNAs at ACA sites. By that means, under stress, the induced MazF generates a stress-induced translation machinery (STM) composed of MazF-processed mRNAs and selective ribosomes that specifically translate the processed mRNAs. Here, we performed a proteomic analysis of all the E. coli stress-induced proteins that are mediated through the chromosomally borne mazF gene. We show that the mRNAs of almost all of them are characterized by the presence of an ACA site up to 100 nucleotides upstream of the AUG initiator. Therefore, under stressful conditions, induced MazF processes mRNAs that are translated by STM. Furthermore, the presence of the ACA sites far upstream (up to 100 nucleotides) of the AUG initiator may still permit translation by the canonical translation machinery. Thus, such dual-translation mechanisms enable the bacterium under stress also to prepare proteins for immediate functions while coming back to normal growth conditions. IMPORTANCE The stress response, the strategy that bacteria have developed in order to cope up with all kinds of adverse conditions, is so far understood at the level of transcription. Our previous findings of a uniquely modified stress-induced translation machinery (STM) generated in E. coli under stress by the endoribonucleolytic activity of the toxin MazF opens a new chapter in understanding microbial physiology under stress at the translational level. Here, we performed a proteomic analysis of all the E. coli stress-induced proteins that are mediated by chromosomally borne MazF through STM.https://journals.asm.org/doi/10.1128/mBio.00340-19bacterial stress responsetoxin-antitoxinproteomics |
| spellingShingle | Akanksha Nigam Tamar Ziv Adi Oron-Gottesman Hanna Engelberg-Kulka Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content> mBio bacterial stress response toxin-antitoxin proteomics |
| title | Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content> |
| title_full | Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content> |
| title_fullStr | Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content> |
| title_full_unstemmed | Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content> |
| title_short | Stress-Induced MazF-Mediated Proteins in <named-content content-type="genus-species">Escherichia coli</named-content> |
| title_sort | stress induced mazf mediated proteins in named content content type genus species escherichia coli named content |
| topic | bacterial stress response toxin-antitoxin proteomics |
| url | https://journals.asm.org/doi/10.1128/mBio.00340-19 |
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