Effect of Co-presentation of Adhesive Ligands and Short Hyaluronan on Lymphendothelial Cells
Controlled activation of lymphangiogenesis through functional biomaterials represents a promising approach to support wound healing after surgical procedures, yet remains a challenge. In a synthetic biological approach, we therefore set out to mimic the basal microenvironment of human primary dermal...
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| Format: | Article |
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fbioe.2018.00025/full |
| _version_ | 1818295491566239744 |
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| author | Christiane H. Antoni Christiane H. Antoni Yvonne McDuffie Yvonne McDuffie Jochen Bauer Jonathan P. Sleeman Jonathan P. Sleeman Heike Boehm Heike Boehm |
| author_facet | Christiane H. Antoni Christiane H. Antoni Yvonne McDuffie Yvonne McDuffie Jochen Bauer Jonathan P. Sleeman Jonathan P. Sleeman Heike Boehm Heike Boehm |
| author_sort | Christiane H. Antoni |
| collection | DOAJ |
| description | Controlled activation of lymphangiogenesis through functional biomaterials represents a promising approach to support wound healing after surgical procedures, yet remains a challenge. In a synthetic biological approach, we therefore set out to mimic the basal microenvironment of human primary dermal lymphatic endothelial cells (LECs) during lymphangiogenesis. As the extracellular matrix component hyaluronan (HA) regulates lymphangiogenesis, we designed a bifunctional surface in which adhesive peptide ligands and short HA oligosaccharides (sHA) tethered to nanoparticles are copresented to the basal side of LECs in a controlled, concentration-dependent manner. Exposure of LECs to sHA in solution to mimic luminal stimulation of the cells did not result in modified metabolic activity. However, LECs grown on the bifunctional adhesive surfaces showed a biphasic change in metabolic activity, with increased metabolic activity being observed in response to increasing nanoparticle densities up to a maximum of 540 particles/μm2. Thus, interfaces that concomitantly present adhesive ligands and sHA can stimulate LEC metabolism and might be able to trigger lymphangiogenesis. |
| first_indexed | 2024-12-13T03:48:29Z |
| format | Article |
| id | doaj.art-90e60a7cb34441288079b0da794cc98b |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-13T03:48:29Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-90e60a7cb34441288079b0da794cc98b2022-12-22T00:00:45ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852018-03-01610.3389/fbioe.2018.00025340682Effect of Co-presentation of Adhesive Ligands and Short Hyaluronan on Lymphendothelial CellsChristiane H. Antoni0Christiane H. Antoni1Yvonne McDuffie2Yvonne McDuffie3Jochen Bauer4Jonathan P. Sleeman5Jonathan P. Sleeman6Heike Boehm7Heike Boehm8Department of Cellular Biophysics, Max Planck Institute for Medical Research, Heidelberg, GermanyDepartment of Biophysical Chemistry, University of Heidelberg, Heidelberg, GermanyDepartment of Cellular Biophysics, Max Planck Institute for Medical Research, Heidelberg, GermanyDepartment of Biophysical Chemistry, University of Heidelberg, Heidelberg, GermanyInstitute of Toxicology and Genetics, Karlsruhe Institute for Technology (KIT), Karlsruhe, GermanyInstitute of Toxicology and Genetics, Karlsruhe Institute for Technology (KIT), Karlsruhe, GermanyMedical Faculty Mannheim, University of Heidelberg, Mannheim, GermanyDepartment of Cellular Biophysics, Max Planck Institute for Medical Research, Heidelberg, GermanyDepartment of Biophysical Chemistry, University of Heidelberg, Heidelberg, GermanyControlled activation of lymphangiogenesis through functional biomaterials represents a promising approach to support wound healing after surgical procedures, yet remains a challenge. In a synthetic biological approach, we therefore set out to mimic the basal microenvironment of human primary dermal lymphatic endothelial cells (LECs) during lymphangiogenesis. As the extracellular matrix component hyaluronan (HA) regulates lymphangiogenesis, we designed a bifunctional surface in which adhesive peptide ligands and short HA oligosaccharides (sHA) tethered to nanoparticles are copresented to the basal side of LECs in a controlled, concentration-dependent manner. Exposure of LECs to sHA in solution to mimic luminal stimulation of the cells did not result in modified metabolic activity. However, LECs grown on the bifunctional adhesive surfaces showed a biphasic change in metabolic activity, with increased metabolic activity being observed in response to increasing nanoparticle densities up to a maximum of 540 particles/μm2. Thus, interfaces that concomitantly present adhesive ligands and sHA can stimulate LEC metabolism and might be able to trigger lymphangiogenesis.http://journal.frontiersin.org/article/10.3389/fbioe.2018.00025/fullhyaluronanlymphangiogenesisextracellular matrix mimeticlymphendothelial cellsbioactive interface |
| spellingShingle | Christiane H. Antoni Christiane H. Antoni Yvonne McDuffie Yvonne McDuffie Jochen Bauer Jonathan P. Sleeman Jonathan P. Sleeman Heike Boehm Heike Boehm Effect of Co-presentation of Adhesive Ligands and Short Hyaluronan on Lymphendothelial Cells Frontiers in Bioengineering and Biotechnology hyaluronan lymphangiogenesis extracellular matrix mimetic lymphendothelial cells bioactive interface |
| title | Effect of Co-presentation of Adhesive Ligands and Short Hyaluronan on Lymphendothelial Cells |
| title_full | Effect of Co-presentation of Adhesive Ligands and Short Hyaluronan on Lymphendothelial Cells |
| title_fullStr | Effect of Co-presentation of Adhesive Ligands and Short Hyaluronan on Lymphendothelial Cells |
| title_full_unstemmed | Effect of Co-presentation of Adhesive Ligands and Short Hyaluronan on Lymphendothelial Cells |
| title_short | Effect of Co-presentation of Adhesive Ligands and Short Hyaluronan on Lymphendothelial Cells |
| title_sort | effect of co presentation of adhesive ligands and short hyaluronan on lymphendothelial cells |
| topic | hyaluronan lymphangiogenesis extracellular matrix mimetic lymphendothelial cells bioactive interface |
| url | http://journal.frontiersin.org/article/10.3389/fbioe.2018.00025/full |
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