The presence of plasmids in Aeromonas hydrophila and its relationship with antimicrobial and heavy metal-resistance profiles
ABSTRACT: Aeromonas hydrophila is a common fish pathogen that causes extensive damage to aquaculture. To develop and implement a more adequate strategy to farm fish, it is crucial to understand the bacterial-resistance levels and their transference dynamics. The objective of this study was to analy...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
2018-09-01
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| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900451&lng=en&tlng=en |
| _version_ | 1818294110680776704 |
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| author | Mariana Ramos Freitas Naiana Braga Freire Luciana Jatobá e Silva Peixoto Samira Teixeira Leal de Oliveira Renilde Cordeiro de Souza João José de Simoni Gouveia Mateus Matiuzzi da Costa Gisele Veneroni Gouveia |
| author_facet | Mariana Ramos Freitas Naiana Braga Freire Luciana Jatobá e Silva Peixoto Samira Teixeira Leal de Oliveira Renilde Cordeiro de Souza João José de Simoni Gouveia Mateus Matiuzzi da Costa Gisele Veneroni Gouveia |
| author_sort | Mariana Ramos Freitas |
| collection | DOAJ |
| description | ABSTRACT: Aeromonas hydrophila is a common fish pathogen that causes extensive damage to aquaculture. To develop and implement a more adequate strategy to farm fish, it is crucial to understand the bacterial-resistance levels and their transference dynamics. The objective of this study was to analyze the resistance profile of isolated Aeromonas hydrophila to antimicrobial agents and heavy metals and draw a correlation of the observed profiles with the presence of plasmids. Resistance of the isolated bacteria to antimicrobial agents (oxacilin, gentamicin, tetracycline, and nalidixic acid) and heavy metals (cadmium, lead, copper, and manganese) was verified using the minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) standards. The Multiple Antibiotic Resistance Index (MAR Index) was calculated. Plasmids were extracted by using a common methodology described elsewhere. Mann-Whitney Test, implemented in the R environment, was used to determine the correlation between resistance and plasmids presence. A high resistance to almost all antimicrobial agents and heavy metals was observed, except to gentamicin and cadmium. The MAR index results showed resistance to all antimicrobial profiles. Of the isolated bacteria, 14 showed the presence of plasmids. However, no correlation was noted between the resistance profile and the plasmid presence for these isolates, indicating that the genes responsible for resistance to microbial agents and heavy metals are present in the cromossomic DNA, which in turn suggested the possibility of gene transfer between the isolated bacteria. The resistance to heavy metals can be linked to heavy utilization of fertilizers along the Sao Francisco River. |
| first_indexed | 2024-12-13T03:26:33Z |
| format | Article |
| id | doaj.art-90ea4068b5b64d27b38a8e280959eb83 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-12-13T03:26:33Z |
| publishDate | 2018-09-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-90ea4068b5b64d27b38a8e280959eb832022-12-22T00:01:15ZengUniversidade Federal de Santa MariaCiência Rural1678-45962018-09-0148910.1590/0103-8478cr20170813S0103-84782018000900451The presence of plasmids in Aeromonas hydrophila and its relationship with antimicrobial and heavy metal-resistance profilesMariana Ramos FreitasNaiana Braga FreireLuciana Jatobá e Silva PeixotoSamira Teixeira Leal de OliveiraRenilde Cordeiro de SouzaJoão José de Simoni GouveiaMateus Matiuzzi da CostaGisele Veneroni GouveiaABSTRACT: Aeromonas hydrophila is a common fish pathogen that causes extensive damage to aquaculture. To develop and implement a more adequate strategy to farm fish, it is crucial to understand the bacterial-resistance levels and their transference dynamics. The objective of this study was to analyze the resistance profile of isolated Aeromonas hydrophila to antimicrobial agents and heavy metals and draw a correlation of the observed profiles with the presence of plasmids. Resistance of the isolated bacteria to antimicrobial agents (oxacilin, gentamicin, tetracycline, and nalidixic acid) and heavy metals (cadmium, lead, copper, and manganese) was verified using the minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) standards. The Multiple Antibiotic Resistance Index (MAR Index) was calculated. Plasmids were extracted by using a common methodology described elsewhere. Mann-Whitney Test, implemented in the R environment, was used to determine the correlation between resistance and plasmids presence. A high resistance to almost all antimicrobial agents and heavy metals was observed, except to gentamicin and cadmium. The MAR index results showed resistance to all antimicrobial profiles. Of the isolated bacteria, 14 showed the presence of plasmids. However, no correlation was noted between the resistance profile and the plasmid presence for these isolates, indicating that the genes responsible for resistance to microbial agents and heavy metals are present in the cromossomic DNA, which in turn suggested the possibility of gene transfer between the isolated bacteria. The resistance to heavy metals can be linked to heavy utilization of fertilizers along the Sao Francisco River.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900451&lng=en&tlng=enaquiculturamultirresistênciamegaplasmídeosDNA cromossomalintegrons |
| spellingShingle | Mariana Ramos Freitas Naiana Braga Freire Luciana Jatobá e Silva Peixoto Samira Teixeira Leal de Oliveira Renilde Cordeiro de Souza João José de Simoni Gouveia Mateus Matiuzzi da Costa Gisele Veneroni Gouveia The presence of plasmids in Aeromonas hydrophila and its relationship with antimicrobial and heavy metal-resistance profiles Ciência Rural aquicultura multirresistência megaplasmídeos DNA cromossomal integrons |
| title | The presence of plasmids in Aeromonas hydrophila and its relationship with antimicrobial and heavy metal-resistance profiles |
| title_full | The presence of plasmids in Aeromonas hydrophila and its relationship with antimicrobial and heavy metal-resistance profiles |
| title_fullStr | The presence of plasmids in Aeromonas hydrophila and its relationship with antimicrobial and heavy metal-resistance profiles |
| title_full_unstemmed | The presence of plasmids in Aeromonas hydrophila and its relationship with antimicrobial and heavy metal-resistance profiles |
| title_short | The presence of plasmids in Aeromonas hydrophila and its relationship with antimicrobial and heavy metal-resistance profiles |
| title_sort | presence of plasmids in aeromonas hydrophila and its relationship with antimicrobial and heavy metal resistance profiles |
| topic | aquicultura multirresistência megaplasmídeos DNA cromossomal integrons |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900451&lng=en&tlng=en |
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