Antibacterial Activity and Multi-Targeting Mechanism of Dehydrocorydaline From Corydalis turtschaninovii Bess. Against Listeria monocytogenes
Listeria monocytogenes is a foodborne pathogen, with relatively low incidence but high case-fatality. Phytochemicals have been recognized as a promising antimicrobial agent as an alternative to synthetic chemicals due to their safety and high efficacy with multi-target sites. This study identified a...
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| Format: | Article |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.799094/full |
| _version_ | 1798035918438793216 |
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| author | Gowoon Kim Yijuan Xu Jiarong Zhang Zhongquan Sui Harold Corke Harold Corke |
| author_facet | Gowoon Kim Yijuan Xu Jiarong Zhang Zhongquan Sui Harold Corke Harold Corke |
| author_sort | Gowoon Kim |
| collection | DOAJ |
| description | Listeria monocytogenes is a foodborne pathogen, with relatively low incidence but high case-fatality. Phytochemicals have been recognized as a promising antimicrobial agent as an alternative to synthetic chemicals due to their safety and high efficacy with multi-target sites. This study identified and characterized a novel antibacterial agent, dehydrocorydaline, in the Corydalis turschaninovii rhizome using HPLC-LTQ-Orbitrap-HRMS, and its antibacterial effect with lowest MIC (1 mg/mL) and MBC (2 mg/mL) values. In addition, an in vitro growth kinetic assay, cytoplasmic nucleic acid and protein leakage assay, and observation of morphological changes in bacterial cells supported the strong antibacterial activity. Dehydrocorydaline also displayed effective inhibitory effects on biofilm formation and bacterial motility. In order to investigate the potential antibacterial mechanism of action of dehydrocorydaline against L. monocytogenes, label-free quantitative proteomics was used, demonstrating that dehydrocorydaline has multiple targets for combating L. monocytogenes including dysregulation of carbohydrate metabolism, suppression of cell wall synthesis, and inhibition of bacterial motility. Overall, this study demonstrated that dehydrocorydaline has potential as a natural and effective antibacterial agent with multi-target sites in pathogenic bacteria, and provides the basis for development of a new class of antibacterial agent. |
| first_indexed | 2024-04-11T21:05:06Z |
| format | Article |
| id | doaj.art-90ec4d3bf7934169ae5400554e0a7d7e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T21:05:06Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-90ec4d3bf7934169ae5400554e0a7d7e2022-12-22T04:03:23ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-01-011210.3389/fmicb.2021.799094799094Antibacterial Activity and Multi-Targeting Mechanism of Dehydrocorydaline From Corydalis turtschaninovii Bess. Against Listeria monocytogenesGowoon Kim0Yijuan Xu1Jiarong Zhang2Zhongquan Sui3Harold Corke4Harold Corke5Department of Food Science and Technology, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Food Science and Technology, Shanghai Jiao Tong University, Shanghai, ChinaBiotechnology and Food Engineering Program, Guangdong Technion – Israel Institute of Technology, Shantou, ChinaDepartment of Food Science and Technology, Shanghai Jiao Tong University, Shanghai, ChinaBiotechnology and Food Engineering Program, Guangdong Technion – Israel Institute of Technology, Shantou, ChinaFaculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, IsraelListeria monocytogenes is a foodborne pathogen, with relatively low incidence but high case-fatality. Phytochemicals have been recognized as a promising antimicrobial agent as an alternative to synthetic chemicals due to their safety and high efficacy with multi-target sites. This study identified and characterized a novel antibacterial agent, dehydrocorydaline, in the Corydalis turschaninovii rhizome using HPLC-LTQ-Orbitrap-HRMS, and its antibacterial effect with lowest MIC (1 mg/mL) and MBC (2 mg/mL) values. In addition, an in vitro growth kinetic assay, cytoplasmic nucleic acid and protein leakage assay, and observation of morphological changes in bacterial cells supported the strong antibacterial activity. Dehydrocorydaline also displayed effective inhibitory effects on biofilm formation and bacterial motility. In order to investigate the potential antibacterial mechanism of action of dehydrocorydaline against L. monocytogenes, label-free quantitative proteomics was used, demonstrating that dehydrocorydaline has multiple targets for combating L. monocytogenes including dysregulation of carbohydrate metabolism, suppression of cell wall synthesis, and inhibition of bacterial motility. Overall, this study demonstrated that dehydrocorydaline has potential as a natural and effective antibacterial agent with multi-target sites in pathogenic bacteria, and provides the basis for development of a new class of antibacterial agent.https://www.frontiersin.org/articles/10.3389/fmicb.2021.799094/fullfoodborne pathogenphytochemicalsalkaloidsantibacterial mechanismproteomic analysis |
| spellingShingle | Gowoon Kim Yijuan Xu Jiarong Zhang Zhongquan Sui Harold Corke Harold Corke Antibacterial Activity and Multi-Targeting Mechanism of Dehydrocorydaline From Corydalis turtschaninovii Bess. Against Listeria monocytogenes Frontiers in Microbiology foodborne pathogen phytochemicals alkaloids antibacterial mechanism proteomic analysis |
| title | Antibacterial Activity and Multi-Targeting Mechanism of Dehydrocorydaline From Corydalis turtschaninovii Bess. Against Listeria monocytogenes |
| title_full | Antibacterial Activity and Multi-Targeting Mechanism of Dehydrocorydaline From Corydalis turtschaninovii Bess. Against Listeria monocytogenes |
| title_fullStr | Antibacterial Activity and Multi-Targeting Mechanism of Dehydrocorydaline From Corydalis turtschaninovii Bess. Against Listeria monocytogenes |
| title_full_unstemmed | Antibacterial Activity and Multi-Targeting Mechanism of Dehydrocorydaline From Corydalis turtschaninovii Bess. Against Listeria monocytogenes |
| title_short | Antibacterial Activity and Multi-Targeting Mechanism of Dehydrocorydaline From Corydalis turtschaninovii Bess. Against Listeria monocytogenes |
| title_sort | antibacterial activity and multi targeting mechanism of dehydrocorydaline from corydalis turtschaninovii bess against listeria monocytogenes |
| topic | foodborne pathogen phytochemicals alkaloids antibacterial mechanism proteomic analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.799094/full |
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