The study of different markers among Chuanxiong Chatiao dosage forms based on bioactive components and antiplatelet aggregation biopotency integrated with chemometrics
Chuanxiong Chatiao powder is a classic prescription in traditional Chinese medicine. A variety of dosage forms have been developed based on powders, such as dropping pills, granules, tablets, pills, and concentrated pills. This classic prescription consists of various active constituents, but due to...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
|
| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223008833 |
| _version_ | 1797390558732222464 |
|---|---|
| author | Jia-jia Xiao Yu-ping Weng Long-biao Luo Jing-qi Wang Huan-zhao Lin Guang-mou Zhang Lin Yue Yi-xin Yao |
| author_facet | Jia-jia Xiao Yu-ping Weng Long-biao Luo Jing-qi Wang Huan-zhao Lin Guang-mou Zhang Lin Yue Yi-xin Yao |
| author_sort | Jia-jia Xiao |
| collection | DOAJ |
| description | Chuanxiong Chatiao powder is a classic prescription in traditional Chinese medicine. A variety of dosage forms have been developed based on powders, such as dropping pills, granules, tablets, pills, and concentrated pills. This classic prescription consists of various active constituents, but due to the selection of a single and unreasonable quality standard indicator, may cause a difference in active constituent and bioactivity among different dosage forms. However, related reports on the comparisons of the different dosage forms are few. Therefore, high-performance liquid chromatography with triple quadrupole mass spectrometry was developed for the determination of 26 bioactive components of the six Chuanxiong Chatiao dosage forms, and a parallel line assay based on a quantitative response method for biological assays was established to measure the antiplatelet aggregation biopotency (AAB) in vitro. The results indicated that the AAB of the powder was greatest, followed by that of the pills, concentrated pills, dropping pills, tablets, granules. The results were analyzed by hierarchical clustering analysis, showing that were some differences among six dosage forms. 12 different markers, including Z- ligustilide, senkyunolide A, butylidenephthalide, asarinin, rosmarinic acid, pulegone, senkyunolide H, notopterol, coniferyl ferulate, hesperidin, vanillic acid, luteolin-7-O-glucoside, were further screened using partial least squares. All of different markers showed a positive association with AAB. There were significant differences in both the sum and composition of different markers among the different dosage forms. This can provide a foundation for the clinical study of the six Chuanxiong Chatiao dosage forms. |
| first_indexed | 2024-03-08T23:12:38Z |
| format | Article |
| id | doaj.art-90f2578460e848dba13682f5f6bcb933 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-08T23:12:38Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-90f2578460e848dba13682f5f6bcb9332023-12-15T07:23:29ZengElsevierArabian Journal of Chemistry1878-53522024-01-01171105421The study of different markers among Chuanxiong Chatiao dosage forms based on bioactive components and antiplatelet aggregation biopotency integrated with chemometricsJia-jia Xiao0Yu-ping Weng1Long-biao Luo2Jing-qi Wang3Huan-zhao Lin4Guang-mou Zhang5Lin Yue6Yi-xin Yao7Department of TCM Pharmacy, Chengdu Integrated TCM & Western Medicine Hospital, Chengdu 610041, ChinaKangmei Pharmaceutical Co., Ltd, Shenzhen 518000, ChinaState Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical Sciences, University of Macau, Macau 519000, ChinaState Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical Sciences, University of Macau, Macau 519000, ChinaKangmei Pharmaceutical Co., Ltd, Shenzhen 518000, ChinaCollege of Life Science and Technology, Xinxiang Medical University, Xinxiang 453000, ChinaSchool of Nursing, Hunan University of Medicine, Huaihua 418000, China; Corresponding authors.Kangmei Pharmaceutical Co., Ltd, Shenzhen 518000, China; Corresponding authors.Chuanxiong Chatiao powder is a classic prescription in traditional Chinese medicine. A variety of dosage forms have been developed based on powders, such as dropping pills, granules, tablets, pills, and concentrated pills. This classic prescription consists of various active constituents, but due to the selection of a single and unreasonable quality standard indicator, may cause a difference in active constituent and bioactivity among different dosage forms. However, related reports on the comparisons of the different dosage forms are few. Therefore, high-performance liquid chromatography with triple quadrupole mass spectrometry was developed for the determination of 26 bioactive components of the six Chuanxiong Chatiao dosage forms, and a parallel line assay based on a quantitative response method for biological assays was established to measure the antiplatelet aggregation biopotency (AAB) in vitro. The results indicated that the AAB of the powder was greatest, followed by that of the pills, concentrated pills, dropping pills, tablets, granules. The results were analyzed by hierarchical clustering analysis, showing that were some differences among six dosage forms. 12 different markers, including Z- ligustilide, senkyunolide A, butylidenephthalide, asarinin, rosmarinic acid, pulegone, senkyunolide H, notopterol, coniferyl ferulate, hesperidin, vanillic acid, luteolin-7-O-glucoside, were further screened using partial least squares. All of different markers showed a positive association with AAB. There were significant differences in both the sum and composition of different markers among the different dosage forms. This can provide a foundation for the clinical study of the six Chuanxiong Chatiao dosage forms.http://www.sciencedirect.com/science/article/pii/S1878535223008833Chuanxiong Chatiao dosage formsBioactive componentsQuantificationAntiplatelet aggregation biopotencyBioassayDifferent markers |
| spellingShingle | Jia-jia Xiao Yu-ping Weng Long-biao Luo Jing-qi Wang Huan-zhao Lin Guang-mou Zhang Lin Yue Yi-xin Yao The study of different markers among Chuanxiong Chatiao dosage forms based on bioactive components and antiplatelet aggregation biopotency integrated with chemometrics Arabian Journal of Chemistry Chuanxiong Chatiao dosage forms Bioactive components Quantification Antiplatelet aggregation biopotency Bioassay Different markers |
| title | The study of different markers among Chuanxiong Chatiao dosage forms based on bioactive components and antiplatelet aggregation biopotency integrated with chemometrics |
| title_full | The study of different markers among Chuanxiong Chatiao dosage forms based on bioactive components and antiplatelet aggregation biopotency integrated with chemometrics |
| title_fullStr | The study of different markers among Chuanxiong Chatiao dosage forms based on bioactive components and antiplatelet aggregation biopotency integrated with chemometrics |
| title_full_unstemmed | The study of different markers among Chuanxiong Chatiao dosage forms based on bioactive components and antiplatelet aggregation biopotency integrated with chemometrics |
| title_short | The study of different markers among Chuanxiong Chatiao dosage forms based on bioactive components and antiplatelet aggregation biopotency integrated with chemometrics |
| title_sort | study of different markers among chuanxiong chatiao dosage forms based on bioactive components and antiplatelet aggregation biopotency integrated with chemometrics |
| topic | Chuanxiong Chatiao dosage forms Bioactive components Quantification Antiplatelet aggregation biopotency Bioassay Different markers |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223008833 |
| work_keys_str_mv | AT jiajiaxiao thestudyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT yupingweng thestudyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT longbiaoluo thestudyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT jingqiwang thestudyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT huanzhaolin thestudyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT guangmouzhang thestudyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT linyue thestudyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT yixinyao thestudyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT jiajiaxiao studyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT yupingweng studyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT longbiaoluo studyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT jingqiwang studyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT huanzhaolin studyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT guangmouzhang studyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT linyue studyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics AT yixinyao studyofdifferentmarkersamongchuanxiongchatiaodosageformsbasedonbioactivecomponentsandantiplateletaggregationbiopotencyintegratedwithchemometrics |