Pcdhβ deficiency affects hippocampal CA1 ensemble activity and contextual fear discrimination
Abstract Clustered protocadherins (Pcdhs), a large group of adhesion molecules, are important for axonal projections and dendritic spread, but little is known about how they influence neuronal activity. The Pcdhβ cluster is strongly expressed in the hippocampus, and in vivo Ca2+ imaging in Pcdhβ-def...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-01-01
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| Series: | Molecular Brain |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13041-020-0547-z |
| _version_ | 1818331285409497088 |
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| author | Hirotaka Asai Noriaki Ohkawa Yoshito Saitoh Khaled Ghandour Emi Murayama Hirofumi Nishizono Mina Matsuo Teruyoshi Hirayama Ryosuke Kaneko Shin-ichi Muramatsu Takeshi Yagi Kaoru Inokuchi |
| author_facet | Hirotaka Asai Noriaki Ohkawa Yoshito Saitoh Khaled Ghandour Emi Murayama Hirofumi Nishizono Mina Matsuo Teruyoshi Hirayama Ryosuke Kaneko Shin-ichi Muramatsu Takeshi Yagi Kaoru Inokuchi |
| author_sort | Hirotaka Asai |
| collection | DOAJ |
| description | Abstract Clustered protocadherins (Pcdhs), a large group of adhesion molecules, are important for axonal projections and dendritic spread, but little is known about how they influence neuronal activity. The Pcdhβ cluster is strongly expressed in the hippocampus, and in vivo Ca2+ imaging in Pcdhβ-deficient mice revealed altered activity of neuronal ensembles but not of individual cells in this region in freely moving animals. Specifically, Pcdhβ deficiency increased the number of large-size neuronal ensembles and the proportion of cells shared between ensembles. Furthermore, Pcdhβ-deficient mice exhibited reduced repetitive neuronal population activity during exploration of a novel context and were less able to discriminate contexts in a contextual fear conditioning paradigm. These results suggest that one function of Pcdhβs is to modulate neural ensemble activity in the hippocampus to promote context discrimination. |
| first_indexed | 2024-12-13T13:17:25Z |
| format | Article |
| id | doaj.art-90fae18a48a842c8babeeeab2875ff12 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-12-13T13:17:25Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-90fae18a48a842c8babeeeab2875ff122022-12-21T23:44:30ZengBMCMolecular Brain1756-66062020-01-0113111010.1186/s13041-020-0547-zPcdhβ deficiency affects hippocampal CA1 ensemble activity and contextual fear discriminationHirotaka Asai0Noriaki Ohkawa1Yoshito Saitoh2Khaled Ghandour3Emi Murayama4Hirofumi Nishizono5Mina Matsuo6Teruyoshi Hirayama7Ryosuke Kaneko8Shin-ichi Muramatsu9Takeshi Yagi10Kaoru Inokuchi11Department of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of ToyamaDepartment of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of ToyamaDepartment of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of ToyamaDepartment of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of ToyamaDepartment of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of ToyamaDivision of Animal Experimental Laboratory, Life Science Research Center, University of ToyamaDivision of Animal Experimental Laboratory, Life Science Research Center, University of ToyamaDepartment of Anatomy and Developmental Neurobiology, Tokushima UniversityBioresource Center, Gunma University Graduate School of MedicineDivision of Neurology, Department of Medicine, Jichi Medical UniversityKOKORO-Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka UniversityDepartment of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, University of ToyamaAbstract Clustered protocadherins (Pcdhs), a large group of adhesion molecules, are important for axonal projections and dendritic spread, but little is known about how they influence neuronal activity. The Pcdhβ cluster is strongly expressed in the hippocampus, and in vivo Ca2+ imaging in Pcdhβ-deficient mice revealed altered activity of neuronal ensembles but not of individual cells in this region in freely moving animals. Specifically, Pcdhβ deficiency increased the number of large-size neuronal ensembles and the proportion of cells shared between ensembles. Furthermore, Pcdhβ-deficient mice exhibited reduced repetitive neuronal population activity during exploration of a novel context and were less able to discriminate contexts in a contextual fear conditioning paradigm. These results suggest that one function of Pcdhβs is to modulate neural ensemble activity in the hippocampus to promote context discrimination.https://doi.org/10.1186/s13041-020-0547-zHippocampusCA1Clustered protocadherinPcdhβCa2+ imagingEnsemble |
| spellingShingle | Hirotaka Asai Noriaki Ohkawa Yoshito Saitoh Khaled Ghandour Emi Murayama Hirofumi Nishizono Mina Matsuo Teruyoshi Hirayama Ryosuke Kaneko Shin-ichi Muramatsu Takeshi Yagi Kaoru Inokuchi Pcdhβ deficiency affects hippocampal CA1 ensemble activity and contextual fear discrimination Molecular Brain Hippocampus CA1 Clustered protocadherin Pcdhβ Ca2+ imaging Ensemble |
| title | Pcdhβ deficiency affects hippocampal CA1 ensemble activity and contextual fear discrimination |
| title_full | Pcdhβ deficiency affects hippocampal CA1 ensemble activity and contextual fear discrimination |
| title_fullStr | Pcdhβ deficiency affects hippocampal CA1 ensemble activity and contextual fear discrimination |
| title_full_unstemmed | Pcdhβ deficiency affects hippocampal CA1 ensemble activity and contextual fear discrimination |
| title_short | Pcdhβ deficiency affects hippocampal CA1 ensemble activity and contextual fear discrimination |
| title_sort | pcdhβ deficiency affects hippocampal ca1 ensemble activity and contextual fear discrimination |
| topic | Hippocampus CA1 Clustered protocadherin Pcdhβ Ca2+ imaging Ensemble |
| url | https://doi.org/10.1186/s13041-020-0547-z |
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