Inner Shell of the Chestnut (<i>Castanea crenatta</i>) Suppresses Inflammatory Responses in Ovalbumin-Induced Allergic Asthma Mouse Model
The inner shell of the chestnut (Castanea crenata) contains various polyphenols, which exert beneficial biological effects. Hence, we assessed the anti-inflammatory efficacy of a chestnut inner shell extract (CIE) in ovalbumin (OVA)-induced allergic asthma. We intraperitoneally injected 20 μg of OVA...
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MDPI AG
2022-05-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/10/2067 |
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| author | Chang-Yeop Kim Jeong-Won Kim Jin-Hwa Kim Ji-Soo Jeong Je-Oh Lim Je-Won Ko Tae-Won Kim |
| author_facet | Chang-Yeop Kim Jeong-Won Kim Jin-Hwa Kim Ji-Soo Jeong Je-Oh Lim Je-Won Ko Tae-Won Kim |
| author_sort | Chang-Yeop Kim |
| collection | DOAJ |
| description | The inner shell of the chestnut (Castanea crenata) contains various polyphenols, which exert beneficial biological effects. Hence, we assessed the anti-inflammatory efficacy of a chestnut inner shell extract (CIE) in ovalbumin (OVA)-induced allergic asthma. We intraperitoneally injected 20 μg of OVA with 2 mg of aluminum hydroxide on days 0 and 14. On test days 21, 22, and 23, the mice were treated with aerosolized 1% (<i>w/v</i>) OVA in saline. CIE was administered orally at 100 and 300 mg/kg on days 18–23. CIE significantly reduced inflammatory cytokines and cells and immunoglobulin-E increased by OVA. Anti-inflammatory efficacy was revealed by reduction of inflammatory cell migration and mucus secretion in lung tissue. Further, CIE suppressed the OVA-induced nuclear factor kappa B (NF-κB) phosphorylation. Accordingly, the expression of cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS), and matrix metalloproteinase-9 (MMP-9) were decreased sequentially in lung tissues. CIE alleviated OVA-induced airway inflammation by restraining phosphorylation of NF-κB and the sequentially reduced expression of iNOS, COX-2, leading to reduced MMP-9 expression. These results indicate that CIE has potential as a candidate for alleviating asthma. |
| first_indexed | 2024-03-10T03:14:32Z |
| format | Article |
| id | doaj.art-90fc3aaddd464cb09ac7cf9af3936a06 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T03:14:32Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-90fc3aaddd464cb09ac7cf9af3936a062023-11-23T12:29:31ZengMDPI AGNutrients2072-66432022-05-011410206710.3390/nu14102067Inner Shell of the Chestnut (<i>Castanea crenatta</i>) Suppresses Inflammatory Responses in Ovalbumin-Induced Allergic Asthma Mouse ModelChang-Yeop Kim0Jeong-Won Kim1Jin-Hwa Kim2Ji-Soo Jeong3Je-Oh Lim4Je-Won Ko5Tae-Won Kim6BK21 FOUR Program, College of Veterinary Medicine, Chungnam National University, 99 Daehak-ro, Daejeon 34131, KoreaBK21 FOUR Program, College of Veterinary Medicine, Chungnam National University, 99 Daehak-ro, Daejeon 34131, KoreaBK21 FOUR Program, College of Veterinary Medicine, Chungnam National University, 99 Daehak-ro, Daejeon 34131, KoreaBK21 FOUR Program, College of Veterinary Medicine, Chungnam National University, 99 Daehak-ro, Daejeon 34131, KoreaBK21 FOUR Program, College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, KoreaBK21 FOUR Program, College of Veterinary Medicine, Chungnam National University, 99 Daehak-ro, Daejeon 34131, KoreaBK21 FOUR Program, College of Veterinary Medicine, Chungnam National University, 99 Daehak-ro, Daejeon 34131, KoreaThe inner shell of the chestnut (Castanea crenata) contains various polyphenols, which exert beneficial biological effects. Hence, we assessed the anti-inflammatory efficacy of a chestnut inner shell extract (CIE) in ovalbumin (OVA)-induced allergic asthma. We intraperitoneally injected 20 μg of OVA with 2 mg of aluminum hydroxide on days 0 and 14. On test days 21, 22, and 23, the mice were treated with aerosolized 1% (<i>w/v</i>) OVA in saline. CIE was administered orally at 100 and 300 mg/kg on days 18–23. CIE significantly reduced inflammatory cytokines and cells and immunoglobulin-E increased by OVA. Anti-inflammatory efficacy was revealed by reduction of inflammatory cell migration and mucus secretion in lung tissue. Further, CIE suppressed the OVA-induced nuclear factor kappa B (NF-κB) phosphorylation. Accordingly, the expression of cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS), and matrix metalloproteinase-9 (MMP-9) were decreased sequentially in lung tissues. CIE alleviated OVA-induced airway inflammation by restraining phosphorylation of NF-κB and the sequentially reduced expression of iNOS, COX-2, leading to reduced MMP-9 expression. These results indicate that CIE has potential as a candidate for alleviating asthma.https://www.mdpi.com/2072-6643/14/10/2067asthmachestnut inner shell extractairway inflammationinducible nitric oxide synthasecyclooxygenase-2matrix metalloproteinase-9 |
| spellingShingle | Chang-Yeop Kim Jeong-Won Kim Jin-Hwa Kim Ji-Soo Jeong Je-Oh Lim Je-Won Ko Tae-Won Kim Inner Shell of the Chestnut (<i>Castanea crenatta</i>) Suppresses Inflammatory Responses in Ovalbumin-Induced Allergic Asthma Mouse Model Nutrients asthma chestnut inner shell extract airway inflammation inducible nitric oxide synthase cyclooxygenase-2 matrix metalloproteinase-9 |
| title | Inner Shell of the Chestnut (<i>Castanea crenatta</i>) Suppresses Inflammatory Responses in Ovalbumin-Induced Allergic Asthma Mouse Model |
| title_full | Inner Shell of the Chestnut (<i>Castanea crenatta</i>) Suppresses Inflammatory Responses in Ovalbumin-Induced Allergic Asthma Mouse Model |
| title_fullStr | Inner Shell of the Chestnut (<i>Castanea crenatta</i>) Suppresses Inflammatory Responses in Ovalbumin-Induced Allergic Asthma Mouse Model |
| title_full_unstemmed | Inner Shell of the Chestnut (<i>Castanea crenatta</i>) Suppresses Inflammatory Responses in Ovalbumin-Induced Allergic Asthma Mouse Model |
| title_short | Inner Shell of the Chestnut (<i>Castanea crenatta</i>) Suppresses Inflammatory Responses in Ovalbumin-Induced Allergic Asthma Mouse Model |
| title_sort | inner shell of the chestnut i castanea crenatta i suppresses inflammatory responses in ovalbumin induced allergic asthma mouse model |
| topic | asthma chestnut inner shell extract airway inflammation inducible nitric oxide synthase cyclooxygenase-2 matrix metalloproteinase-9 |
| url | https://www.mdpi.com/2072-6643/14/10/2067 |
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