SS18-SSX Rearrangement in Synovial Sarcoma Using Fluorescent in Situ Hybridization: A Report from an Iranian Population and Literature Review
<strong>Background:</strong> Synovial sarcoma is an aggressive soft tissue sarcoma. It has a wide spectrum of histopathologic patterns and uncertain immunohistochemistry, rendering it a diagnostic challenge. The objective of this study was to investigate the diagnostic utility and impact...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2020-10-01
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| Series: | Middle East Journal of Cancer |
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| Online Access: | https://mejc.sums.ac.ir/article_46787_039552932907cbbeea16d22f5c3c36d4.pdf |
| _version_ | 1818187493052252160 |
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| author | Akbar Safaei Elham Jansar Ahmad Monabati Maral Mokhtari Mehdi Montazer |
| author_facet | Akbar Safaei Elham Jansar Ahmad Monabati Maral Mokhtari Mehdi Montazer |
| author_sort | Akbar Safaei |
| collection | DOAJ |
| description | <strong>Background:</strong> Synovial sarcoma is an aggressive soft tissue sarcoma. It has a wide spectrum of histopathologic patterns and uncertain immunohistochemistry, rendering it a diagnostic challenge. The objective of this study was to investigate the diagnostic utility and impact of SS18-SSX rearrangement evaluation in an Iranian population previously diagnosed with synovial sarcoma without such molecular tests.<br /> <strong> Method:</strong> We conducted this cross-sectional study on 44 formalin-fixed paraffin-embedded tissue blocks obtained from 23 synovial sarcoma patients (males, 69%; mean age, 36.4 years) and 11 cases with other neoplasms as negative controls. We assessed these specimens for SSX-SS18 gene rearrangement by break-apart fluorescent in situ hybridization (FISH) probes.<br /> <strong>Results:</strong> FISH study showed SS18-SSX fusion gene in 17 (73.91%) cases while six (26.09%) cases and negative controls did not show SS18-SSX fusion. Histopathologic type of tumor was significantly related to the presence of rearrangement (P=0.002) (rearranged: 11 biphasic and six monophasic tumors; non-rearranged: three monophasic and three poorly-differentiated tumors).<br /> <strong>Conclusion:</strong> This study supports the idea that molecular studies contribute to the confirmation of synovial sarcoma diagnosis, particularly in monophasic and poorly-differentiated subtypes, which show vague immunohistochemical results. |
| first_indexed | 2024-12-11T23:11:54Z |
| format | Article |
| id | doaj.art-90fd97cf5b7c4ebe921852a211c8ef2e |
| institution | Directory Open Access Journal |
| issn | 2008-6709 2008-6687 |
| language | English |
| last_indexed | 2024-12-11T23:11:54Z |
| publishDate | 2020-10-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Middle East Journal of Cancer |
| spelling | doaj.art-90fd97cf5b7c4ebe921852a211c8ef2e2022-12-22T00:46:40ZengShiraz University of Medical SciencesMiddle East Journal of Cancer2008-67092008-66872020-10-0111447648210.30476/mejc.2020.82264.107346787SS18-SSX Rearrangement in Synovial Sarcoma Using Fluorescent in Situ Hybridization: A Report from an Iranian Population and Literature ReviewAkbar Safaei0Elham Jansar1Ahmad Monabati2Maral Mokhtari3Mehdi Montazer4Department of Pathology, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Pathology, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Pathology, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Pathology, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran<strong>Background:</strong> Synovial sarcoma is an aggressive soft tissue sarcoma. It has a wide spectrum of histopathologic patterns and uncertain immunohistochemistry, rendering it a diagnostic challenge. The objective of this study was to investigate the diagnostic utility and impact of SS18-SSX rearrangement evaluation in an Iranian population previously diagnosed with synovial sarcoma without such molecular tests.<br /> <strong> Method:</strong> We conducted this cross-sectional study on 44 formalin-fixed paraffin-embedded tissue blocks obtained from 23 synovial sarcoma patients (males, 69%; mean age, 36.4 years) and 11 cases with other neoplasms as negative controls. We assessed these specimens for SSX-SS18 gene rearrangement by break-apart fluorescent in situ hybridization (FISH) probes.<br /> <strong>Results:</strong> FISH study showed SS18-SSX fusion gene in 17 (73.91%) cases while six (26.09%) cases and negative controls did not show SS18-SSX fusion. Histopathologic type of tumor was significantly related to the presence of rearrangement (P=0.002) (rearranged: 11 biphasic and six monophasic tumors; non-rearranged: three monophasic and three poorly-differentiated tumors).<br /> <strong>Conclusion:</strong> This study supports the idea that molecular studies contribute to the confirmation of synovial sarcoma diagnosis, particularly in monophasic and poorly-differentiated subtypes, which show vague immunohistochemical results.https://mejc.sums.ac.ir/article_46787_039552932907cbbeea16d22f5c3c36d4.pdfsynovial sarcomagene fusionfluorescent in situ hybridizationss18-ssx1 fusion protein |
| spellingShingle | Akbar Safaei Elham Jansar Ahmad Monabati Maral Mokhtari Mehdi Montazer SS18-SSX Rearrangement in Synovial Sarcoma Using Fluorescent in Situ Hybridization: A Report from an Iranian Population and Literature Review Middle East Journal of Cancer synovial sarcoma gene fusion fluorescent in situ hybridization ss18-ssx1 fusion protein |
| title | SS18-SSX Rearrangement in Synovial Sarcoma Using Fluorescent in Situ Hybridization: A Report from an Iranian Population and Literature Review |
| title_full | SS18-SSX Rearrangement in Synovial Sarcoma Using Fluorescent in Situ Hybridization: A Report from an Iranian Population and Literature Review |
| title_fullStr | SS18-SSX Rearrangement in Synovial Sarcoma Using Fluorescent in Situ Hybridization: A Report from an Iranian Population and Literature Review |
| title_full_unstemmed | SS18-SSX Rearrangement in Synovial Sarcoma Using Fluorescent in Situ Hybridization: A Report from an Iranian Population and Literature Review |
| title_short | SS18-SSX Rearrangement in Synovial Sarcoma Using Fluorescent in Situ Hybridization: A Report from an Iranian Population and Literature Review |
| title_sort | ss18 ssx rearrangement in synovial sarcoma using fluorescent in situ hybridization a report from an iranian population and literature review |
| topic | synovial sarcoma gene fusion fluorescent in situ hybridization ss18-ssx1 fusion protein |
| url | https://mejc.sums.ac.ir/article_46787_039552932907cbbeea16d22f5c3c36d4.pdf |
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