Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competence
IntroductionAcquisition of germinal vesicle (GV) stage oocytes for fertility preservation (FP) offers several benefits over in vivo matured oocyte cryopreservation following ovarian stimulation, particularly for cancer patients necessitating immediate treatment. Two FP approaches for GV oocytes are...
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1200051/full |
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| author | Dongmei Deng Dongmei Deng Juan Xie Juan Xie Yin Tian Yin Tian Ling Zhu Ling Zhu Xuemei Liu Xuemei Liu Junxia Liu Junxia Liu Guoning Huang Guoning Huang Jingyu Li Jingyu Li |
| author_facet | Dongmei Deng Dongmei Deng Juan Xie Juan Xie Yin Tian Yin Tian Ling Zhu Ling Zhu Xuemei Liu Xuemei Liu Junxia Liu Junxia Liu Guoning Huang Guoning Huang Jingyu Li Jingyu Li |
| author_sort | Dongmei Deng |
| collection | DOAJ |
| description | IntroductionAcquisition of germinal vesicle (GV) stage oocytes for fertility preservation (FP) offers several benefits over in vivo matured oocyte cryopreservation following ovarian stimulation, particularly for cancer patients necessitating immediate treatment. Two FP approaches for GV oocytes are available: vitrification before in vitro maturation (IVM) at the GV stage (GV-VI) or post-IVM at the metaphase II (MII) stage (MII-VI). The optimal method remains to be determined.MethodsIn this study, mouse oocytes were collected without hormonal stimulation and vitrified either at the GV stage or the MII stage following IVM; non-vitrified in vitro matured MII oocytes served as the control (CON). The oocyte quality and developmental competence were assessed to obtain a better method for immediate FP.ResultsNo significant differences in IVM and survival rates were observed among the three groups. Nevertheless, GV-VI oocytes exhibited inferior quality, including abnormal spindle arrangement, mitochondrial dysfunction, and early apoptosis, compared to MII-VI and CON oocytes. Oocyte vitrification at the GV stage impacted maternal mRNA degradation during IVM. In addition, the GV-VI group demonstrated significantly lower embryonic developmental competence relative to the MII-VI group. RNA sequencing of 2-cell stage embryos revealed abnormal minor zygotic genome activation in the GV-VI group.ConclusionVitrification at the GV stage compromised oocyte quality and reduced developmental competence. Consequently, compared to the GV stage, oocyte vitrification at the MII stage after IVM is more suitable for patients who require immediate FP. |
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| language | English |
| last_indexed | 2024-03-13T02:50:37Z |
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| series | Frontiers in Endocrinology |
| spelling | doaj.art-9102e50d3c48411a9499cb7f4313aba22023-06-28T13:14:24ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-06-011410.3389/fendo.2023.12000511200051Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competenceDongmei Deng0Dongmei Deng1Juan Xie2Juan Xie3Yin Tian4Yin Tian5Ling Zhu6Ling Zhu7Xuemei Liu8Xuemei Liu9Junxia Liu10Junxia Liu11Guoning Huang12Guoning Huang13Jingyu Li14Jingyu Li15Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, ChinaChongqing Clinical Research Center for Reproductive Medicine, Center for Reproductive Medicine, Women and Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, ChinaChongqing Clinical Research Center for Reproductive Medicine, Center for Reproductive Medicine, Women and Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, ChinaChongqing Clinical Research Center for Reproductive Medicine, Center for Reproductive Medicine, Women and Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, ChinaChongqing Clinical Research Center for Reproductive Medicine, Center for Reproductive Medicine, Women and Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, ChinaChongqing Clinical Research Center for Reproductive Medicine, Center for Reproductive Medicine, Women and Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, ChinaChongqing Clinical Research Center for Reproductive Medicine, Center for Reproductive Medicine, Women and Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, ChinaChongqing Clinical Research Center for Reproductive Medicine, Center for Reproductive Medicine, Women and Children’s Hospital of Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, ChinaChongqing Clinical Research Center for Reproductive Medicine, Center for Reproductive Medicine, Women and Children’s Hospital of Chongqing Medical University, Chongqing, ChinaIntroductionAcquisition of germinal vesicle (GV) stage oocytes for fertility preservation (FP) offers several benefits over in vivo matured oocyte cryopreservation following ovarian stimulation, particularly for cancer patients necessitating immediate treatment. Two FP approaches for GV oocytes are available: vitrification before in vitro maturation (IVM) at the GV stage (GV-VI) or post-IVM at the metaphase II (MII) stage (MII-VI). The optimal method remains to be determined.MethodsIn this study, mouse oocytes were collected without hormonal stimulation and vitrified either at the GV stage or the MII stage following IVM; non-vitrified in vitro matured MII oocytes served as the control (CON). The oocyte quality and developmental competence were assessed to obtain a better method for immediate FP.ResultsNo significant differences in IVM and survival rates were observed among the three groups. Nevertheless, GV-VI oocytes exhibited inferior quality, including abnormal spindle arrangement, mitochondrial dysfunction, and early apoptosis, compared to MII-VI and CON oocytes. Oocyte vitrification at the GV stage impacted maternal mRNA degradation during IVM. In addition, the GV-VI group demonstrated significantly lower embryonic developmental competence relative to the MII-VI group. RNA sequencing of 2-cell stage embryos revealed abnormal minor zygotic genome activation in the GV-VI group.ConclusionVitrification at the GV stage compromised oocyte quality and reduced developmental competence. Consequently, compared to the GV stage, oocyte vitrification at the MII stage after IVM is more suitable for patients who require immediate FP.https://www.frontiersin.org/articles/10.3389/fendo.2023.1200051/fullgerminal vesicle oocyteoocyte vitrificationfertility preservationIn vitro maturationdevelopmental competence |
| spellingShingle | Dongmei Deng Dongmei Deng Juan Xie Juan Xie Yin Tian Yin Tian Ling Zhu Ling Zhu Xuemei Liu Xuemei Liu Junxia Liu Junxia Liu Guoning Huang Guoning Huang Jingyu Li Jingyu Li Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competence Frontiers in Endocrinology germinal vesicle oocyte oocyte vitrification fertility preservation In vitro maturation developmental competence |
| title | Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competence |
| title_full | Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competence |
| title_fullStr | Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competence |
| title_full_unstemmed | Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competence |
| title_short | Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competence |
| title_sort | effects of meiotic stage specific oocyte vitrification on mouse oocyte quality and developmental competence |
| topic | germinal vesicle oocyte oocyte vitrification fertility preservation In vitro maturation developmental competence |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1200051/full |
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