New Data on Cylindrospermopsin Toxicity
Cylindrospermopsin (CYN) is a widely spread cyanotoxin that can occur in fresh water and food. This research aims to investigate CYN toxicity by studying the effects of drinking 0.25 nM of CYN-contaminated water from a natural source, and of the direct application of moderate concentrations of CYN o...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/1/41 |
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| author | Mariela Chichova Oskan Tasinov Milena Shkodrova Milena Mishonova Iliyana Sazdova Bilyana Ilieva Dilyana Doncheva-Stoimenova Yoana Kiselova-Kaneva Neli Raikova Blagoy Uzunov Diana Ivanova Hristo Gagov |
| author_facet | Mariela Chichova Oskan Tasinov Milena Shkodrova Milena Mishonova Iliyana Sazdova Bilyana Ilieva Dilyana Doncheva-Stoimenova Yoana Kiselova-Kaneva Neli Raikova Blagoy Uzunov Diana Ivanova Hristo Gagov |
| author_sort | Mariela Chichova |
| collection | DOAJ |
| description | Cylindrospermopsin (CYN) is a widely spread cyanotoxin that can occur in fresh water and food. This research aims to investigate CYN toxicity by studying the effects of drinking 0.25 nM of CYN-contaminated water from a natural source, and of the direct application of moderate concentrations of CYN on different animal targets. The chosen structures and activities are rat mitochondria inner membrane permeability, mitochondrial ATP synthase (ATPase) and rat liver diamine oxidase (DAO) activities (EC 1.4.3.22.), the force of the contraction of an excised frog heart preparation with functional innervation, and the viability of a human intestinal epithelial cell line (HIEC-6). The oral exposure to CYN decreased the reverse (hydrolase) activity of rat liver ATPase whereas its short-term, in vitro application was without significant effect on this organelle, DAO activity, heart contractions, and their neuronal regulation. The application of CYN reduced HIEC-6 cells’ viability dose dependently. It was concluded that CYN is moderately toxic for the human intestinal epithelial cells, where the regeneration of the epithelial layer can be suppressed by CYN. This result suggests that CYN may provoke pathological changes in the human gastrointestinal tract. |
| first_indexed | 2024-03-09T05:36:56Z |
| format | Article |
| id | doaj.art-91036cbfd32b4abbb176f3fc54db44ba |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T05:36:56Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-91036cbfd32b4abbb176f3fc54db44ba2023-12-03T12:27:49ZengMDPI AGToxins2072-66512021-01-011314110.3390/toxins13010041New Data on Cylindrospermopsin ToxicityMariela Chichova0Oskan Tasinov1Milena Shkodrova2Milena Mishonova3Iliyana Sazdova4Bilyana Ilieva5Dilyana Doncheva-Stoimenova6Yoana Kiselova-Kaneva7Neli Raikova8Blagoy Uzunov9Diana Ivanova10Hristo Gagov11Department of Animal and Human Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaDepartment of Biochemistry, Molecular Medicine and Nutrigenomics, Faculty of Pharmacy, Medical University of Varna, 55 Marin Drinov, 9002 Varna, BulgariaDepartment of Animal and Human Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaDepartment of Animal and Human Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaDepartment of Animal and Human Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaDepartment of Animal and Human Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaDepartment of Animal and Human Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaDepartment of Biochemistry, Molecular Medicine and Nutrigenomics, Faculty of Pharmacy, Medical University of Varna, 55 Marin Drinov, 9002 Varna, BulgariaDepartment of Animal and Human Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaDepartment of Botany, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaDepartment of Biochemistry, Molecular Medicine and Nutrigenomics, Faculty of Pharmacy, Medical University of Varna, 55 Marin Drinov, 9002 Varna, BulgariaDepartment of Animal and Human Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaCylindrospermopsin (CYN) is a widely spread cyanotoxin that can occur in fresh water and food. This research aims to investigate CYN toxicity by studying the effects of drinking 0.25 nM of CYN-contaminated water from a natural source, and of the direct application of moderate concentrations of CYN on different animal targets. The chosen structures and activities are rat mitochondria inner membrane permeability, mitochondrial ATP synthase (ATPase) and rat liver diamine oxidase (DAO) activities (EC 1.4.3.22.), the force of the contraction of an excised frog heart preparation with functional innervation, and the viability of a human intestinal epithelial cell line (HIEC-6). The oral exposure to CYN decreased the reverse (hydrolase) activity of rat liver ATPase whereas its short-term, in vitro application was without significant effect on this organelle, DAO activity, heart contractions, and their neuronal regulation. The application of CYN reduced HIEC-6 cells’ viability dose dependently. It was concluded that CYN is moderately toxic for the human intestinal epithelial cells, where the regeneration of the epithelial layer can be suppressed by CYN. This result suggests that CYN may provoke pathological changes in the human gastrointestinal tract.https://www.mdpi.com/2072-6651/13/1/41cylindrospermopsinratliverheartmitochondriasemicarbazide-sensitive amine oxidase |
| spellingShingle | Mariela Chichova Oskan Tasinov Milena Shkodrova Milena Mishonova Iliyana Sazdova Bilyana Ilieva Dilyana Doncheva-Stoimenova Yoana Kiselova-Kaneva Neli Raikova Blagoy Uzunov Diana Ivanova Hristo Gagov New Data on Cylindrospermopsin Toxicity Toxins cylindrospermopsin rat liver heart mitochondria semicarbazide-sensitive amine oxidase |
| title | New Data on Cylindrospermopsin Toxicity |
| title_full | New Data on Cylindrospermopsin Toxicity |
| title_fullStr | New Data on Cylindrospermopsin Toxicity |
| title_full_unstemmed | New Data on Cylindrospermopsin Toxicity |
| title_short | New Data on Cylindrospermopsin Toxicity |
| title_sort | new data on cylindrospermopsin toxicity |
| topic | cylindrospermopsin rat liver heart mitochondria semicarbazide-sensitive amine oxidase |
| url | https://www.mdpi.com/2072-6651/13/1/41 |
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