Protective Immunity Induced by Virus-Like Particle Containing Merozoite Surface Protein 9 of <i>Plasmodium berghei</i>
Merozoite surface protein 9 (MSP-9) from <i>Plasmodium</i> has shown promise as a vaccine candidate due to its location and possible role in erythrocyte invasion. In this study, we generated virus-like particles (VLPs) targeting <i>P. berghei</i> MSP-9, and investigated the p...
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MDPI AG
2020-07-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/8/3/428 |
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| author | Su-Hwa Lee Hae-Ji Kang Ki-Back Chu Swarnendu Basak Dong-Hun Lee Eun-Kyung Moon Fu-Shi Quan |
| author_facet | Su-Hwa Lee Hae-Ji Kang Ki-Back Chu Swarnendu Basak Dong-Hun Lee Eun-Kyung Moon Fu-Shi Quan |
| author_sort | Su-Hwa Lee |
| collection | DOAJ |
| description | Merozoite surface protein 9 (MSP-9) from <i>Plasmodium</i> has shown promise as a vaccine candidate due to its location and possible role in erythrocyte invasion. In this study, we generated virus-like particles (VLPs) targeting <i>P. berghei</i> MSP-9, and investigated the protection against lethal doses of <i>P. berghei</i> in a mouse model. We found that VLP vaccination induced a <i>P. berghei</i>-specific IgG antibody response in the sera and CD4<sup>+</sup> and CD8<sup>+</sup> T cell populations in blood compared to a naïve control group. Upon challenge infection with <i>P. berghei</i>, vaccinated mice showed a significant increase in CD4<sup>+</sup> and CD8<sup>+</sup> effector memory T cell and memory B cell populations. Importantly, MSP-9 VLP immunization inhibited levels of the pro-inflammatory cytokines IFN-γ and IL-6 in the spleen and parasite replication in blood, resulting in significantly prolonged survival time. These results suggest that the MSP-9 VLP vaccine may constitute an effective malaria vaccine. |
| first_indexed | 2024-03-10T18:05:21Z |
| format | Article |
| id | doaj.art-9104a71f944d480ab9d0d1ac9dbb9223 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T18:05:21Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-9104a71f944d480ab9d0d1ac9dbb92232023-11-20T08:31:31ZengMDPI AGVaccines2076-393X2020-07-018342810.3390/vaccines8030428Protective Immunity Induced by Virus-Like Particle Containing Merozoite Surface Protein 9 of <i>Plasmodium berghei</i>Su-Hwa Lee0Hae-Ji Kang1Ki-Back Chu2Swarnendu Basak3Dong-Hun Lee4Eun-Kyung Moon5Fu-Shi Quan6Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Medical Zoology, Kyung Hee University School of Medicine, Seoul 02447, KoreaDepartment of Medical Zoology, Kyung Hee University School of Medicine, Seoul 02447, KoreaMerozoite surface protein 9 (MSP-9) from <i>Plasmodium</i> has shown promise as a vaccine candidate due to its location and possible role in erythrocyte invasion. In this study, we generated virus-like particles (VLPs) targeting <i>P. berghei</i> MSP-9, and investigated the protection against lethal doses of <i>P. berghei</i> in a mouse model. We found that VLP vaccination induced a <i>P. berghei</i>-specific IgG antibody response in the sera and CD4<sup>+</sup> and CD8<sup>+</sup> T cell populations in blood compared to a naïve control group. Upon challenge infection with <i>P. berghei</i>, vaccinated mice showed a significant increase in CD4<sup>+</sup> and CD8<sup>+</sup> effector memory T cell and memory B cell populations. Importantly, MSP-9 VLP immunization inhibited levels of the pro-inflammatory cytokines IFN-γ and IL-6 in the spleen and parasite replication in blood, resulting in significantly prolonged survival time. These results suggest that the MSP-9 VLP vaccine may constitute an effective malaria vaccine.https://www.mdpi.com/2076-393X/8/3/428<i>Plasmodium berghei</i>merozoite surface protein 9vaccinevirus-like particle |
| spellingShingle | Su-Hwa Lee Hae-Ji Kang Ki-Back Chu Swarnendu Basak Dong-Hun Lee Eun-Kyung Moon Fu-Shi Quan Protective Immunity Induced by Virus-Like Particle Containing Merozoite Surface Protein 9 of <i>Plasmodium berghei</i> Vaccines <i>Plasmodium berghei</i> merozoite surface protein 9 vaccine virus-like particle |
| title | Protective Immunity Induced by Virus-Like Particle Containing Merozoite Surface Protein 9 of <i>Plasmodium berghei</i> |
| title_full | Protective Immunity Induced by Virus-Like Particle Containing Merozoite Surface Protein 9 of <i>Plasmodium berghei</i> |
| title_fullStr | Protective Immunity Induced by Virus-Like Particle Containing Merozoite Surface Protein 9 of <i>Plasmodium berghei</i> |
| title_full_unstemmed | Protective Immunity Induced by Virus-Like Particle Containing Merozoite Surface Protein 9 of <i>Plasmodium berghei</i> |
| title_short | Protective Immunity Induced by Virus-Like Particle Containing Merozoite Surface Protein 9 of <i>Plasmodium berghei</i> |
| title_sort | protective immunity induced by virus like particle containing merozoite surface protein 9 of i plasmodium berghei i |
| topic | <i>Plasmodium berghei</i> merozoite surface protein 9 vaccine virus-like particle |
| url | https://www.mdpi.com/2076-393X/8/3/428 |
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