Association Study Between APOE and TNF- α Gene Variations and Sporadic Alzheimer’s Disease in Iranian Population
Objectives: Several studies indicate that amyloid ß (Aß) peptide deposits and neurofibrillary tangles have key roles in pathogenesis and progression of the late-onset Alzhimer’s disease. Likewise it has been shown that inflammatory reactions play a significant role as w...
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| Format: | Article |
| Language: | fas |
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University of Social Welfare & Rehabilitation Sciences
2012-10-01
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| Series: | Sālmand |
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| Online Access: | http://salmandj.uswr.ac.ir/browse.php?a_code=A-10-305-1&slc_lang=en&sid=1 |
| _version_ | 1797705860817879040 |
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| author | Niloufar Safavi Naini Korush Kamali Shideh Momeni Masoud Karimloo Mina Ohadi Mahdi Manoochehri Hamid Reza Khorram Khorshid |
| author_facet | Niloufar Safavi Naini Korush Kamali Shideh Momeni Masoud Karimloo Mina Ohadi Mahdi Manoochehri Hamid Reza Khorram Khorshid |
| author_sort | Niloufar Safavi Naini |
| collection | DOAJ |
| description | Objectives: Several studies indicate that amyloid ß (Aß) peptide deposits and neurofibrillary tangles have key roles in pathogenesis and progression of the late-onset Alzhimer’s disease. Likewise it has been shown that inflammatory reactions play a significant role as well. Inflammatory mediators such as complement, chemokines and cytokines activators and inhibitors can release from activated microglia and astrocytes, causing neuronal dysfunction and death. One of the most important cytokines is tumor necrosis factor–α (TNF-α). This study was designed and carried out to determine the association between sporadic Alzheimer’s disease and the human TNF-α and APOE gene variations in Iranian population.
Methods & Materials: In this case-control study, the role of TNF-α gene polymorphism was determined in 167 sporadic AD patients and 163 healthy controls. Genomic DNA was extracted using standard salting out method from peripheral lymphocytes and TNF-α- 850C/T promoter polymorphism was genotyped using PCR/RFLP technique. Comparing the genotype and allelic frequencies were analyzed using chi-square and logistic regression tests by SPSS 11.5.
Results: The obtained results indicated that the frequency of TNF-α -850 heterozygote genotype (CT) was significantly higher in AD patients comparing healthy controls (P=0.038). Although no significant difference were observed in TNF-α -850 homozygote genotype (TT) and T allele between the studied groups. No interaction was shown between TNF-α -850 and APOE gene polymorphisms as well.
Conclusion: These data suggests the role of TNF-α -850 TC genotype as a risk factor for AD in Iranian population. Although to show the effects of homozygote genotype (TT) and T allele, a study with a larger sample size maybe indicated. |
| first_indexed | 2024-03-12T05:43:39Z |
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| id | doaj.art-9106d76a976a4d49927968cc26ff380c |
| institution | Directory Open Access Journal |
| issn | 1735-806X 1735-806X |
| language | fas |
| last_indexed | 2024-03-12T05:43:39Z |
| publishDate | 2012-10-01 |
| publisher | University of Social Welfare & Rehabilitation Sciences |
| record_format | Article |
| series | Sālmand |
| spelling | doaj.art-9106d76a976a4d49927968cc26ff380c2023-09-03T05:51:42ZfasUniversity of Social Welfare & Rehabilitation SciencesSālmand1735-806X1735-806X2012-10-0173711Association Study Between APOE and TNF- α Gene Variations and Sporadic Alzheimer’s Disease in Iranian PopulationNiloufar Safavi Naini0Korush Kamali1Shideh Momeni2Masoud Karimloo3Mina Ohadi4Mahdi Manoochehri5Hamid Reza Khorram Khorshid6 University of Social Welfare and Rehabilitation Sciences, Tehran, Iran Genetic Research Centre, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran Reproductive Biotechnology Research Centre, Avicenna Research Institute (ACECR), Tehran, Iran University of Social Welfare and Rehabilitation Sciences, Tehran, Iran University of Social Welfare and Rehabilitation Sciences, Tehran, Iran University of Social Welfare and Rehabilitation Sciences, Tehran, Iran University of Social Welfare and Rehabilitation Sciences, Tehran, Iran Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran. Objectives: Several studies indicate that amyloid ß (Aß) peptide deposits and neurofibrillary tangles have key roles in pathogenesis and progression of the late-onset Alzhimer’s disease. Likewise it has been shown that inflammatory reactions play a significant role as well. Inflammatory mediators such as complement, chemokines and cytokines activators and inhibitors can release from activated microglia and astrocytes, causing neuronal dysfunction and death. One of the most important cytokines is tumor necrosis factor–α (TNF-α). This study was designed and carried out to determine the association between sporadic Alzheimer’s disease and the human TNF-α and APOE gene variations in Iranian population. Methods & Materials: In this case-control study, the role of TNF-α gene polymorphism was determined in 167 sporadic AD patients and 163 healthy controls. Genomic DNA was extracted using standard salting out method from peripheral lymphocytes and TNF-α- 850C/T promoter polymorphism was genotyped using PCR/RFLP technique. Comparing the genotype and allelic frequencies were analyzed using chi-square and logistic regression tests by SPSS 11.5. Results: The obtained results indicated that the frequency of TNF-α -850 heterozygote genotype (CT) was significantly higher in AD patients comparing healthy controls (P=0.038). Although no significant difference were observed in TNF-α -850 homozygote genotype (TT) and T allele between the studied groups. No interaction was shown between TNF-α -850 and APOE gene polymorphisms as well. Conclusion: These data suggests the role of TNF-α -850 TC genotype as a risk factor for AD in Iranian population. Although to show the effects of homozygote genotype (TT) and T allele, a study with a larger sample size maybe indicated.http://salmandj.uswr.ac.ir/browse.php?a_code=A-10-305-1&slc_lang=en&sid=1 Alzheimer’s disease Polymorphism TNF-α gene Case-control study |
| spellingShingle | Niloufar Safavi Naini Korush Kamali Shideh Momeni Masoud Karimloo Mina Ohadi Mahdi Manoochehri Hamid Reza Khorram Khorshid Association Study Between APOE and TNF- α Gene Variations and Sporadic Alzheimer’s Disease in Iranian Population Sālmand Alzheimer’s disease Polymorphism TNF-α gene Case-control study |
| title | Association Study Between APOE and TNF- α Gene Variations and Sporadic Alzheimer’s Disease in Iranian Population |
| title_full | Association Study Between APOE and TNF- α Gene Variations and Sporadic Alzheimer’s Disease in Iranian Population |
| title_fullStr | Association Study Between APOE and TNF- α Gene Variations and Sporadic Alzheimer’s Disease in Iranian Population |
| title_full_unstemmed | Association Study Between APOE and TNF- α Gene Variations and Sporadic Alzheimer’s Disease in Iranian Population |
| title_short | Association Study Between APOE and TNF- α Gene Variations and Sporadic Alzheimer’s Disease in Iranian Population |
| title_sort | association study between apoe and tnf α gene variations and sporadic alzheimer s disease in iranian population |
| topic | Alzheimer’s disease Polymorphism TNF-α gene Case-control study |
| url | http://salmandj.uswr.ac.ir/browse.php?a_code=A-10-305-1&slc_lang=en&sid=1 |
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