Expression profiles of catalase gene in common carp exposed to ammonia
Ammonia is one of the most harmful water quality parameters restricting the growth and survival of aquatic living organisms. As a result, fish must adapt to this stressor by modifying physiological processes that are governed by gene expression regulation. The aim of the present study was to investi...
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| Format: | Article |
| Language: | English |
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South Valley University
2022-12-01
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| Series: | SVU-International Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://svu.journals.ekb.eg/article_275749.html |
| _version_ | 1797799372677709824 |
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| author | Mahmoud Nasr Ahmad A.A. Elkamel Mohammed Youssef Walaa F.A. Emeish |
| author_facet | Mahmoud Nasr Ahmad A.A. Elkamel Mohammed Youssef Walaa F.A. Emeish |
| author_sort | Mahmoud Nasr |
| collection | DOAJ |
| description | Ammonia is one of the most harmful water quality parameters restricting the growth and survival of aquatic living organisms. As a result, fish must adapt to this stressor by modifying physiological processes that are governed by gene expression regulation. The aim of the present study was to investigate the expression profiles of antioxidant related gene, catalase (CAT) in common carp (Cyprinus carpio) fingerlings after exposure to 0.7 mg/l of unionized ammonia (UIA) in water. The relative gene expression was measured in liver, gills, and brain tissues at four time points (12 h, 2 d, 4 d, and 7 d post exposure). The expression level of CAT gene in the liver and brain peaked after 7 d of ammonia exposure by 13.3 and 5.2-folds, respectively, but in gills it upregulated only after 2 d (2.7-folds) and downregulated at the other time points. This study proved that exposure to ammonia affects the antioxidant status of common carp as indicated by the altered levels of expression of CAT gene. |
| first_indexed | 2024-03-13T04:17:54Z |
| format | Article |
| id | doaj.art-91098fabd87846afa0ae761fd1648420 |
| institution | Directory Open Access Journal |
| issn | 2535-1826 2535-1877 |
| language | English |
| last_indexed | 2024-03-13T04:17:54Z |
| publishDate | 2022-12-01 |
| publisher | South Valley University |
| record_format | Article |
| series | SVU-International Journal of Veterinary Sciences |
| spelling | doaj.art-91098fabd87846afa0ae761fd16484202023-06-20T18:16:51ZengSouth Valley UniversitySVU-International Journal of Veterinary Sciences2535-18262535-18772022-12-015411412410.21608/SVU.2022.155871.1223Expression profiles of catalase gene in common carp exposed to ammoniaMahmoud Nasr0Ahmad A.A. Elkamel1Mohammed Youssef 2Walaa F.A. Emeish3Department of Fish Diseases, Faculty of Veterinary Medicine, South Valley University, Qena 83523, EgyptDepartment of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Assiut UniversityDepartment of Animal physiology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, EgyptDepartment of Fish Diseases, Faculty of Veterinary Medicine, South Valley University, Qena 83523, EgyptAmmonia is one of the most harmful water quality parameters restricting the growth and survival of aquatic living organisms. As a result, fish must adapt to this stressor by modifying physiological processes that are governed by gene expression regulation. The aim of the present study was to investigate the expression profiles of antioxidant related gene, catalase (CAT) in common carp (Cyprinus carpio) fingerlings after exposure to 0.7 mg/l of unionized ammonia (UIA) in water. The relative gene expression was measured in liver, gills, and brain tissues at four time points (12 h, 2 d, 4 d, and 7 d post exposure). The expression level of CAT gene in the liver and brain peaked after 7 d of ammonia exposure by 13.3 and 5.2-folds, respectively, but in gills it upregulated only after 2 d (2.7-folds) and downregulated at the other time points. This study proved that exposure to ammonia affects the antioxidant status of common carp as indicated by the altered levels of expression of CAT gene. https://svu.journals.ekb.eg/article_275749.htmlcommon carpammoniagene expressionantioxidant-related genescat |
| spellingShingle | Mahmoud Nasr Ahmad A.A. Elkamel Mohammed Youssef Walaa F.A. Emeish Expression profiles of catalase gene in common carp exposed to ammonia SVU-International Journal of Veterinary Sciences common carp ammonia gene expression antioxidant-related genes cat |
| title | Expression profiles of catalase gene in common carp exposed to ammonia |
| title_full | Expression profiles of catalase gene in common carp exposed to ammonia |
| title_fullStr | Expression profiles of catalase gene in common carp exposed to ammonia |
| title_full_unstemmed | Expression profiles of catalase gene in common carp exposed to ammonia |
| title_short | Expression profiles of catalase gene in common carp exposed to ammonia |
| title_sort | expression profiles of catalase gene in common carp exposed to ammonia |
| topic | common carp ammonia gene expression antioxidant-related genes cat |
| url | https://svu.journals.ekb.eg/article_275749.html |
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