Microarray analyses reveal novel targets of exercise-induced stress resistance in the dorsal raphe nucleus
Serotonin (5-HT) is implicated in the development of stress-related mood disorders in humans. Physical activity reduces the risk of developing stress-related mood disorders, such as depression and anxiety. In rats, 6 weeks of wheel running protects against stress-induced behaviors thought to resembl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-05-01
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| Series: | Frontiers in Behavioral Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnbeh.2013.00037/full |
| _version_ | 1828523752896331776 |
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| author | Alice B. Loughridge Benjamin N Greenwood Heidi E. W. Day Matthew B. McQueen Monika eFleshner |
| author_facet | Alice B. Loughridge Benjamin N Greenwood Heidi E. W. Day Matthew B. McQueen Monika eFleshner |
| author_sort | Alice B. Loughridge |
| collection | DOAJ |
| description | Serotonin (5-HT) is implicated in the development of stress-related mood disorders in humans. Physical activity reduces the risk of developing stress-related mood disorders, such as depression and anxiety. In rats, 6 weeks of wheel running protects against stress-induced behaviors thought to resemble symptoms of human anxiety and depression. The mechanisms by which exercise confers protection against stress-induced behaviors, however, remain unknown. One way by which exercise could generate stress resistance is by producing plastic changes in gene expression in the dorsal raphe nucleus (DRN). The DRN has a high concentration of 5-HT neurons and is implicated in stress-related mood disorders. The goal of the current experiment was to identify changes in the expression of genes that could be novel targets of exercise-induced stress resistance in the DRN. Adult, male F344 rats were allowed voluntary access to running wheels for 6 weeks; exposed to inescapable stress or no stress; and sacrificed immediately and 2 hours after stressor termination. Laser capture microdissection selectively sampled the DRN. mRNA expression was measured using the whole genome Affymetrix microarray. Comprehensive data analyses of gene expression included differential gene expression, log fold change (LFC) contrast analyses with False Discovery Rate correction, KEGG and Wiki Web Gestalt pathway enrichment analyses, and Weighted Gene Correlational Network Analysis (WGCNA). Our results suggest that physically active rats exposed to stress modulate expression of twice the number of genes, and display a more rapid and strongly coordinated response, than sedentary rats. Bioinformatics analyses revealed several potential targets of stress resistance including genes that are related to immune processes, tryptophan metabolism, and circadian/diurnal rhythms. |
| first_indexed | 2024-12-11T20:33:32Z |
| format | Article |
| id | doaj.art-9109c4eb10584e8694ee9e11c7d40e65 |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-12-11T20:33:32Z |
| publishDate | 2013-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-9109c4eb10584e8694ee9e11c7d40e652022-12-22T00:51:44ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532013-05-01710.3389/fnbeh.2013.0003743800Microarray analyses reveal novel targets of exercise-induced stress resistance in the dorsal raphe nucleusAlice B. Loughridge0Benjamin N Greenwood1Heidi E. W. Day2Matthew B. McQueen3Monika eFleshner4University of ColoradoUniversity of ColoradoUniversity of ColoradoUniversity of ColoradoUniversity of ColoradoSerotonin (5-HT) is implicated in the development of stress-related mood disorders in humans. Physical activity reduces the risk of developing stress-related mood disorders, such as depression and anxiety. In rats, 6 weeks of wheel running protects against stress-induced behaviors thought to resemble symptoms of human anxiety and depression. The mechanisms by which exercise confers protection against stress-induced behaviors, however, remain unknown. One way by which exercise could generate stress resistance is by producing plastic changes in gene expression in the dorsal raphe nucleus (DRN). The DRN has a high concentration of 5-HT neurons and is implicated in stress-related mood disorders. The goal of the current experiment was to identify changes in the expression of genes that could be novel targets of exercise-induced stress resistance in the DRN. Adult, male F344 rats were allowed voluntary access to running wheels for 6 weeks; exposed to inescapable stress or no stress; and sacrificed immediately and 2 hours after stressor termination. Laser capture microdissection selectively sampled the DRN. mRNA expression was measured using the whole genome Affymetrix microarray. Comprehensive data analyses of gene expression included differential gene expression, log fold change (LFC) contrast analyses with False Discovery Rate correction, KEGG and Wiki Web Gestalt pathway enrichment analyses, and Weighted Gene Correlational Network Analysis (WGCNA). Our results suggest that physically active rats exposed to stress modulate expression of twice the number of genes, and display a more rapid and strongly coordinated response, than sedentary rats. Bioinformatics analyses revealed several potential targets of stress resistance including genes that are related to immune processes, tryptophan metabolism, and circadian/diurnal rhythms.http://journal.frontiersin.org/Journal/10.3389/fnbeh.2013.00037/fullLaser Capture Microdissectionbioinformaticsdorsal raphe nucleusStress resistanceAffymetrix gene microarrayweighted gene correlational network analysis |
| spellingShingle | Alice B. Loughridge Benjamin N Greenwood Heidi E. W. Day Matthew B. McQueen Monika eFleshner Microarray analyses reveal novel targets of exercise-induced stress resistance in the dorsal raphe nucleus Frontiers in Behavioral Neuroscience Laser Capture Microdissection bioinformatics dorsal raphe nucleus Stress resistance Affymetrix gene microarray weighted gene correlational network analysis |
| title | Microarray analyses reveal novel targets of exercise-induced stress resistance in the dorsal raphe nucleus |
| title_full | Microarray analyses reveal novel targets of exercise-induced stress resistance in the dorsal raphe nucleus |
| title_fullStr | Microarray analyses reveal novel targets of exercise-induced stress resistance in the dorsal raphe nucleus |
| title_full_unstemmed | Microarray analyses reveal novel targets of exercise-induced stress resistance in the dorsal raphe nucleus |
| title_short | Microarray analyses reveal novel targets of exercise-induced stress resistance in the dorsal raphe nucleus |
| title_sort | microarray analyses reveal novel targets of exercise induced stress resistance in the dorsal raphe nucleus |
| topic | Laser Capture Microdissection bioinformatics dorsal raphe nucleus Stress resistance Affymetrix gene microarray weighted gene correlational network analysis |
| url | http://journal.frontiersin.org/Journal/10.3389/fnbeh.2013.00037/full |
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