Genetic survey of alveolar and cystic echinococcoses in Romania: first molecular evidence of Echinococcus multilocularis in humans in the country
Cystic echinococcosis (CE) and alveolar echinococcosis (AE) are considered as one of the most important zoonotic diseases in Romania, where they are subject to mandatory reporting. To obtain more knowledge about the genetic diversity of Echinococcus causative agents of these diseases, 11 isolates fr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2017-09-01
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| Series: | Helminthologia |
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| Online Access: | https://doi.org/10.1515/helm-2017-0025 |
| _version_ | 1827840105761472512 |
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| author | Šnábel V. Calma C. Georgescu S. O. Cavallero S. D’Amelio S. Vasilková Z. Ito A. |
| author_facet | Šnábel V. Calma C. Georgescu S. O. Cavallero S. D’Amelio S. Vasilková Z. Ito A. |
| author_sort | Šnábel V. |
| collection | DOAJ |
| description | Cystic echinococcosis (CE) and alveolar echinococcosis (AE) are considered as one of the most important zoonotic diseases in Romania, where they are subject to mandatory reporting. To obtain more knowledge about the genetic diversity of Echinococcus causative agents of these diseases, 11 isolates from humans and ungulate intermediate hosts from the two regions of Romania were genotyped using mitochondrial markers. In clinical samples of five patients from north-eastern Romania (Iasi, Botosani, Vaslui counties), Echinococcus multilocularis was identified as causal agent by cox1 sequence analysis. To the best of our knowledge this finding presents the first molecular evidence of E. multilocularis in humans from Romania. Only two cases of AE in patients were previously documented in the country by serological methods. In our four patients the most widespread European variant E5 of E. multilocularis was recorded, whereas in isolate from Vaslui county three nucleotide substitutions were detected as compared to the most related E5 haplotype. One of these mutations (411T/G) matched N1 and N2 haplotypes described previously from North America. In six CE samples retrieved from western Romania (Caras-Severin and Timis counties), two human isolates were diagnosed as Echinococcus canadensis G7, one as E. granulosus s.s. G1 and one as E. granulosus s.s. G3 using atp6 and rrnS sequencing. In ungulates, the cattle isolate was allocated to E. granulosus s.s. G1 and pig isolate to E. canadensis G7. The two G7 findings in humans reinforced the recent view that G7 was underestimated as compared to the E. granulosus s.s. regarding human CE threat that can be further employed for identifying sources of infections and establishing suitable preventive measures. |
| first_indexed | 2024-03-12T07:29:21Z |
| format | Article |
| id | doaj.art-91104d6543cf4c469899e24271f240f7 |
| institution | Directory Open Access Journal |
| issn | 0440-6605 1336-9083 |
| language | English |
| last_indexed | 2024-03-12T07:29:21Z |
| publishDate | 2017-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Helminthologia |
| spelling | doaj.art-91104d6543cf4c469899e24271f240f72023-09-02T21:53:42ZengSciendoHelminthologia0440-66051336-90832017-09-0154318919810.1515/helm-2017-0025helm-2017-0025Genetic survey of alveolar and cystic echinococcoses in Romania: first molecular evidence of Echinococcus multilocularis in humans in the countryŠnábel V.0Calma C.1Georgescu S. O.2Cavallero S.3D’Amelio S.4Vasilková Z.5Ito A.6Institute of Parasitology, Slovak Academy of Sciences, Košice, Hlinkova 3, 04001Košice, SlovakiaVictor Babes University of Medicine and Pharmacy, Department of Physiology and Immunology, 300041Timisoara, RomaniaGrigore T. Popa University of Medicine and Pharmacy, Department of Surgery, Iași, RomaniaDepartment of Public Health and Infectious Diseases, Section of Parasitology, Sapienza University of Rome, Rome, ItalyDepartment of Public Health and Infectious Diseases, Section of Parasitology, Sapienza University of Rome, Rome, ItalyInstitute of Parasitology, Slovak Academy of Sciences, Košice, Hlinkova 3, 04001Košice, SlovakiaDepartment of Parasitology, Asahikawa Medical University, Asahikawa078-8510, JapanCystic echinococcosis (CE) and alveolar echinococcosis (AE) are considered as one of the most important zoonotic diseases in Romania, where they are subject to mandatory reporting. To obtain more knowledge about the genetic diversity of Echinococcus causative agents of these diseases, 11 isolates from humans and ungulate intermediate hosts from the two regions of Romania were genotyped using mitochondrial markers. In clinical samples of five patients from north-eastern Romania (Iasi, Botosani, Vaslui counties), Echinococcus multilocularis was identified as causal agent by cox1 sequence analysis. To the best of our knowledge this finding presents the first molecular evidence of E. multilocularis in humans from Romania. Only two cases of AE in patients were previously documented in the country by serological methods. In our four patients the most widespread European variant E5 of E. multilocularis was recorded, whereas in isolate from Vaslui county three nucleotide substitutions were detected as compared to the most related E5 haplotype. One of these mutations (411T/G) matched N1 and N2 haplotypes described previously from North America. In six CE samples retrieved from western Romania (Caras-Severin and Timis counties), two human isolates were diagnosed as Echinococcus canadensis G7, one as E. granulosus s.s. G1 and one as E. granulosus s.s. G3 using atp6 and rrnS sequencing. In ungulates, the cattle isolate was allocated to E. granulosus s.s. G1 and pig isolate to E. canadensis G7. The two G7 findings in humans reinforced the recent view that G7 was underestimated as compared to the E. granulosus s.s. regarding human CE threat that can be further employed for identifying sources of infections and establishing suitable preventive measures.https://doi.org/10.1515/helm-2017-0025echinococcusromaniagenotypehumancattlepig |
| spellingShingle | Šnábel V. Calma C. Georgescu S. O. Cavallero S. D’Amelio S. Vasilková Z. Ito A. Genetic survey of alveolar and cystic echinococcoses in Romania: first molecular evidence of Echinococcus multilocularis in humans in the country Helminthologia echinococcus romania genotype human cattle pig |
| title | Genetic survey of alveolar and cystic echinococcoses in Romania: first molecular evidence of Echinococcus multilocularis in humans in the country |
| title_full | Genetic survey of alveolar and cystic echinococcoses in Romania: first molecular evidence of Echinococcus multilocularis in humans in the country |
| title_fullStr | Genetic survey of alveolar and cystic echinococcoses in Romania: first molecular evidence of Echinococcus multilocularis in humans in the country |
| title_full_unstemmed | Genetic survey of alveolar and cystic echinococcoses in Romania: first molecular evidence of Echinococcus multilocularis in humans in the country |
| title_short | Genetic survey of alveolar and cystic echinococcoses in Romania: first molecular evidence of Echinococcus multilocularis in humans in the country |
| title_sort | genetic survey of alveolar and cystic echinococcoses in romania first molecular evidence of echinococcus multilocularis in humans in the country |
| topic | echinococcus romania genotype human cattle pig |
| url | https://doi.org/10.1515/helm-2017-0025 |
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