Elevated serum levels of soluble CD154 in children with juvenile idiopathic arthritis
<p>Abstract</p> <p>Objective</p> <p>Cytokines play important roles in mediating inflammation in autoimmunity. Several cytokines are elevated in serum and synovial fluid samples from children with Juvenile Idiopathic Arthritis (JIA). Soluble CD154 (sCD154) is elevated in...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-05-01
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| Series: | Pediatric Rheumatology Online Journal |
| Online Access: | http://www.ped-rheum.com/content/6/1/8 |
| _version_ | 1819028686508130304 |
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| author | Zeft Andrew S Clifford Bronte Whiting April Tebo Anne E Martins Thomas B Prahalad Sampath McNally Bernadette Bohnsack John F Hill Harry R |
| author_facet | Zeft Andrew S Clifford Bronte Whiting April Tebo Anne E Martins Thomas B Prahalad Sampath McNally Bernadette Bohnsack John F Hill Harry R |
| author_sort | Zeft Andrew S |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Objective</p> <p>Cytokines play important roles in mediating inflammation in autoimmunity. Several cytokines are elevated in serum and synovial fluid samples from children with Juvenile Idiopathic Arthritis (JIA). Soluble CD154 (sCD154) is elevated in other autoimmune disorders, but has not been characterized in JIA. Our objectives were to determine if sCD154 is elevated in JIA, and to examine correlations between sCD154 and other inflammatory cytokines.</p> <p>Methods</p> <p>Serum from 77 children with JIA and 81 pediatric controls was analyzed for interleukin (IL)1β, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, sCD154, interferon-γ (IFNγ), soluble IL2 receptor (sIL2R), and tumor necrosis factor-α (TNFα), using the Luminex Multi-Analyte Profiling system. Differences in levels of cytokines between cases and controls were analyzed. Logistic regression was also performed.</p> <p>Results</p> <p>sCD154 was significantly elevated in cases compared to controls (p < 0.0001). IL1β, IL5, IL6, IL8, IL13, IFNγ, sIL2R, and TNFα were also significantly elevated in JIA. Levels of sCD154 were highly correlated with IL1β, IL6, IL8, and TNFα (p < 0.0001). Logistic regression analysis suggested that IL6 (odds ratio (OR): 1.4, p < 0.0001), sCD154 (OR: 1.1, p < 0.0001), and TNFα (OR: 1.1, p < 0.005) were positively associated with JIA, while IL10 (OR: 0.5, p < 0.002) was protective. sCD154 was elevated in all JIA subtypes, with highest levels among more severe subtypes. IL1β, IL6, IL8, sIL2R and TNFα were also elevated in several JIA subtypes.</p> <p>Conclusion</p> <p>Serum levels of sCD154, IL1β, IL6, IL8, sIL2R and TNFα are elevated in most JIA subtypes, suggesting a major role for sCD154, and these cytokines and cytokine receptors in the pathogenesis of JIA.</p> |
| first_indexed | 2024-12-21T06:02:19Z |
| format | Article |
| id | doaj.art-911486ea3a8d47e8baa0c6b5f3ae8682 |
| institution | Directory Open Access Journal |
| issn | 1546-0096 |
| language | English |
| last_indexed | 2024-12-21T06:02:19Z |
| publishDate | 2008-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Pediatric Rheumatology Online Journal |
| spelling | doaj.art-911486ea3a8d47e8baa0c6b5f3ae86822022-12-21T19:13:44ZengBMCPediatric Rheumatology Online Journal1546-00962008-05-0161810.1186/1546-0096-6-8Elevated serum levels of soluble CD154 in children with juvenile idiopathic arthritisZeft Andrew SClifford BronteWhiting AprilTebo Anne EMartins Thomas BPrahalad SampathMcNally BernadetteBohnsack John FHill Harry R<p>Abstract</p> <p>Objective</p> <p>Cytokines play important roles in mediating inflammation in autoimmunity. Several cytokines are elevated in serum and synovial fluid samples from children with Juvenile Idiopathic Arthritis (JIA). Soluble CD154 (sCD154) is elevated in other autoimmune disorders, but has not been characterized in JIA. Our objectives were to determine if sCD154 is elevated in JIA, and to examine correlations between sCD154 and other inflammatory cytokines.</p> <p>Methods</p> <p>Serum from 77 children with JIA and 81 pediatric controls was analyzed for interleukin (IL)1β, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, sCD154, interferon-γ (IFNγ), soluble IL2 receptor (sIL2R), and tumor necrosis factor-α (TNFα), using the Luminex Multi-Analyte Profiling system. Differences in levels of cytokines between cases and controls were analyzed. Logistic regression was also performed.</p> <p>Results</p> <p>sCD154 was significantly elevated in cases compared to controls (p < 0.0001). IL1β, IL5, IL6, IL8, IL13, IFNγ, sIL2R, and TNFα were also significantly elevated in JIA. Levels of sCD154 were highly correlated with IL1β, IL6, IL8, and TNFα (p < 0.0001). Logistic regression analysis suggested that IL6 (odds ratio (OR): 1.4, p < 0.0001), sCD154 (OR: 1.1, p < 0.0001), and TNFα (OR: 1.1, p < 0.005) were positively associated with JIA, while IL10 (OR: 0.5, p < 0.002) was protective. sCD154 was elevated in all JIA subtypes, with highest levels among more severe subtypes. IL1β, IL6, IL8, sIL2R and TNFα were also elevated in several JIA subtypes.</p> <p>Conclusion</p> <p>Serum levels of sCD154, IL1β, IL6, IL8, sIL2R and TNFα are elevated in most JIA subtypes, suggesting a major role for sCD154, and these cytokines and cytokine receptors in the pathogenesis of JIA.</p>http://www.ped-rheum.com/content/6/1/8 |
| spellingShingle | Zeft Andrew S Clifford Bronte Whiting April Tebo Anne E Martins Thomas B Prahalad Sampath McNally Bernadette Bohnsack John F Hill Harry R Elevated serum levels of soluble CD154 in children with juvenile idiopathic arthritis Pediatric Rheumatology Online Journal |
| title | Elevated serum levels of soluble CD154 in children with juvenile idiopathic arthritis |
| title_full | Elevated serum levels of soluble CD154 in children with juvenile idiopathic arthritis |
| title_fullStr | Elevated serum levels of soluble CD154 in children with juvenile idiopathic arthritis |
| title_full_unstemmed | Elevated serum levels of soluble CD154 in children with juvenile idiopathic arthritis |
| title_short | Elevated serum levels of soluble CD154 in children with juvenile idiopathic arthritis |
| title_sort | elevated serum levels of soluble cd154 in children with juvenile idiopathic arthritis |
| url | http://www.ped-rheum.com/content/6/1/8 |
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