An Efficient and Rapid Assay for Detecting Neutralizing Antibodies Against Serotype 4 Fowl Adenovirus
Currently, the outbreak of serotype 4 fowl adenovirus (FAdV-4) has spread worldwide and caused tremendous economic loss to the poultry industry. Although inactivated vaccines have been licensed against FAdV-4 in China, a rapid and efficient serological method for measuring the titer of neutralizing...
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.867697/full |
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| author | Yiwen Guo Yiwen Guo Yiwen Guo Yiwen Guo Songhua Xie Songhua Xie Songhua Xie Songhua Xie Zhenqi Xu Zhenqi Xu Zhenqi Xu Zhenqi Xu Quan Xie Quan Xie Quan Xie Quan Xie Weikang Wang Weikang Wang Weikang Wang Weikang Wang Zhimin Wan Zhimin Wan Zhimin Wan Zhimin Wan Tuofan Li Tuofan Li Tuofan Li Tuofan Li Aijian Qin Aijian Qin Aijian Qin Aijian Qin Hongxia Shao Hongxia Shao Hongxia Shao Hongxia Shao Jianqiang Ye Jianqiang Ye Jianqiang Ye Jianqiang Ye |
| author_facet | Yiwen Guo Yiwen Guo Yiwen Guo Yiwen Guo Songhua Xie Songhua Xie Songhua Xie Songhua Xie Zhenqi Xu Zhenqi Xu Zhenqi Xu Zhenqi Xu Quan Xie Quan Xie Quan Xie Quan Xie Weikang Wang Weikang Wang Weikang Wang Weikang Wang Zhimin Wan Zhimin Wan Zhimin Wan Zhimin Wan Tuofan Li Tuofan Li Tuofan Li Tuofan Li Aijian Qin Aijian Qin Aijian Qin Aijian Qin Hongxia Shao Hongxia Shao Hongxia Shao Hongxia Shao Jianqiang Ye Jianqiang Ye Jianqiang Ye Jianqiang Ye |
| author_sort | Yiwen Guo |
| collection | DOAJ |
| description | Currently, the outbreak of serotype 4 fowl adenovirus (FAdV-4) has spread worldwide and caused tremendous economic loss to the poultry industry. Although inactivated vaccines have been licensed against FAdV-4 in China, a rapid and efficient serological method for measuring the titer of neutralizing antibodies (NAbs) specific for FAdV-4 post-infection or vaccination is rarely reported. Classical virus neutralization test (VNT) is superior in sensitivity and specificity for detecting NAbs but is either time-consuming or laborious. In this study, a recombinant virus FA4-EGFP expressing EGFP-fiber-2 fusion protein, rather than wild type (WT) FAdV-4 was used to develop a novel VNT for detecting FAdV-4 NAbs. Specificity analysis showed that the approach only reacted with the sera against FAdV-4, not with the sera against other avian pathogens tested. The novel VNT was effective in the detection of NAbs against FAdV-4 in sera from both experimentally infected and clinically vaccinated chickens, and had good linear correlation with the classical VNT. Moreover, the novel VNT not only significantly simplifies the procedure for detection of NAbs, but also shortens the timeline to 24 h in comparison with the classical VNT with 3-4 d. All these data demonstrate that the FA4-EGFP based VNT developed here provides an efficient diagnostic method for monitoring the immunological state of the vaccination or diagnosing the clinical infection of FAdV-4 in a quick and funding-saving manner. |
| first_indexed | 2024-12-21T05:04:02Z |
| format | Article |
| id | doaj.art-91177a7f31584064b5167307d90e2047 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-21T05:04:02Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-91177a7f31584064b5167307d90e20472022-12-21T19:15:10ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-04-01910.3389/fvets.2022.867697867697An Efficient and Rapid Assay for Detecting Neutralizing Antibodies Against Serotype 4 Fowl AdenovirusYiwen Guo0Yiwen Guo1Yiwen Guo2Yiwen Guo3Songhua Xie4Songhua Xie5Songhua Xie6Songhua Xie7Zhenqi Xu8Zhenqi Xu9Zhenqi Xu10Zhenqi Xu11Quan Xie12Quan Xie13Quan Xie14Quan Xie15Weikang Wang16Weikang Wang17Weikang Wang18Weikang Wang19Zhimin Wan20Zhimin Wan21Zhimin Wan22Zhimin Wan23Tuofan Li24Tuofan Li25Tuofan Li26Tuofan Li27Aijian Qin28Aijian Qin29Aijian Qin30Aijian Qin31Hongxia Shao32Hongxia Shao33Hongxia Shao34Hongxia Shao35Jianqiang Ye36Jianqiang Ye37Jianqiang Ye38Jianqiang Ye39Ministry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, ChinaMinistry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, ChinaMinistry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, ChinaMinistry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, ChinaMinistry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, ChinaMinistry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, ChinaMinistry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, ChinaMinistry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, ChinaMinistry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, ChinaMinistry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, ChinaCurrently, the outbreak of serotype 4 fowl adenovirus (FAdV-4) has spread worldwide and caused tremendous economic loss to the poultry industry. Although inactivated vaccines have been licensed against FAdV-4 in China, a rapid and efficient serological method for measuring the titer of neutralizing antibodies (NAbs) specific for FAdV-4 post-infection or vaccination is rarely reported. Classical virus neutralization test (VNT) is superior in sensitivity and specificity for detecting NAbs but is either time-consuming or laborious. In this study, a recombinant virus FA4-EGFP expressing EGFP-fiber-2 fusion protein, rather than wild type (WT) FAdV-4 was used to develop a novel VNT for detecting FAdV-4 NAbs. Specificity analysis showed that the approach only reacted with the sera against FAdV-4, not with the sera against other avian pathogens tested. The novel VNT was effective in the detection of NAbs against FAdV-4 in sera from both experimentally infected and clinically vaccinated chickens, and had good linear correlation with the classical VNT. Moreover, the novel VNT not only significantly simplifies the procedure for detection of NAbs, but also shortens the timeline to 24 h in comparison with the classical VNT with 3-4 d. All these data demonstrate that the FA4-EGFP based VNT developed here provides an efficient diagnostic method for monitoring the immunological state of the vaccination or diagnosing the clinical infection of FAdV-4 in a quick and funding-saving manner.https://www.frontiersin.org/articles/10.3389/fvets.2022.867697/fullserotype 4 fowl adenovirusvirus neutralization testrecombinant virusserological detectionhigh efficacy |
| spellingShingle | Yiwen Guo Yiwen Guo Yiwen Guo Yiwen Guo Songhua Xie Songhua Xie Songhua Xie Songhua Xie Zhenqi Xu Zhenqi Xu Zhenqi Xu Zhenqi Xu Quan Xie Quan Xie Quan Xie Quan Xie Weikang Wang Weikang Wang Weikang Wang Weikang Wang Zhimin Wan Zhimin Wan Zhimin Wan Zhimin Wan Tuofan Li Tuofan Li Tuofan Li Tuofan Li Aijian Qin Aijian Qin Aijian Qin Aijian Qin Hongxia Shao Hongxia Shao Hongxia Shao Hongxia Shao Jianqiang Ye Jianqiang Ye Jianqiang Ye Jianqiang Ye An Efficient and Rapid Assay for Detecting Neutralizing Antibodies Against Serotype 4 Fowl Adenovirus Frontiers in Veterinary Science serotype 4 fowl adenovirus virus neutralization test recombinant virus serological detection high efficacy |
| title | An Efficient and Rapid Assay for Detecting Neutralizing Antibodies Against Serotype 4 Fowl Adenovirus |
| title_full | An Efficient and Rapid Assay for Detecting Neutralizing Antibodies Against Serotype 4 Fowl Adenovirus |
| title_fullStr | An Efficient and Rapid Assay for Detecting Neutralizing Antibodies Against Serotype 4 Fowl Adenovirus |
| title_full_unstemmed | An Efficient and Rapid Assay for Detecting Neutralizing Antibodies Against Serotype 4 Fowl Adenovirus |
| title_short | An Efficient and Rapid Assay for Detecting Neutralizing Antibodies Against Serotype 4 Fowl Adenovirus |
| title_sort | efficient and rapid assay for detecting neutralizing antibodies against serotype 4 fowl adenovirus |
| topic | serotype 4 fowl adenovirus virus neutralization test recombinant virus serological detection high efficacy |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.867697/full |
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