The association between matrix metalloprotease-9 gene polymorphisms and primary angle-closure glaucoma in a Chinese Han population
<b>AIM:</b>To examine the association between the single nucleotide polymorphisms (SNPs) of matrix metalloprotease-9 <i>(MMP-9) </i>gene and primary angle-closure glaucoma (PACG) in a Chinese Han population.<b>METHODS:</b> DNA samples were extracted from periphera...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2014-06-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067648/ |
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| author | Xiao-Jin Gao Sheng-Ping Hou Ping-Hua Li |
| author_facet | Xiao-Jin Gao Sheng-Ping Hou Ping-Hua Li |
| author_sort | Xiao-Jin Gao |
| collection | DOAJ |
| description | <b>AIM:</b>To examine the association between the single nucleotide polymorphisms (SNPs) of matrix metalloprotease-9 <i>(MMP-9) </i>gene and primary angle-closure glaucoma (PACG) in a Chinese Han population.<b>METHODS:</b> DNA samples were extracted from peripheral-blood mononuclear cells of 214 PACG patients and 224 healthy controls. Genotyping of rs3918249, rs3918254, rs17577 and rs3787268 in <i>MMP-9</i> was performed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis and the direct sequencing technique. The association between these genetic polymorphisms and risk of PACG was estimated by <i>χ</i><sup>2 </sup>test.<b>RESULTS:</b> The distributions of rs3918249, rs3918254, rs17577 and rs3787268 genotypes among cases and healthy controls were compatible with that from Hardy-Weinberg equilibrium (HWE, <i>P></i>0.05).The increased frequency of CC and CT genotypes of rs3918254 were observed in PACG patients compared to healthy controls [<i>P=</i>0.006, <i>P</i>corrected (Pcorr)=0.048]. The haplotype analysis showed that the CCGG haplotype was nominal associated with PACG (<i>P=</i>0.015), however, the significant was lost when the Bonferroni correction was used (Pcorr=0.105).<b>CONCLUSION:</b>Our results revealed that rs3918254 in <i>MMP-9</i> may be a susceptible locus to PACG in China, people with the CC and CT genotypes of rs3918254 are more susceptible to PACG. The susceptibility to PACG in Chinese Han patients may be not influenced by SNPs rs3918249, rs3787268 and rs17577 in <i>MMP-9</i>. |
| first_indexed | 2024-12-23T05:41:40Z |
| format | Article |
| id | doaj.art-91198fac86a142bcbb9ff1b6306a0c3f |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-23T05:41:40Z |
| publishDate | 2014-06-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-91198fac86a142bcbb9ff1b6306a0c3f2022-12-21T17:58:10ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982014-06-017339740210.3980/j.issn.2222-3959.2014.03.02The association between matrix metalloprotease-9 gene polymorphisms and primary angle-closure glaucoma in a Chinese Han populationXiao-Jin Gao0Sheng-Ping Hou1Ping-Hua Li2Department of Ophthalmology, Chengdu First People’s Hospital, Chengdu 610017, Sichuan Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China<br>;Chongqing Key Laboratory of Ophthalmology, Chongqing 400016, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China<br>;Chongqing Key Laboratory of Ophthalmology, Chongqing 400016, China<b>AIM:</b>To examine the association between the single nucleotide polymorphisms (SNPs) of matrix metalloprotease-9 <i>(MMP-9) </i>gene and primary angle-closure glaucoma (PACG) in a Chinese Han population.<b>METHODS:</b> DNA samples were extracted from peripheral-blood mononuclear cells of 214 PACG patients and 224 healthy controls. Genotyping of rs3918249, rs3918254, rs17577 and rs3787268 in <i>MMP-9</i> was performed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis and the direct sequencing technique. The association between these genetic polymorphisms and risk of PACG was estimated by <i>χ</i><sup>2 </sup>test.<b>RESULTS:</b> The distributions of rs3918249, rs3918254, rs17577 and rs3787268 genotypes among cases and healthy controls were compatible with that from Hardy-Weinberg equilibrium (HWE, <i>P></i>0.05).The increased frequency of CC and CT genotypes of rs3918254 were observed in PACG patients compared to healthy controls [<i>P=</i>0.006, <i>P</i>corrected (Pcorr)=0.048]. The haplotype analysis showed that the CCGG haplotype was nominal associated with PACG (<i>P=</i>0.015), however, the significant was lost when the Bonferroni correction was used (Pcorr=0.105).<b>CONCLUSION:</b>Our results revealed that rs3918254 in <i>MMP-9</i> may be a susceptible locus to PACG in China, people with the CC and CT genotypes of rs3918254 are more susceptible to PACG. The susceptibility to PACG in Chinese Han patients may be not influenced by SNPs rs3918249, rs3787268 and rs17577 in <i>MMP-9</i>.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067648/matrix metalloprotease-9 geneprimary angle-closure glaucomasingle nucleotide polymorphismssingle nucleotide polymorphisms |
| spellingShingle | Xiao-Jin Gao Sheng-Ping Hou Ping-Hua Li The association between matrix metalloprotease-9 gene polymorphisms and primary angle-closure glaucoma in a Chinese Han population International Journal of Ophthalmology matrix metalloprotease-9 gene primary angle-closure glaucoma single nucleotide polymorphisms single nucleotide polymorphisms |
| title | The association between matrix metalloprotease-9 gene polymorphisms and primary angle-closure glaucoma in a Chinese Han population |
| title_full | The association between matrix metalloprotease-9 gene polymorphisms and primary angle-closure glaucoma in a Chinese Han population |
| title_fullStr | The association between matrix metalloprotease-9 gene polymorphisms and primary angle-closure glaucoma in a Chinese Han population |
| title_full_unstemmed | The association between matrix metalloprotease-9 gene polymorphisms and primary angle-closure glaucoma in a Chinese Han population |
| title_short | The association between matrix metalloprotease-9 gene polymorphisms and primary angle-closure glaucoma in a Chinese Han population |
| title_sort | association between matrix metalloprotease 9 gene polymorphisms and primary angle closure glaucoma in a chinese han population |
| topic | matrix metalloprotease-9 gene primary angle-closure glaucoma single nucleotide polymorphisms single nucleotide polymorphisms |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067648/ |
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