Effect of High Static Magnetic Fields on Biological Activities and Iron Metabolism in MLO-Y4 Osteocyte-like Cells
There are numerous studies that investigate the effects of static magnetic fields (SMFs) on osteoblasts and osteoclasts. However, although osteocytes are the most abundant cell type in bone tissue, there are few studies on the biological effects of osteocytes under magnetic fields. Iron is a necessa...
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2021-12-01
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| author | Jiancheng Yang Gejing Zhang Qingmei Li Qinghua Tang Yan Feng Peng Shang Yuhong Zeng |
| author_facet | Jiancheng Yang Gejing Zhang Qingmei Li Qinghua Tang Yan Feng Peng Shang Yuhong Zeng |
| author_sort | Jiancheng Yang |
| collection | DOAJ |
| description | There are numerous studies that investigate the effects of static magnetic fields (SMFs) on osteoblasts and osteoclasts. However, although osteocytes are the most abundant cell type in bone tissue, there are few studies on the biological effects of osteocytes under magnetic fields. Iron is a necessary microelement that is involved in numerous life activities in cells. Studies have shown that high static magnetic fields (HiSMF) can regulate cellular iron metabolism. To illustrate the effect of HiSMF on activities of osteocytes, and whether iron is involved in this process, HiSMF of 16 tesla (T) was used, and the changes in cellular morphology, cytoskeleton, function-related protein expression, secretion of various cytokines, and iron metabolism in osteocytes under HiSMF were studied. In addition, the biological effects of HiSMF combined with iron preparation and iron chelator on osteocytes were also investigated. The results showed that HiSMF promoted cellular viability, decreased apoptosis, increased the fractal dimension of the cytoskeleton, altered the secretion of cytokines, and increased iron levels in osteocytes. Moreover, it was found that the biological effects of osteocytes under HiSMF are attenuated or enhanced by treatment with a certain concentration of iron. These data suggest that HiSMF-regulated cellular iron metabolism may be involved in altering the biological effects of osteocytes under HiSMF exposure. |
| first_indexed | 2024-03-10T04:25:55Z |
| format | Article |
| id | doaj.art-911adecdf6eb4bfb8cd8c16573ea0a05 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T04:25:55Z |
| publishDate | 2021-12-01 |
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| spelling | doaj.art-911adecdf6eb4bfb8cd8c16573ea0a052023-11-23T07:38:52ZengMDPI AGCells2073-44092021-12-011012351910.3390/cells10123519Effect of High Static Magnetic Fields on Biological Activities and Iron Metabolism in MLO-Y4 Osteocyte-like CellsJiancheng Yang0Gejing Zhang1Qingmei Li2Qinghua Tang3Yan Feng4Peng Shang5Yuhong Zeng6Department of Osteoporosis, Honghui Hospital, Xi’an Jiaotong University, Xi’an 710054, ChinaKey Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaDepartment of Osteoporosis, Honghui Hospital, Xi’an Jiaotong University, Xi’an 710054, ChinaDepartment of Osteoporosis, Honghui Hospital, Xi’an Jiaotong University, Xi’an 710054, ChinaDepartment of Osteoporosis, Honghui Hospital, Xi’an Jiaotong University, Xi’an 710054, ChinaKey Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaDepartment of Osteoporosis, Honghui Hospital, Xi’an Jiaotong University, Xi’an 710054, ChinaThere are numerous studies that investigate the effects of static magnetic fields (SMFs) on osteoblasts and osteoclasts. However, although osteocytes are the most abundant cell type in bone tissue, there are few studies on the biological effects of osteocytes under magnetic fields. Iron is a necessary microelement that is involved in numerous life activities in cells. Studies have shown that high static magnetic fields (HiSMF) can regulate cellular iron metabolism. To illustrate the effect of HiSMF on activities of osteocytes, and whether iron is involved in this process, HiSMF of 16 tesla (T) was used, and the changes in cellular morphology, cytoskeleton, function-related protein expression, secretion of various cytokines, and iron metabolism in osteocytes under HiSMF were studied. In addition, the biological effects of HiSMF combined with iron preparation and iron chelator on osteocytes were also investigated. The results showed that HiSMF promoted cellular viability, decreased apoptosis, increased the fractal dimension of the cytoskeleton, altered the secretion of cytokines, and increased iron levels in osteocytes. Moreover, it was found that the biological effects of osteocytes under HiSMF are attenuated or enhanced by treatment with a certain concentration of iron. These data suggest that HiSMF-regulated cellular iron metabolism may be involved in altering the biological effects of osteocytes under HiSMF exposure.https://www.mdpi.com/2073-4409/10/12/3519high static magnetic fieldsosteocytesiron metabolismcell viabilitycytoskeletonapoptosis |
| spellingShingle | Jiancheng Yang Gejing Zhang Qingmei Li Qinghua Tang Yan Feng Peng Shang Yuhong Zeng Effect of High Static Magnetic Fields on Biological Activities and Iron Metabolism in MLO-Y4 Osteocyte-like Cells Cells high static magnetic fields osteocytes iron metabolism cell viability cytoskeleton apoptosis |
| title | Effect of High Static Magnetic Fields on Biological Activities and Iron Metabolism in MLO-Y4 Osteocyte-like Cells |
| title_full | Effect of High Static Magnetic Fields on Biological Activities and Iron Metabolism in MLO-Y4 Osteocyte-like Cells |
| title_fullStr | Effect of High Static Magnetic Fields on Biological Activities and Iron Metabolism in MLO-Y4 Osteocyte-like Cells |
| title_full_unstemmed | Effect of High Static Magnetic Fields on Biological Activities and Iron Metabolism in MLO-Y4 Osteocyte-like Cells |
| title_short | Effect of High Static Magnetic Fields on Biological Activities and Iron Metabolism in MLO-Y4 Osteocyte-like Cells |
| title_sort | effect of high static magnetic fields on biological activities and iron metabolism in mlo y4 osteocyte like cells |
| topic | high static magnetic fields osteocytes iron metabolism cell viability cytoskeleton apoptosis |
| url | https://www.mdpi.com/2073-4409/10/12/3519 |
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