Impact of Molecular Weight Variations in <i>Dendrobium officinale</i> Polysaccharides on Antioxidant Activity and Anti-Obesity in <i>Caenorhabditis elegans</i>
This research investigates the impact of <i>Dendrobium officinale</i> polysaccharides (DOP) with different molecular weights on antioxidant effects, lifespan enhancement, and obesity reduction, utilizing both in vitro analyses and the <i>Caenorhabditis elegans</i> (<i>C...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/7/1040 |
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| author | Xiao Pang Heqin Wang Chunbo Guan Qiufeng Chen Xinwen Cui Xiuqing Zhang |
| author_facet | Xiao Pang Heqin Wang Chunbo Guan Qiufeng Chen Xinwen Cui Xiuqing Zhang |
| author_sort | Xiao Pang |
| collection | DOAJ |
| description | This research investigates the impact of <i>Dendrobium officinale</i> polysaccharides (DOP) with different molecular weights on antioxidant effects, lifespan enhancement, and obesity reduction, utilizing both in vitro analyses and the <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) model. Through a series of experiments—ranging from the extraction and modification of polysaccharides, Gel Permeation Chromatography (GPC), and analysis of composition to the evaluation of antioxidant capabilities, this study thoroughly examines DOP and its derivatives (DOP5, DOP15, DOP25) produced via H<sub>2</sub>O<sub>2</sub>-Fe<sup>2+</sup> degradation. The results reveal a direct relationship between the molecular weight of polysaccharides and their bioactivity. Notably, DOP5, with its intermediate molecular weight, demonstrated superior antioxidant properties, significantly extended the lifespan, and improved the health of <i>C. elegans.</i> Furthermore, DOP15 appeared to regulate lipid metabolism by affecting crucial lipid metabolism genes, including fat-4, fat-5, fat-6, sbp-1, and acs-2. These findings highlight the potential application of DOP derivatives as natural antioxidants and agents against obesity, contributing to the development of functional foods and dietary supplements. |
| first_indexed | 2024-04-24T10:44:40Z |
| format | Article |
| id | doaj.art-91223464403640418fbd4957afc948eb |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-24T10:44:40Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-91223464403640418fbd4957afc948eb2024-04-12T13:18:31ZengMDPI AGFoods2304-81582024-03-01137104010.3390/foods13071040Impact of Molecular Weight Variations in <i>Dendrobium officinale</i> Polysaccharides on Antioxidant Activity and Anti-Obesity in <i>Caenorhabditis elegans</i>Xiao Pang0Heqin Wang1Chunbo Guan2Qiufeng Chen3Xinwen Cui4Xiuqing Zhang5College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaThis research investigates the impact of <i>Dendrobium officinale</i> polysaccharides (DOP) with different molecular weights on antioxidant effects, lifespan enhancement, and obesity reduction, utilizing both in vitro analyses and the <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) model. Through a series of experiments—ranging from the extraction and modification of polysaccharides, Gel Permeation Chromatography (GPC), and analysis of composition to the evaluation of antioxidant capabilities, this study thoroughly examines DOP and its derivatives (DOP5, DOP15, DOP25) produced via H<sub>2</sub>O<sub>2</sub>-Fe<sup>2+</sup> degradation. The results reveal a direct relationship between the molecular weight of polysaccharides and their bioactivity. Notably, DOP5, with its intermediate molecular weight, demonstrated superior antioxidant properties, significantly extended the lifespan, and improved the health of <i>C. elegans.</i> Furthermore, DOP15 appeared to regulate lipid metabolism by affecting crucial lipid metabolism genes, including fat-4, fat-5, fat-6, sbp-1, and acs-2. These findings highlight the potential application of DOP derivatives as natural antioxidants and agents against obesity, contributing to the development of functional foods and dietary supplements.https://www.mdpi.com/2304-8158/13/7/1040<i>Dendrobium officinale</i>structural modificationantioxidantobesity<i>C. elegans</i> |
| spellingShingle | Xiao Pang Heqin Wang Chunbo Guan Qiufeng Chen Xinwen Cui Xiuqing Zhang Impact of Molecular Weight Variations in <i>Dendrobium officinale</i> Polysaccharides on Antioxidant Activity and Anti-Obesity in <i>Caenorhabditis elegans</i> Foods <i>Dendrobium officinale</i> structural modification antioxidant obesity <i>C. elegans</i> |
| title | Impact of Molecular Weight Variations in <i>Dendrobium officinale</i> Polysaccharides on Antioxidant Activity and Anti-Obesity in <i>Caenorhabditis elegans</i> |
| title_full | Impact of Molecular Weight Variations in <i>Dendrobium officinale</i> Polysaccharides on Antioxidant Activity and Anti-Obesity in <i>Caenorhabditis elegans</i> |
| title_fullStr | Impact of Molecular Weight Variations in <i>Dendrobium officinale</i> Polysaccharides on Antioxidant Activity and Anti-Obesity in <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | Impact of Molecular Weight Variations in <i>Dendrobium officinale</i> Polysaccharides on Antioxidant Activity and Anti-Obesity in <i>Caenorhabditis elegans</i> |
| title_short | Impact of Molecular Weight Variations in <i>Dendrobium officinale</i> Polysaccharides on Antioxidant Activity and Anti-Obesity in <i>Caenorhabditis elegans</i> |
| title_sort | impact of molecular weight variations in i dendrobium officinale i polysaccharides on antioxidant activity and anti obesity in i caenorhabditis elegans i |
| topic | <i>Dendrobium officinale</i> structural modification antioxidant obesity <i>C. elegans</i> |
| url | https://www.mdpi.com/2304-8158/13/7/1040 |
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