Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine production
Abstract Background l-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, l-ornithine production is based on microbial fermentation, and few microbes are used for producing l-ornithine owing to unsatisfactory production titer. Results In th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-09-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12934-017-0776-8 |
| _version_ | 1811282829962641408 |
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| author | Bin Zhang Miao Yu Ying Zhou Yixue Li Bang-Ce Ye |
| author_facet | Bin Zhang Miao Yu Ying Zhou Yixue Li Bang-Ce Ye |
| author_sort | Bin Zhang |
| collection | DOAJ |
| description | Abstract Background l-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, l-ornithine production is based on microbial fermentation, and few microbes are used for producing l-ornithine owing to unsatisfactory production titer. Results In this study, Corynebacterium glutamicum S9114, a high glutamate-producing strain, was developed for l-ornithine production by pathway engineering. First, argF was deleted to block l-ornithine to citrulline conversion. To improve l-ornithine production, ncgl1221 encoding glutamate transporter, argR encoding arginine repressor, and putP encoding proline transporter were disrupted. This base strain was further engineered by attenuating oxoglutarate dehydrogenase to increase l-ornithine production. Plasmid-based overexpression of argCJBD operon and lysine/arginine transport protein LysE was tested to strengthen l-ornithine synthesis and transportation. This resulted in efficient l-ornithine production at a titer of 18.4 g/L. Conclusion These results demonstrate the potential of Corynebacterium glutamicum S9114 for efficient l-ornithine production and provide new targets for strain development. |
| first_indexed | 2024-04-13T01:59:34Z |
| format | Article |
| id | doaj.art-912517fc735449acae6b0cc672ba80c2 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-13T01:59:34Z |
| publishDate | 2017-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-912517fc735449acae6b0cc672ba80c22022-12-22T03:07:39ZengBMCMicrobial Cell Factories1475-28592017-09-0116111010.1186/s12934-017-0776-8Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine productionBin Zhang0Miao Yu1Ying Zhou2Yixue Li3Bang-Ce Ye4Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyLaboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyLaboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyKey Laboratory of Systems Biology, Shanghai Institute for Biological Sciences, Chinese Academy of SciencesLaboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyAbstract Background l-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, l-ornithine production is based on microbial fermentation, and few microbes are used for producing l-ornithine owing to unsatisfactory production titer. Results In this study, Corynebacterium glutamicum S9114, a high glutamate-producing strain, was developed for l-ornithine production by pathway engineering. First, argF was deleted to block l-ornithine to citrulline conversion. To improve l-ornithine production, ncgl1221 encoding glutamate transporter, argR encoding arginine repressor, and putP encoding proline transporter were disrupted. This base strain was further engineered by attenuating oxoglutarate dehydrogenase to increase l-ornithine production. Plasmid-based overexpression of argCJBD operon and lysine/arginine transport protein LysE was tested to strengthen l-ornithine synthesis and transportation. This resulted in efficient l-ornithine production at a titer of 18.4 g/L. Conclusion These results demonstrate the potential of Corynebacterium glutamicum S9114 for efficient l-ornithine production and provide new targets for strain development.http://link.springer.com/article/10.1186/s12934-017-0776-8Corynebacterium glutamicuml-Ornithine productionMetabolic engineering |
| spellingShingle | Bin Zhang Miao Yu Ying Zhou Yixue Li Bang-Ce Ye Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine production Microbial Cell Factories Corynebacterium glutamicum l-Ornithine production Metabolic engineering |
| title | Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine production |
| title_full | Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine production |
| title_fullStr | Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine production |
| title_full_unstemmed | Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine production |
| title_short | Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine production |
| title_sort | systematic pathway engineering of corynebacterium glutamicum s9114 for l ornithine production |
| topic | Corynebacterium glutamicum l-Ornithine production Metabolic engineering |
| url | http://link.springer.com/article/10.1186/s12934-017-0776-8 |
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