Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet
Omega-3 polyunsaturated fatty acids (n-3 PUFA) can modulate the immune system and their primary effect is on macrophage function. Paracoccidioidomycosis (PCM) is an endemic systemic mycosis in Latin America that is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb). Macrophages are th...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2012-05-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000300003&lng=en&tlng=en |
| _version_ | 1797703193143017472 |
|---|---|
| author | Sheisa Cyléia Sargi Márcia Machado de Oliveira Dalalio Jesuí Vergílio Visentainer Rafael Campos Bezerra João Ângelo de Lima Perini Flávia Braidotti Stevanato Jeane Eliete Laguila Visentainer |
| author_facet | Sheisa Cyléia Sargi Márcia Machado de Oliveira Dalalio Jesuí Vergílio Visentainer Rafael Campos Bezerra João Ângelo de Lima Perini Flávia Braidotti Stevanato Jeane Eliete Laguila Visentainer |
| author_sort | Sheisa Cyléia Sargi |
| collection | DOAJ |
| description | Omega-3 polyunsaturated fatty acids (n-3 PUFA) can modulate the immune system and their primary effect is on macrophage function. Paracoccidioidomycosis (PCM) is an endemic systemic mycosis in Latin America that is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb). Macrophages are the main defence against this pathogen and have microbicidal activity that is dependent on interferon-Γ and tumour necrosis factor (TNF)-α. These cytokines stimulate the synthesis of nitric oxide (NO) and hydrogen peroxide (H2O2), leading to the death of the fungus. To study the effect of n-3 PUFA on the host immune response during experimental PCM, macrophages that were obtained from animals infected with Pb18 and fed a diet enriched by linseed (LIN) oil were cultured and challenged with the fungus in vitro. The macrophage function was analysed based on the concentrations of TNF-α, NO and H2O2. LIN oil seems to influence the production of TNF-α during the development of disease. A diet enriched with LIN oil influences the microbicidal activity of the macrophages by inducing the production of cytokines and metabolites such as NO and H2O2, predominantly in the chronic phase of infection. |
| first_indexed | 2024-03-12T05:01:02Z |
| format | Article |
| id | doaj.art-912524a629934b55ab0cd5afc9493cb4 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:01:02Z |
| publishDate | 2012-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-912524a629934b55ab0cd5afc9493cb42023-09-03T09:11:44ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602012-05-01107330330910.1590/S0074-02762012000300003S0074-02762012000300003Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched dietSheisa Cyléia Sargi0Márcia Machado de Oliveira Dalalio1Jesuí Vergílio Visentainer2Rafael Campos Bezerra3João Ângelo de Lima Perini4Flávia Braidotti StevanatoJeane Eliete Laguila Visentainer5Universidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáOmega-3 polyunsaturated fatty acids (n-3 PUFA) can modulate the immune system and their primary effect is on macrophage function. Paracoccidioidomycosis (PCM) is an endemic systemic mycosis in Latin America that is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb). Macrophages are the main defence against this pathogen and have microbicidal activity that is dependent on interferon-Γ and tumour necrosis factor (TNF)-α. These cytokines stimulate the synthesis of nitric oxide (NO) and hydrogen peroxide (H2O2), leading to the death of the fungus. To study the effect of n-3 PUFA on the host immune response during experimental PCM, macrophages that were obtained from animals infected with Pb18 and fed a diet enriched by linseed (LIN) oil were cultured and challenged with the fungus in vitro. The macrophage function was analysed based on the concentrations of TNF-α, NO and H2O2. LIN oil seems to influence the production of TNF-α during the development of disease. A diet enriched with LIN oil influences the microbicidal activity of the macrophages by inducing the production of cytokines and metabolites such as NO and H2O2, predominantly in the chronic phase of infection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000300003&lng=en&tlng=enmacrophagesParacoccidioides brasiliensiscytokinesn-3 polyunsaturated fatty acids |
| spellingShingle | Sheisa Cyléia Sargi Márcia Machado de Oliveira Dalalio Jesuí Vergílio Visentainer Rafael Campos Bezerra João Ângelo de Lima Perini Flávia Braidotti Stevanato Jeane Eliete Laguila Visentainer Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet Memorias do Instituto Oswaldo Cruz macrophages Paracoccidioides brasiliensis cytokines n-3 polyunsaturated fatty acids |
| title | Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet |
| title_full | Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet |
| title_fullStr | Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet |
| title_full_unstemmed | Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet |
| title_short | Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet |
| title_sort | production of tnf α nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil enriched diet |
| topic | macrophages Paracoccidioides brasiliensis cytokines n-3 polyunsaturated fatty acids |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000300003&lng=en&tlng=en |
| work_keys_str_mv | AT sheisacyleiasargi productionoftnfanitricoxideandhydrogenperoxidebymacrophagesfrommicewithparacoccidioidomycosisthatwerefedalinseedoilenricheddiet AT marciamachadodeoliveiradalalio productionoftnfanitricoxideandhydrogenperoxidebymacrophagesfrommicewithparacoccidioidomycosisthatwerefedalinseedoilenricheddiet AT jesuivergiliovisentainer productionoftnfanitricoxideandhydrogenperoxidebymacrophagesfrommicewithparacoccidioidomycosisthatwerefedalinseedoilenricheddiet AT rafaelcamposbezerra productionoftnfanitricoxideandhydrogenperoxidebymacrophagesfrommicewithparacoccidioidomycosisthatwerefedalinseedoilenricheddiet AT joaoangelodelimaperini productionoftnfanitricoxideandhydrogenperoxidebymacrophagesfrommicewithparacoccidioidomycosisthatwerefedalinseedoilenricheddiet AT flaviabraidottistevanato productionoftnfanitricoxideandhydrogenperoxidebymacrophagesfrommicewithparacoccidioidomycosisthatwerefedalinseedoilenricheddiet AT jeaneelietelaguilavisentainer productionoftnfanitricoxideandhydrogenperoxidebymacrophagesfrommicewithparacoccidioidomycosisthatwerefedalinseedoilenricheddiet |