Diagnosis of Toxoplasma Infection in Allogenic Pre HCTSP Patients Using Molecular Methods
Background: We aimed to estimate the incidence of Toxoplasma infection in T. gondii-seropositive patients under allogeneic hematopoietic stem cell transplantation (HSCT). Methods: The present research was a prospective study on 54 whole blood samples of allogeneic HSCT recipients, who were refer...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2022-05-01
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| Series: | Iranian Journal of Parasitology |
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| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3336 |
| _version_ | 1818235338834837504 |
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| author | Meysam Yusefi Zahra Arab-Mazar Shirzad Fallahi Amirreza Javadi Mamaghani Shahnaz Sali Naeem Nikpour Meisam Barati Arian Karimi Rouzbahani Farnaz Kheirandish |
| author_facet | Meysam Yusefi Zahra Arab-Mazar Shirzad Fallahi Amirreza Javadi Mamaghani Shahnaz Sali Naeem Nikpour Meisam Barati Arian Karimi Rouzbahani Farnaz Kheirandish |
| author_sort | Meysam Yusefi |
| collection | DOAJ |
| description |
Background: We aimed to estimate the incidence of Toxoplasma infection in T. gondii-seropositive patients under allogeneic hematopoietic stem cell transplantation (HSCT).
Methods: The present research was a prospective study on 54 whole blood samples of allogeneic HSCT recipients, who were referring to bone narrow transplantation centers affiliated to Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2018. All patients were IgG positive against T. gondii.
Results: Overall, 54 Toxoplasma positive pre-HCTSP patients were enrolled. 53.7% (n= 29) were male, also 1.9% (n=1) had germ-line type of the disease. The Multiple myeloma patients had higher age in comparison with other disease, but pairwise comparison showed the difference of age between Multiple myeloma patients were statistically significant with Acute lymphoblastic leukemia, Acute myeloblastic leukemia and Huntington's disease (P< 0.05). The results of PCR assay showed 5.6% (n= 3) of the patients were infected with Toxoplasma.
Conclusion: PCR method has detected considerable incidence of Toxoplasma infection for monitoring HSCT recipients at risk for toxoplasmosis, and many patients who showed the incidence of toxoplasmosis had previous infections with the Toxoplasma parasite.
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| first_indexed | 2024-12-12T11:52:23Z |
| format | Article |
| id | doaj.art-912797e45d974c87bfd96730705f113e |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-12T11:52:23Z |
| publishDate | 2022-05-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-912797e45d974c87bfd96730705f113e2022-12-22T00:25:16ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2022-05-0117210.18502/ijpa.v17i2.9541Diagnosis of Toxoplasma Infection in Allogenic Pre HCTSP Patients Using Molecular MethodsMeysam Yusefi0Zahra Arab-Mazar1Shirzad Fallahi2Amirreza Javadi Mamaghani3Shahnaz Sali4Naeem Nikpour5Meisam Barati6Arian Karimi Rouzbahani7Farnaz Kheirandish81. Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2. Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran1. Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranHepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran AND Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, IranDepartment of Parasitology and Mycology, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, IranInfectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Hematology and Oncology, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Cellular and Molecular Nutrition, Shahid Beheshti University of Medical Sciences, Tehran, Iran1. Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran 2. USERN Office, Lorestan University of Medical Sciences, Khorramabad, IranDepartment of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran 2. Razi Herbal Medicines Research Center, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran Background: We aimed to estimate the incidence of Toxoplasma infection in T. gondii-seropositive patients under allogeneic hematopoietic stem cell transplantation (HSCT). Methods: The present research was a prospective study on 54 whole blood samples of allogeneic HSCT recipients, who were referring to bone narrow transplantation centers affiliated to Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2018. All patients were IgG positive against T. gondii. Results: Overall, 54 Toxoplasma positive pre-HCTSP patients were enrolled. 53.7% (n= 29) were male, also 1.9% (n=1) had germ-line type of the disease. The Multiple myeloma patients had higher age in comparison with other disease, but pairwise comparison showed the difference of age between Multiple myeloma patients were statistically significant with Acute lymphoblastic leukemia, Acute myeloblastic leukemia and Huntington's disease (P< 0.05). The results of PCR assay showed 5.6% (n= 3) of the patients were infected with Toxoplasma. Conclusion: PCR method has detected considerable incidence of Toxoplasma infection for monitoring HSCT recipients at risk for toxoplasmosis, and many patients who showed the incidence of toxoplasmosis had previous infections with the Toxoplasma parasite. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3336ToxoplasmaHematopoietic stem cell transplantationToxoplasmosis |
| spellingShingle | Meysam Yusefi Zahra Arab-Mazar Shirzad Fallahi Amirreza Javadi Mamaghani Shahnaz Sali Naeem Nikpour Meisam Barati Arian Karimi Rouzbahani Farnaz Kheirandish Diagnosis of Toxoplasma Infection in Allogenic Pre HCTSP Patients Using Molecular Methods Iranian Journal of Parasitology Toxoplasma Hematopoietic stem cell transplantation Toxoplasmosis |
| title | Diagnosis of Toxoplasma Infection in Allogenic Pre HCTSP Patients Using Molecular Methods |
| title_full | Diagnosis of Toxoplasma Infection in Allogenic Pre HCTSP Patients Using Molecular Methods |
| title_fullStr | Diagnosis of Toxoplasma Infection in Allogenic Pre HCTSP Patients Using Molecular Methods |
| title_full_unstemmed | Diagnosis of Toxoplasma Infection in Allogenic Pre HCTSP Patients Using Molecular Methods |
| title_short | Diagnosis of Toxoplasma Infection in Allogenic Pre HCTSP Patients Using Molecular Methods |
| title_sort | diagnosis of toxoplasma infection in allogenic pre hctsp patients using molecular methods |
| topic | Toxoplasma Hematopoietic stem cell transplantation Toxoplasmosis |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3336 |
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