Isolation and immunoblotting of somatic antigens of Cotylophoron cotylophorum and Gastrothylax crumenifer
Paramphistomosis is the emerging fluke infection in young ruminants which results in high morbidity and mortality in tropics and sub-tropics. The present study was carried out to detect the sharing antigens among the purified somatic antigens of G. crumenifer and C. cotylophorum, in order to use a...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2020-02-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/98173 |
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| author | R VIJAYASHANTHI C SOUNDARARAJAN M RAMAN T M A SENTHILKUMAR |
| author_facet | R VIJAYASHANTHI C SOUNDARARAJAN M RAMAN T M A SENTHILKUMAR |
| author_sort | R VIJAYASHANTHI |
| collection | DOAJ |
| description |
Paramphistomosis is the emerging fluke infection in young ruminants which results in high morbidity and mortality in tropics and sub-tropics. The present study was carried out to detect the sharing antigens among the purified somatic antigens of G. crumenifer and C. cotylophorum, in order to use as a candidate antigen for diagnosis of paramphistomosis in sheep. Immunoblot analysis of the purified somatic antigens of C. cotylophorum and G. crumenifer using their specific rabbit hyper immune serum revealed 3 common polypeptides at the range of 27, 29 and 60 kDa. Hence these three immunodominant peptides can be used candidate antigens in development of vaccines against ovine paraphistomosis and also for early diagnosis of infection.
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| first_indexed | 2024-04-10T07:47:48Z |
| format | Article |
| id | doaj.art-91291d3dfd864adb826ea49c1708c615 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:47:48Z |
| publishDate | 2020-02-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-91291d3dfd864adb826ea49c1708c6152023-02-23T10:28:04ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272020-02-0190110.56093/ijans.v90i1.98173Isolation and immunoblotting of somatic antigens of Cotylophoron cotylophorum and Gastrothylax crumeniferR VIJAYASHANTHI0C SOUNDARARAJAN1M RAMAN2T M A SENTHILKUMAR3Junior Research Fellow, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 IndiaProfessor, Department of Veterinary Parasitology, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 IndiaProfessor and Project Director, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 IndiaProfessor, Department of Animal Biotechnology, Madras Veterinary College, Chennai Paramphistomosis is the emerging fluke infection in young ruminants which results in high morbidity and mortality in tropics and sub-tropics. The present study was carried out to detect the sharing antigens among the purified somatic antigens of G. crumenifer and C. cotylophorum, in order to use as a candidate antigen for diagnosis of paramphistomosis in sheep. Immunoblot analysis of the purified somatic antigens of C. cotylophorum and G. crumenifer using their specific rabbit hyper immune serum revealed 3 common polypeptides at the range of 27, 29 and 60 kDa. Hence these three immunodominant peptides can be used candidate antigens in development of vaccines against ovine paraphistomosis and also for early diagnosis of infection. https://epubs.icar.org.in/index.php/IJAnS/article/view/98173ParamphistomesSomatic antigenSDS-PAGEWestern blot |
| spellingShingle | R VIJAYASHANTHI C SOUNDARARAJAN M RAMAN T M A SENTHILKUMAR Isolation and immunoblotting of somatic antigens of Cotylophoron cotylophorum and Gastrothylax crumenifer Indian Journal of Animal Sciences Paramphistomes Somatic antigen SDS-PAGE Western blot |
| title | Isolation and immunoblotting of somatic antigens of Cotylophoron cotylophorum and Gastrothylax crumenifer |
| title_full | Isolation and immunoblotting of somatic antigens of Cotylophoron cotylophorum and Gastrothylax crumenifer |
| title_fullStr | Isolation and immunoblotting of somatic antigens of Cotylophoron cotylophorum and Gastrothylax crumenifer |
| title_full_unstemmed | Isolation and immunoblotting of somatic antigens of Cotylophoron cotylophorum and Gastrothylax crumenifer |
| title_short | Isolation and immunoblotting of somatic antigens of Cotylophoron cotylophorum and Gastrothylax crumenifer |
| title_sort | isolation and immunoblotting of somatic antigens of cotylophoron cotylophorum and gastrothylax crumenifer |
| topic | Paramphistomes Somatic antigen SDS-PAGE Western blot |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/98173 |
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