An improved formula for standard hypoxia tolerance time (STT) to evaluate hypoxic tolerance in mice
Abstract Background Hypoxia is a primary cause of mountain sickness and a common pathological condition in patients with heart failure, shock, stroke, and chronic obstructive pulmonary disease (COPD). Thus far, little advancement in countering hypoxic damage has been achieved, and one of the main re...
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | Military Medical Research |
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| Online Access: | http://link.springer.com/article/10.1186/s40779-018-0180-7 |
| _version_ | 1818218898579783680 |
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| author | Gang Xu Yu-Qi Gao Yi-Xing Gao Gang Wu Jian-Yang Zhang Wen-Xiang Gao |
| author_facet | Gang Xu Yu-Qi Gao Yi-Xing Gao Gang Wu Jian-Yang Zhang Wen-Xiang Gao |
| author_sort | Gang Xu |
| collection | DOAJ |
| description | Abstract Background Hypoxia is a primary cause of mountain sickness and a common pathological condition in patients with heart failure, shock, stroke, and chronic obstructive pulmonary disease (COPD). Thus far, little advancement in countering hypoxic damage has been achieved, and one of the main reasons is the absence of an ideal algorithm or calculation method to normalize hypoxia tolerance scores when evaluating an animal model. In this study, we improved a traditional calculation formula for assessment of hypoxia tolerance. Methods We used a sealed bottle model in which the oxygen is gradually consumed by a mouse inside. To evaluate the hypoxia tolerance of mice, the survival time (ST) of the mouse is recorded and was used to calculate standard hypoxia tolerance time (STT) and adjusted standard hypoxia tolerance time (ASTT). Mice administered with methazolamide and saline were used as positive and negative controls, respectively. Results Since mice were grouped according to either body weight (BW) or bottle volume, we found a strongly negative correlation between STT and BW instead of between STT and bottle volume, suggesting that different BWs could cause false positive or negative errors in the STT results. Furthermore, both false positive and negative errors could be rectified when ASTT was used as the evaluation index. Screening for anti-hypoxic medicines by using mice as the experimental subjects would provide more credible results with the improved ASTT method than with the STT method. Conclusion ASTT could be a better index than STT for the evaluation of hypoxia tolerance abilities as it could eliminate the impact of animal BW. |
| first_indexed | 2024-12-12T07:31:05Z |
| format | Article |
| id | doaj.art-91292cec868a41719dfe80d30e53d5f7 |
| institution | Directory Open Access Journal |
| issn | 2054-9369 |
| language | English |
| last_indexed | 2024-12-12T07:31:05Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Military Medical Research |
| spelling | doaj.art-91292cec868a41719dfe80d30e53d5f72022-12-22T00:33:01ZengBMCMilitary Medical Research2054-93692018-09-01511610.1186/s40779-018-0180-7An improved formula for standard hypoxia tolerance time (STT) to evaluate hypoxic tolerance in miceGang Xu0Yu-Qi Gao1Yi-Xing Gao2Gang Wu3Jian-Yang Zhang4Wen-Xiang Gao5Institute of Medicine and Hygienic Equipment for High Altitude Region, College of High Altitude Military Medicine, Army Medical University (Third Military Medical University)Institute of Medicine and Hygienic Equipment for High Altitude Region, College of High Altitude Military Medicine, Army Medical University (Third Military Medical University)Key Laboratory of High Altitude Medicine, People’s Liberation ArmyInstitute of Medicine and Hygienic Equipment for High Altitude Region, College of High Altitude Military Medicine, Army Medical University (Third Military Medical University)Institute of Medicine and Hygienic Equipment for High Altitude Region, College of High Altitude Military Medicine, Army Medical University (Third Military Medical University)Institute of Medicine and Hygienic Equipment for High Altitude Region, College of High Altitude Military Medicine, Army Medical University (Third Military Medical University)Abstract Background Hypoxia is a primary cause of mountain sickness and a common pathological condition in patients with heart failure, shock, stroke, and chronic obstructive pulmonary disease (COPD). Thus far, little advancement in countering hypoxic damage has been achieved, and one of the main reasons is the absence of an ideal algorithm or calculation method to normalize hypoxia tolerance scores when evaluating an animal model. In this study, we improved a traditional calculation formula for assessment of hypoxia tolerance. Methods We used a sealed bottle model in which the oxygen is gradually consumed by a mouse inside. To evaluate the hypoxia tolerance of mice, the survival time (ST) of the mouse is recorded and was used to calculate standard hypoxia tolerance time (STT) and adjusted standard hypoxia tolerance time (ASTT). Mice administered with methazolamide and saline were used as positive and negative controls, respectively. Results Since mice were grouped according to either body weight (BW) or bottle volume, we found a strongly negative correlation between STT and BW instead of between STT and bottle volume, suggesting that different BWs could cause false positive or negative errors in the STT results. Furthermore, both false positive and negative errors could be rectified when ASTT was used as the evaluation index. Screening for anti-hypoxic medicines by using mice as the experimental subjects would provide more credible results with the improved ASTT method than with the STT method. Conclusion ASTT could be a better index than STT for the evaluation of hypoxia tolerance abilities as it could eliminate the impact of animal BW.http://link.springer.com/article/10.1186/s40779-018-0180-7HypoxiaStandard hypoxic tolerance timeMice modelBody weight |
| spellingShingle | Gang Xu Yu-Qi Gao Yi-Xing Gao Gang Wu Jian-Yang Zhang Wen-Xiang Gao An improved formula for standard hypoxia tolerance time (STT) to evaluate hypoxic tolerance in mice Military Medical Research Hypoxia Standard hypoxic tolerance time Mice model Body weight |
| title | An improved formula for standard hypoxia tolerance time (STT) to evaluate hypoxic tolerance in mice |
| title_full | An improved formula for standard hypoxia tolerance time (STT) to evaluate hypoxic tolerance in mice |
| title_fullStr | An improved formula for standard hypoxia tolerance time (STT) to evaluate hypoxic tolerance in mice |
| title_full_unstemmed | An improved formula for standard hypoxia tolerance time (STT) to evaluate hypoxic tolerance in mice |
| title_short | An improved formula for standard hypoxia tolerance time (STT) to evaluate hypoxic tolerance in mice |
| title_sort | improved formula for standard hypoxia tolerance time stt to evaluate hypoxic tolerance in mice |
| topic | Hypoxia Standard hypoxic tolerance time Mice model Body weight |
| url | http://link.springer.com/article/10.1186/s40779-018-0180-7 |
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