Estrogen Receptor Alpha Gene (ESR1) Facilitates Th2-immune Response and Enhances Th2 Cytokines in Experimental Atopic Dermatitis Mice
Background: Molecular markers are involved in atopic dermatitis (AD) pathogenesis. The estrogen receptor (ESR)-1 gene, encoding ERα, is reported to express aberrantly in AD patients.Objective: To detect the biological functions of ESR1 in 2,4 dinitrochlorobenzene (DNCB)-treated mice.Methods: The DNC...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2023-06-01
|
| Series: | Iranian Journal of Immunology |
| Subjects: | |
| Online Access: | https://iji.sums.ac.ir/article_49308_75a0c1dbc99a8103f3930b8053d19f1c.pdf |
| _version_ | 1797816266217488384 |
|---|---|
| author | Jianrong Niu Hui Zhou Rong Tian Xudong Wang |
| author_facet | Jianrong Niu Hui Zhou Rong Tian Xudong Wang |
| author_sort | Jianrong Niu |
| collection | DOAJ |
| description | Background: Molecular markers are involved in atopic dermatitis (AD) pathogenesis. The estrogen receptor (ESR)-1 gene, encoding ERα, is reported to express aberrantly in AD patients.Objective: To detect the biological functions of ESR1 in 2,4 dinitrochlorobenzene (DNCB)-treated mice.Methods: The DNCB-treated mice received a topical application of emulsion containing the 1,3-bis(4 hydroxyphenyl)-4-methyl-5-[4-(2-piperidinyl ethoxy) phenol]-1H-pyrazole dihydrochloride (MPP; an ESR1-selective antagonist) to dorsal skins and ears. Then the dermatitis scores, histopathological changes, and cytokine levels were evaluated.Results: MPP specifically downregulated ESR1 expression in DNCB-applied mice. Functionally, application of MPP abolished the DNCB-induced promotion in dermatitis score. Additionally, MPP administration protected against DNCB-induced dermatitis severity, suppressed mast cell infiltration and reduced production of immunoglobulin E (IgE) and thymus and activation-regulated chemokine (TARC). Moreover, MPP treatment inhibited DNCB- induced production of Th2 cytokines and infiltration of CD4+ T cells.Conclusion: ESR1 facilitates Th2-immune response and enhances Th2 cytokines in AD mice. |
| first_indexed | 2024-03-13T08:36:13Z |
| format | Article |
| id | doaj.art-912a4cdf05104a6887b93f65b591b3dd |
| institution | Directory Open Access Journal |
| issn | 1735-1383 1735-367X |
| language | English |
| last_indexed | 2024-03-13T08:36:13Z |
| publishDate | 2023-06-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Immunology |
| spelling | doaj.art-912a4cdf05104a6887b93f65b591b3dd2023-05-30T23:22:06ZengShiraz University of Medical SciencesIranian Journal of Immunology1735-13831735-367X2023-06-0120216717610.22034/iji.2023.97283.249449308Estrogen Receptor Alpha Gene (ESR1) Facilitates Th2-immune Response and Enhances Th2 Cytokines in Experimental Atopic Dermatitis MiceJianrong Niu0Hui Zhou1Rong Tian2Xudong Wang3Department of Dermatology, Air Force Medical Center PLA, Beijing 100142, China.Department of Dermatology, Air Force Medical Center PLA, Beijing 100142, China.Department of Dermatology, Air Force Medical Center PLA, Beijing 100142, China.Southern Medical District of Chinese PLA General Hospital, Beijing 100853, China.Background: Molecular markers are involved in atopic dermatitis (AD) pathogenesis. The estrogen receptor (ESR)-1 gene, encoding ERα, is reported to express aberrantly in AD patients.Objective: To detect the biological functions of ESR1 in 2,4 dinitrochlorobenzene (DNCB)-treated mice.Methods: The DNCB-treated mice received a topical application of emulsion containing the 1,3-bis(4 hydroxyphenyl)-4-methyl-5-[4-(2-piperidinyl ethoxy) phenol]-1H-pyrazole dihydrochloride (MPP; an ESR1-selective antagonist) to dorsal skins and ears. Then the dermatitis scores, histopathological changes, and cytokine levels were evaluated.Results: MPP specifically downregulated ESR1 expression in DNCB-applied mice. Functionally, application of MPP abolished the DNCB-induced promotion in dermatitis score. Additionally, MPP administration protected against DNCB-induced dermatitis severity, suppressed mast cell infiltration and reduced production of immunoglobulin E (IgE) and thymus and activation-regulated chemokine (TARC). Moreover, MPP treatment inhibited DNCB- induced production of Th2 cytokines and infiltration of CD4+ T cells.Conclusion: ESR1 facilitates Th2-immune response and enhances Th2 cytokines in AD mice.https://iji.sums.ac.ir/article_49308_75a0c1dbc99a8103f3930b8053d19f1c.pdfantagonistatopic dermatitisesr1inflammatory cytokinesmice |
| spellingShingle | Jianrong Niu Hui Zhou Rong Tian Xudong Wang Estrogen Receptor Alpha Gene (ESR1) Facilitates Th2-immune Response and Enhances Th2 Cytokines in Experimental Atopic Dermatitis Mice Iranian Journal of Immunology antagonist atopic dermatitis esr1 inflammatory cytokines mice |
| title | Estrogen Receptor Alpha Gene (ESR1) Facilitates Th2-immune Response and Enhances Th2 Cytokines in Experimental Atopic Dermatitis Mice |
| title_full | Estrogen Receptor Alpha Gene (ESR1) Facilitates Th2-immune Response and Enhances Th2 Cytokines in Experimental Atopic Dermatitis Mice |
| title_fullStr | Estrogen Receptor Alpha Gene (ESR1) Facilitates Th2-immune Response and Enhances Th2 Cytokines in Experimental Atopic Dermatitis Mice |
| title_full_unstemmed | Estrogen Receptor Alpha Gene (ESR1) Facilitates Th2-immune Response and Enhances Th2 Cytokines in Experimental Atopic Dermatitis Mice |
| title_short | Estrogen Receptor Alpha Gene (ESR1) Facilitates Th2-immune Response and Enhances Th2 Cytokines in Experimental Atopic Dermatitis Mice |
| title_sort | estrogen receptor alpha gene esr1 facilitates th2 immune response and enhances th2 cytokines in experimental atopic dermatitis mice |
| topic | antagonist atopic dermatitis esr1 inflammatory cytokines mice |
| url | https://iji.sums.ac.ir/article_49308_75a0c1dbc99a8103f3930b8053d19f1c.pdf |
| work_keys_str_mv | AT jianrongniu estrogenreceptoralphageneesr1facilitatesth2immuneresponseandenhancesth2cytokinesinexperimentalatopicdermatitismice AT huizhou estrogenreceptoralphageneesr1facilitatesth2immuneresponseandenhancesth2cytokinesinexperimentalatopicdermatitismice AT rongtian estrogenreceptoralphageneesr1facilitatesth2immuneresponseandenhancesth2cytokinesinexperimentalatopicdermatitismice AT xudongwang estrogenreceptoralphageneesr1facilitatesth2immuneresponseandenhancesth2cytokinesinexperimentalatopicdermatitismice |