EVALUATION OF SIX CAMEL BREEDS FOR HETEROZYGOCITY THROUGH RESTRICTION FRAGMENT LENGTH POLYMORPHISM
In the camel tyrosinase gene, a restriction site provoked by the T variant was used in a special restriction fragment length polymorphism analysis (PCR-RFLP) for genotyping of animals from six different Pakistani camel breeds (Marecha, Dhatti, Larri, Kohi, Campbelpuri and Sakrai). For this purpose,...
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| Format: | Article |
| Language: | English |
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University of Agriculture, Faisalabad
2008-01-01
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| Series: | Pakistan Veterinary Journal |
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| Online Access: | http://pvj.com.pk/pdf-files/28_1/13-16.pdf |
| _version_ | 1811260741842370560 |
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| author | M. G. SHAH, M. REISSMANN1, A. S. Qureshi2 AND H. J. SCHWARTZ3 |
| author_facet | M. G. SHAH, M. REISSMANN1, A. S. Qureshi2 AND H. J. SCHWARTZ3 |
| author_sort | M. G. SHAH, M. REISSMANN1, A. S. Qureshi2 AND H. J. SCHWARTZ3 |
| collection | DOAJ |
| description | In the camel tyrosinase gene, a restriction site provoked by the T variant was used in a special restriction fragment length polymorphism analysis (PCR-RFLP) for genotyping of animals from six different Pakistani camel breeds (Marecha, Dhatti, Larri, Kohi, Campbelpuri and Sakrai). For this purpose, four new primer pairs were designed for sequencing the coding region of exon 1 of the tyrosinase gene. PCR reactions were carried out in a total volume of 25 l using 100 ng genomic DNA to amplify a 474 bp fragment at 56°C. A SNP (T/C) at 200 bps was found and exploited with a Dde I restriction enzyme that resulted in three different genotypes i.e. TT, TC and CC in each studied camel breed. Significant differences in the genotype frequency between the breeds were recorded. The Sakrai breed showed a distinctly higher frequency of heterozygous animals compared to Marecha, Dhatti, Larri and Kohi breeds. Our new designed primers could be used for genotype screening of other camel breeds. However, for understanding the contribution of tyrosinase gene and its antagonist i.e. agouti in the coat colour production, complete sequence of the gene is imperative. |
| first_indexed | 2024-04-12T18:52:07Z |
| format | Article |
| id | doaj.art-912c3baa3b32489195ad7ce9ba8a7906 |
| institution | Directory Open Access Journal |
| issn | 0253-8318 |
| language | English |
| last_indexed | 2024-04-12T18:52:07Z |
| publishDate | 2008-01-01 |
| publisher | University of Agriculture, Faisalabad |
| record_format | Article |
| series | Pakistan Veterinary Journal |
| spelling | doaj.art-912c3baa3b32489195ad7ce9ba8a79062022-12-22T03:20:26ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182008-01-012811316EVALUATION OF SIX CAMEL BREEDS FOR HETEROZYGOCITY THROUGH RESTRICTION FRAGMENT LENGTH POLYMORPHISMM. G. SHAH, M. REISSMANN1, A. S. Qureshi2 AND H. J. SCHWARTZ3In the camel tyrosinase gene, a restriction site provoked by the T variant was used in a special restriction fragment length polymorphism analysis (PCR-RFLP) for genotyping of animals from six different Pakistani camel breeds (Marecha, Dhatti, Larri, Kohi, Campbelpuri and Sakrai). For this purpose, four new primer pairs were designed for sequencing the coding region of exon 1 of the tyrosinase gene. PCR reactions were carried out in a total volume of 25 l using 100 ng genomic DNA to amplify a 474 bp fragment at 56°C. A SNP (T/C) at 200 bps was found and exploited with a Dde I restriction enzyme that resulted in three different genotypes i.e. TT, TC and CC in each studied camel breed. Significant differences in the genotype frequency between the breeds were recorded. The Sakrai breed showed a distinctly higher frequency of heterozygous animals compared to Marecha, Dhatti, Larri and Kohi breeds. Our new designed primers could be used for genotype screening of other camel breeds. However, for understanding the contribution of tyrosinase gene and its antagonist i.e. agouti in the coat colour production, complete sequence of the gene is imperative.http://pvj.com.pk/pdf-files/28_1/13-16.pdfCamelgenotypingRFLPtyrosinase |
| spellingShingle | M. G. SHAH, M. REISSMANN1, A. S. Qureshi2 AND H. J. SCHWARTZ3 EVALUATION OF SIX CAMEL BREEDS FOR HETEROZYGOCITY THROUGH RESTRICTION FRAGMENT LENGTH POLYMORPHISM Pakistan Veterinary Journal Camel genotyping RFLP tyrosinase |
| title | EVALUATION OF SIX CAMEL BREEDS FOR HETEROZYGOCITY THROUGH RESTRICTION FRAGMENT LENGTH POLYMORPHISM |
| title_full | EVALUATION OF SIX CAMEL BREEDS FOR HETEROZYGOCITY THROUGH RESTRICTION FRAGMENT LENGTH POLYMORPHISM |
| title_fullStr | EVALUATION OF SIX CAMEL BREEDS FOR HETEROZYGOCITY THROUGH RESTRICTION FRAGMENT LENGTH POLYMORPHISM |
| title_full_unstemmed | EVALUATION OF SIX CAMEL BREEDS FOR HETEROZYGOCITY THROUGH RESTRICTION FRAGMENT LENGTH POLYMORPHISM |
| title_short | EVALUATION OF SIX CAMEL BREEDS FOR HETEROZYGOCITY THROUGH RESTRICTION FRAGMENT LENGTH POLYMORPHISM |
| title_sort | evaluation of six camel breeds for heterozygocity through restriction fragment length polymorphism |
| topic | Camel genotyping RFLP tyrosinase |
| url | http://pvj.com.pk/pdf-files/28_1/13-16.pdf |
| work_keys_str_mv | AT mgshahmreissmann1asqureshi2andhjschwartz3 evaluationofsixcamelbreedsforheterozygocitythroughrestrictionfragmentlengthpolymorphism |