BMP-2 promotes oral squamous carcinoma cell invasion by inducing CCL5 release.
Bone morphogenetic protein-2 (BMP-2)-containing bone grafts are useful regenerative materials for oral and maxillofacial surgery; however, several in vitro and in vivo studies previously reported cancer progression-related adverse effects caused by BMP-2. In this study, by quantifying the rhBMP-2 co...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4182698?pdf=render |
| _version_ | 1818806900451442688 |
|---|---|
| author | Mi-joo Kim Kwang-mahn Kim Jin Kim Kyoung-nam Kim |
| author_facet | Mi-joo Kim Kwang-mahn Kim Jin Kim Kyoung-nam Kim |
| author_sort | Mi-joo Kim |
| collection | DOAJ |
| description | Bone morphogenetic protein-2 (BMP-2)-containing bone grafts are useful regenerative materials for oral and maxillofacial surgery; however, several in vitro and in vivo studies previously reported cancer progression-related adverse effects caused by BMP-2. In this study, by quantifying the rhBMP-2 content released from bone grafts, the rhBMP-2 concentration that did not show cytotoxicity in each cell line was determined and applied to the in vitro monoculture or coculture model in the invasion assay. Our results showed that 1 ng/ml rhBMP-2, while not affecting cancer cell viability, significantly increased the invasion ability of the cancer cells cocultured with fibroblasts. Cocultured medium with rhBMP-2 also contained increased levels of matrix metalloproteinases. rhBMP-2-treated cocultured fibroblasts did not show a prominent difference in mRNA expression profile. Some cytokines, however, were detected in the conditioned medium by a human cytokine antibody array. Among them, the cancer invasion-related factor CCL5 was quantified by ELISA. Interestingly, CCL5 neutralizing antibodies significantly reduced the invasion of oral cancer cells. In conclusion, our results suggest that 1 ng/ml rhBMP-2 may induce invasion of oral squamous cell carcinoma (OSCC) cells by CCL5 release in coculture models. Therefore, we propose that a careful clinical examination before the use of rhBMP-2-containing biomaterials is indispensable for using rhBMP-2 treatment to prevent cancer progression. |
| first_indexed | 2024-12-18T19:17:07Z |
| format | Article |
| id | doaj.art-91300fbf252843dc98f9048c107fb339 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-18T19:17:07Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-91300fbf252843dc98f9048c107fb3392022-12-21T20:56:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e10817010.1371/journal.pone.0108170BMP-2 promotes oral squamous carcinoma cell invasion by inducing CCL5 release.Mi-joo KimKwang-mahn KimJin KimKyoung-nam KimBone morphogenetic protein-2 (BMP-2)-containing bone grafts are useful regenerative materials for oral and maxillofacial surgery; however, several in vitro and in vivo studies previously reported cancer progression-related adverse effects caused by BMP-2. In this study, by quantifying the rhBMP-2 content released from bone grafts, the rhBMP-2 concentration that did not show cytotoxicity in each cell line was determined and applied to the in vitro monoculture or coculture model in the invasion assay. Our results showed that 1 ng/ml rhBMP-2, while not affecting cancer cell viability, significantly increased the invasion ability of the cancer cells cocultured with fibroblasts. Cocultured medium with rhBMP-2 also contained increased levels of matrix metalloproteinases. rhBMP-2-treated cocultured fibroblasts did not show a prominent difference in mRNA expression profile. Some cytokines, however, were detected in the conditioned medium by a human cytokine antibody array. Among them, the cancer invasion-related factor CCL5 was quantified by ELISA. Interestingly, CCL5 neutralizing antibodies significantly reduced the invasion of oral cancer cells. In conclusion, our results suggest that 1 ng/ml rhBMP-2 may induce invasion of oral squamous cell carcinoma (OSCC) cells by CCL5 release in coculture models. Therefore, we propose that a careful clinical examination before the use of rhBMP-2-containing biomaterials is indispensable for using rhBMP-2 treatment to prevent cancer progression.http://europepmc.org/articles/PMC4182698?pdf=render |
| spellingShingle | Mi-joo Kim Kwang-mahn Kim Jin Kim Kyoung-nam Kim BMP-2 promotes oral squamous carcinoma cell invasion by inducing CCL5 release. PLoS ONE |
| title | BMP-2 promotes oral squamous carcinoma cell invasion by inducing CCL5 release. |
| title_full | BMP-2 promotes oral squamous carcinoma cell invasion by inducing CCL5 release. |
| title_fullStr | BMP-2 promotes oral squamous carcinoma cell invasion by inducing CCL5 release. |
| title_full_unstemmed | BMP-2 promotes oral squamous carcinoma cell invasion by inducing CCL5 release. |
| title_short | BMP-2 promotes oral squamous carcinoma cell invasion by inducing CCL5 release. |
| title_sort | bmp 2 promotes oral squamous carcinoma cell invasion by inducing ccl5 release |
| url | http://europepmc.org/articles/PMC4182698?pdf=render |
| work_keys_str_mv | AT mijookim bmp2promotesoralsquamouscarcinomacellinvasionbyinducingccl5release AT kwangmahnkim bmp2promotesoralsquamouscarcinomacellinvasionbyinducingccl5release AT jinkim bmp2promotesoralsquamouscarcinomacellinvasionbyinducingccl5release AT kyoungnamkim bmp2promotesoralsquamouscarcinomacellinvasionbyinducingccl5release |