Macroepidemiological aspects of porcine reproductive and respiratory syndrome virus detection by major United States veterinary diagnostic laboratories over time, age group, and specimen.
This project investigates the macroepidemiological aspects of porcine reproductive and respiratory syndrome virus (PRRSV) RNA detection by veterinary diagnostic laboratories (VDLs) for the period 2007 through 2018. Standardized submission data and PRRSV real-time reverse-transcriptase polymerase cha...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0223544 |
| _version_ | 1818925459695468544 |
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| author | Giovani Trevisan Leticia C M Linhares Bret Crim Poonam Dubey Kent J Schwartz Eric R Burrough Rodger G Main Paul Sundberg Mary Thurn Paulo T F Lages Cesar A Corzo Jerry Torrison Jamie Henningson Eric Herrman Gregg A Hanzlicek Ram Raghavan Douglas Marthaler Jon Greseth Travis Clement Jane Christopher-Hennings Daniel C L Linhares |
| author_facet | Giovani Trevisan Leticia C M Linhares Bret Crim Poonam Dubey Kent J Schwartz Eric R Burrough Rodger G Main Paul Sundberg Mary Thurn Paulo T F Lages Cesar A Corzo Jerry Torrison Jamie Henningson Eric Herrman Gregg A Hanzlicek Ram Raghavan Douglas Marthaler Jon Greseth Travis Clement Jane Christopher-Hennings Daniel C L Linhares |
| author_sort | Giovani Trevisan |
| collection | DOAJ |
| description | This project investigates the macroepidemiological aspects of porcine reproductive and respiratory syndrome virus (PRRSV) RNA detection by veterinary diagnostic laboratories (VDLs) for the period 2007 through 2018. Standardized submission data and PRRSV real-time reverse-transcriptase polymerase chain reaction (RT-qPCR) test results from porcine samples were retrieved from four VDLs representing 95% of all swine samples tested in NAHLN laboratories in the US. Anonymized data were retrieved and organized at the case level using SAS (SAS® Version 9.4, SAS® Institute, Inc., Cary, NC) with the use of PROC DATA, PROC MERGE, and PROC SQL scripts. The final aggregated and anonymized dataset comprised of 547,873 unique cases was uploaded to Power Business Intelligence-Power BI® (Microsoft Corporation, Redmond, Washington) to construct dynamic charts. The number of cases tested for PRRSV doubled from 2010 to 2018, with that increase mainly driven by samples typically used for monitoring purposes rather than diagnosis of disease. Apparent seasonal trends for the frequency of PRRSV detection were consistently observed with a higher percentage of positive cases occurring during fall or winter months and lower during summer months, perhaps due to increased testing associated with well-known seasonal occurrence of swine respiratory disease. PRRSV type 2, also known as North American genotype, accounted for 94.76% of all positive cases and was distributed across the US. PRRSV type 1, also known as European genotype, was geographically restricted and accounted for 2.15% of all positive cases. Co-detection of both strains accounted for 3.09% of the positive cases. Both oral fluid and processing fluid samples, had a rapid increase in the number of submissions soon after they were described in 2008 and 2017, respectively, suggesting rapid adoption of these specimens by the US swine industry for PRRSV monitoring in swine populations. As part of this project, a bio-informatics tool defined as Swine Disease Reporting System (SDRS) was developed. This tool has real-time capability to inform the US swine industry on the macroepidemiological aspects of PRRSV detection, and is easily adaptable for other analytes relevant to the swine industry. |
| first_indexed | 2024-12-20T02:41:34Z |
| format | Article |
| id | doaj.art-9131dc2f42194fc0a167008b943f0d15 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T02:41:34Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9131dc2f42194fc0a167008b943f0d152022-12-21T19:56:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011410e022354410.1371/journal.pone.0223544Macroepidemiological aspects of porcine reproductive and respiratory syndrome virus detection by major United States veterinary diagnostic laboratories over time, age group, and specimen.Giovani TrevisanLeticia C M LinharesBret CrimPoonam DubeyKent J SchwartzEric R BurroughRodger G MainPaul SundbergMary ThurnPaulo T F LagesCesar A CorzoJerry TorrisonJamie HenningsonEric HerrmanGregg A HanzlicekRam RaghavanDouglas MarthalerJon GresethTravis ClementJane Christopher-HenningsDaniel C L LinharesThis project investigates the macroepidemiological aspects of porcine reproductive and respiratory syndrome virus (PRRSV) RNA detection by veterinary diagnostic laboratories (VDLs) for the period 2007 through 2018. Standardized submission data and PRRSV real-time reverse-transcriptase polymerase chain reaction (RT-qPCR) test results from porcine samples were retrieved from four VDLs representing 95% of all swine samples tested in NAHLN laboratories in the US. Anonymized data were retrieved and organized at the case level using SAS (SAS® Version 9.4, SAS® Institute, Inc., Cary, NC) with the use of PROC DATA, PROC MERGE, and PROC SQL scripts. The final aggregated and anonymized dataset comprised of 547,873 unique cases was uploaded to Power Business Intelligence-Power BI® (Microsoft Corporation, Redmond, Washington) to construct dynamic charts. The number of cases tested for PRRSV doubled from 2010 to 2018, with that increase mainly driven by samples typically used for monitoring purposes rather than diagnosis of disease. Apparent seasonal trends for the frequency of PRRSV detection were consistently observed with a higher percentage of positive cases occurring during fall or winter months and lower during summer months, perhaps due to increased testing associated with well-known seasonal occurrence of swine respiratory disease. PRRSV type 2, also known as North American genotype, accounted for 94.76% of all positive cases and was distributed across the US. PRRSV type 1, also known as European genotype, was geographically restricted and accounted for 2.15% of all positive cases. Co-detection of both strains accounted for 3.09% of the positive cases. Both oral fluid and processing fluid samples, had a rapid increase in the number of submissions soon after they were described in 2008 and 2017, respectively, suggesting rapid adoption of these specimens by the US swine industry for PRRSV monitoring in swine populations. As part of this project, a bio-informatics tool defined as Swine Disease Reporting System (SDRS) was developed. This tool has real-time capability to inform the US swine industry on the macroepidemiological aspects of PRRSV detection, and is easily adaptable for other analytes relevant to the swine industry.https://doi.org/10.1371/journal.pone.0223544 |
| spellingShingle | Giovani Trevisan Leticia C M Linhares Bret Crim Poonam Dubey Kent J Schwartz Eric R Burrough Rodger G Main Paul Sundberg Mary Thurn Paulo T F Lages Cesar A Corzo Jerry Torrison Jamie Henningson Eric Herrman Gregg A Hanzlicek Ram Raghavan Douglas Marthaler Jon Greseth Travis Clement Jane Christopher-Hennings Daniel C L Linhares Macroepidemiological aspects of porcine reproductive and respiratory syndrome virus detection by major United States veterinary diagnostic laboratories over time, age group, and specimen. PLoS ONE |
| title | Macroepidemiological aspects of porcine reproductive and respiratory syndrome virus detection by major United States veterinary diagnostic laboratories over time, age group, and specimen. |
| title_full | Macroepidemiological aspects of porcine reproductive and respiratory syndrome virus detection by major United States veterinary diagnostic laboratories over time, age group, and specimen. |
| title_fullStr | Macroepidemiological aspects of porcine reproductive and respiratory syndrome virus detection by major United States veterinary diagnostic laboratories over time, age group, and specimen. |
| title_full_unstemmed | Macroepidemiological aspects of porcine reproductive and respiratory syndrome virus detection by major United States veterinary diagnostic laboratories over time, age group, and specimen. |
| title_short | Macroepidemiological aspects of porcine reproductive and respiratory syndrome virus detection by major United States veterinary diagnostic laboratories over time, age group, and specimen. |
| title_sort | macroepidemiological aspects of porcine reproductive and respiratory syndrome virus detection by major united states veterinary diagnostic laboratories over time age group and specimen |
| url | https://doi.org/10.1371/journal.pone.0223544 |
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