Sustainable bacterial cellulose production by Achromobacter using mango peel waste
Abstract Bacterial Cellulose (BC) is still the most renewable available biopolymer produced in fine nature from alternative microbial sources as bacteria. In the present study, newly BC producing bacteria were successfully isolated from acidic fruits. The most potent producer was isolated from straw...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-023-02031-3 |
| _version_ | 1811165643672649728 |
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| author | Mohamed S. Hasanin Mohamed Abdelraof Amr H. Hashem Houssni El Saied |
| author_facet | Mohamed S. Hasanin Mohamed Abdelraof Amr H. Hashem Houssni El Saied |
| author_sort | Mohamed S. Hasanin |
| collection | DOAJ |
| description | Abstract Bacterial Cellulose (BC) is still the most renewable available biopolymer produced in fine nature from alternative microbial sources as bacteria. In the present study, newly BC producing bacteria were successfully isolated from acidic fruits. The most potent producer was isolated from strawberry and identified genetically using 16 s rRNA technique as Achromobacter S3. Different fruit peels were screened to produce BC using the cheapest culture medium. Among them, Mango peel waste (MPW) hydrolysate proved to be the significant inducible alternative medium without any extra nutrients for the maximum productivity. Improvement of the BC yield was successfully achieved via statistical optimization of the MPW culture medium, from 0.52 g/L to 1.22 g/L with 2.5-fold increased about the standard HS culture medium. Additionally, the physicochemical analysis affirmed the cellulose molecular structure as well as observed the crystallinity of nanofiber as 72 and 79% for BC produced by Achromobacter S33 on HS and MPW media, respectively. Moreover, the topographical study illustrated that the BC nanofibers had close characteristics upon fiber dimeter and length as about 10 and 200 nm, respectively. |
| first_indexed | 2024-04-10T15:40:00Z |
| format | Article |
| id | doaj.art-9136a31de07b4eba800a5590259329da |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-10T15:40:00Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-9136a31de07b4eba800a5590259329da2023-02-12T12:26:29ZengBMCMicrobial Cell Factories1475-28592023-02-0122111210.1186/s12934-023-02031-3Sustainable bacterial cellulose production by Achromobacter using mango peel wasteMohamed S. Hasanin0Mohamed Abdelraof1Amr H. Hashem2Houssni El Saied3Cellulose and Paper Department, National Research CentreMicrobial Chemistry Department, National Research CentreBotany and Microbiology Department, Faculty of Science, Al-Azhar UniversityCellulose and Paper Department, National Research CentreAbstract Bacterial Cellulose (BC) is still the most renewable available biopolymer produced in fine nature from alternative microbial sources as bacteria. In the present study, newly BC producing bacteria were successfully isolated from acidic fruits. The most potent producer was isolated from strawberry and identified genetically using 16 s rRNA technique as Achromobacter S3. Different fruit peels were screened to produce BC using the cheapest culture medium. Among them, Mango peel waste (MPW) hydrolysate proved to be the significant inducible alternative medium without any extra nutrients for the maximum productivity. Improvement of the BC yield was successfully achieved via statistical optimization of the MPW culture medium, from 0.52 g/L to 1.22 g/L with 2.5-fold increased about the standard HS culture medium. Additionally, the physicochemical analysis affirmed the cellulose molecular structure as well as observed the crystallinity of nanofiber as 72 and 79% for BC produced by Achromobacter S33 on HS and MPW media, respectively. Moreover, the topographical study illustrated that the BC nanofibers had close characteristics upon fiber dimeter and length as about 10 and 200 nm, respectively.https://doi.org/10.1186/s12934-023-02031-3Bacterial celluloseAchromobacterAgricultural wastesOptimization |
| spellingShingle | Mohamed S. Hasanin Mohamed Abdelraof Amr H. Hashem Houssni El Saied Sustainable bacterial cellulose production by Achromobacter using mango peel waste Microbial Cell Factories Bacterial cellulose Achromobacter Agricultural wastes Optimization |
| title | Sustainable bacterial cellulose production by Achromobacter using mango peel waste |
| title_full | Sustainable bacterial cellulose production by Achromobacter using mango peel waste |
| title_fullStr | Sustainable bacterial cellulose production by Achromobacter using mango peel waste |
| title_full_unstemmed | Sustainable bacterial cellulose production by Achromobacter using mango peel waste |
| title_short | Sustainable bacterial cellulose production by Achromobacter using mango peel waste |
| title_sort | sustainable bacterial cellulose production by achromobacter using mango peel waste |
| topic | Bacterial cellulose Achromobacter Agricultural wastes Optimization |
| url | https://doi.org/10.1186/s12934-023-02031-3 |
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