Transplantation of neuron‐inducing grafts embedding positively charged gold nanoparticles for the treatment of spinal cord injury
Abstract In this study, we aimed to investigate the recovery after traumatic spinal cord injury (SCI) by inducing cellular differentiation of transplanted neural stem cells (NSCs) into neurons. We dissociated NSCs from the spinal cords of Fisher 344 rat embryos. An injectable gel crosslinked with gl...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-09-01
|
| Series: | Bioengineering & Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/btm2.10326 |
| _version_ | 1797995037066264576 |
|---|---|
| author | Wan‐Kyu Ko Seong Jun Kim Gong Ho Han Daye Lee Dabin Jeong Sang Jin Lee In‐Bo Han Je Beom Hong Seung Hun Sheen Seil Sohn |
| author_facet | Wan‐Kyu Ko Seong Jun Kim Gong Ho Han Daye Lee Dabin Jeong Sang Jin Lee In‐Bo Han Je Beom Hong Seung Hun Sheen Seil Sohn |
| author_sort | Wan‐Kyu Ko |
| collection | DOAJ |
| description | Abstract In this study, we aimed to investigate the recovery after traumatic spinal cord injury (SCI) by inducing cellular differentiation of transplanted neural stem cells (NSCs) into neurons. We dissociated NSCs from the spinal cords of Fisher 344 rat embryos. An injectable gel crosslinked with glycol chitosan and oxidized hyaluronate was used as a vehicle for NSC transplantation. The gel graft containing the NSC and positively charged gold nanoparticles (pGNP) was implanted into spinal cord lesions in Sprague–Dawley rats (NSC‐pGNP gel group). Cellular differentiation of grafted NSCs into neurons (stained with β‐tubulin III [also called Tuj1]) was significantly increased in the NSC‐pGNP gel group (***p < 0.001) compared to those of two control groups (NSC and NSC gel groups) in the SCI conditions. The NSC‐pGNP gel group showed the lowest differentiation into astrocytes (stained with glial fibrillary acidic protein). Regeneration of damaged axons (stained with biotinylated dextran amines) within the lesion was two‐fold higher in the NSC‐pGNP gel group than that in the NSC gel group. The highest locomotor scores were also found in the NSC‐pGNP gel group. These outcomes suggest that neuron‐inducing pGNP gel graft embedding embryonic spinal cord‐derived NSCs can be a useful type of stem cell therapy after SCI. |
| first_indexed | 2024-04-11T09:55:11Z |
| format | Article |
| id | doaj.art-9136de7be8d14558b913f0f70669a5da |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-04-11T09:55:11Z |
| publishDate | 2022-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-9136de7be8d14558b913f0f70669a5da2022-12-22T04:30:37ZengWileyBioengineering & Translational Medicine2380-67612022-09-0173n/an/a10.1002/btm2.10326Transplantation of neuron‐inducing grafts embedding positively charged gold nanoparticles for the treatment of spinal cord injuryWan‐Kyu Ko0Seong Jun Kim1Gong Ho Han2Daye Lee3Dabin Jeong4Sang Jin Lee5In‐Bo Han6Je Beom Hong7Seung Hun Sheen8Seil Sohn9Department of Neurosurgery CHA Bundang Medical Center, CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaDepartment of Neurosurgery CHA Bundang Medical Center, CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaDepartment of Neurosurgery CHA Bundang Medical Center, CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaDepartment of Neurosurgery CHA Bundang Medical Center, CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaDepartment of Neurosurgery CHA Bundang Medical Center, CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaDepartment of Dental Materials, School of Dentistry Kyung Hee University Seoul Republic of KoreaDepartment of Neurosurgery CHA Bundang Medical Center, CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaDepartment of Neurosurgery Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine Seoul Republic of KoreaDepartment of Neurosurgery CHA Bundang Medical Center, CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaDepartment of Neurosurgery CHA Bundang Medical Center, CHA University Seongnam‐si Gyeonggi‐do Republic of KoreaAbstract In this study, we aimed to investigate the recovery after traumatic spinal cord injury (SCI) by inducing cellular differentiation of transplanted neural stem cells (NSCs) into neurons. We dissociated NSCs from the spinal cords of Fisher 344 rat embryos. An injectable gel crosslinked with glycol chitosan and oxidized hyaluronate was used as a vehicle for NSC transplantation. The gel graft containing the NSC and positively charged gold nanoparticles (pGNP) was implanted into spinal cord lesions in Sprague–Dawley rats (NSC‐pGNP gel group). Cellular differentiation of grafted NSCs into neurons (stained with β‐tubulin III [also called Tuj1]) was significantly increased in the NSC‐pGNP gel group (***p < 0.001) compared to those of two control groups (NSC and NSC gel groups) in the SCI conditions. The NSC‐pGNP gel group showed the lowest differentiation into astrocytes (stained with glial fibrillary acidic protein). Regeneration of damaged axons (stained with biotinylated dextran amines) within the lesion was two‐fold higher in the NSC‐pGNP gel group than that in the NSC gel group. The highest locomotor scores were also found in the NSC‐pGNP gel group. These outcomes suggest that neuron‐inducing pGNP gel graft embedding embryonic spinal cord‐derived NSCs can be a useful type of stem cell therapy after SCI.https://doi.org/10.1002/btm2.10326astrocytegold nanoparticleneural stem cellneuronspinal cord injury |
| spellingShingle | Wan‐Kyu Ko Seong Jun Kim Gong Ho Han Daye Lee Dabin Jeong Sang Jin Lee In‐Bo Han Je Beom Hong Seung Hun Sheen Seil Sohn Transplantation of neuron‐inducing grafts embedding positively charged gold nanoparticles for the treatment of spinal cord injury Bioengineering & Translational Medicine astrocyte gold nanoparticle neural stem cell neuron spinal cord injury |
| title | Transplantation of neuron‐inducing grafts embedding positively charged gold nanoparticles for the treatment of spinal cord injury |
| title_full | Transplantation of neuron‐inducing grafts embedding positively charged gold nanoparticles for the treatment of spinal cord injury |
| title_fullStr | Transplantation of neuron‐inducing grafts embedding positively charged gold nanoparticles for the treatment of spinal cord injury |
| title_full_unstemmed | Transplantation of neuron‐inducing grafts embedding positively charged gold nanoparticles for the treatment of spinal cord injury |
| title_short | Transplantation of neuron‐inducing grafts embedding positively charged gold nanoparticles for the treatment of spinal cord injury |
| title_sort | transplantation of neuron inducing grafts embedding positively charged gold nanoparticles for the treatment of spinal cord injury |
| topic | astrocyte gold nanoparticle neural stem cell neuron spinal cord injury |
| url | https://doi.org/10.1002/btm2.10326 |
| work_keys_str_mv | AT wankyuko transplantationofneuroninducinggraftsembeddingpositivelychargedgoldnanoparticlesforthetreatmentofspinalcordinjury AT seongjunkim transplantationofneuroninducinggraftsembeddingpositivelychargedgoldnanoparticlesforthetreatmentofspinalcordinjury AT gonghohan transplantationofneuroninducinggraftsembeddingpositivelychargedgoldnanoparticlesforthetreatmentofspinalcordinjury AT dayelee transplantationofneuroninducinggraftsembeddingpositivelychargedgoldnanoparticlesforthetreatmentofspinalcordinjury AT dabinjeong transplantationofneuroninducinggraftsembeddingpositivelychargedgoldnanoparticlesforthetreatmentofspinalcordinjury AT sangjinlee transplantationofneuroninducinggraftsembeddingpositivelychargedgoldnanoparticlesforthetreatmentofspinalcordinjury AT inbohan transplantationofneuroninducinggraftsembeddingpositivelychargedgoldnanoparticlesforthetreatmentofspinalcordinjury AT jebeomhong transplantationofneuroninducinggraftsembeddingpositivelychargedgoldnanoparticlesforthetreatmentofspinalcordinjury AT seunghunsheen transplantationofneuroninducinggraftsembeddingpositivelychargedgoldnanoparticlesforthetreatmentofspinalcordinjury AT seilsohn transplantationofneuroninducinggraftsembeddingpositivelychargedgoldnanoparticlesforthetreatmentofspinalcordinjury |