Biosynthesized copper oxide nanoparticles (CuO NPs) enhances the anti-biofilm efficacy against K. pneumoniae and S. aureus
In this experimental study was designed to synthesis the CuO NPs using medicinal plant of Moringa oleifera for eradicate the biofilm formation. In result, the UV-spectrum of the synthesized CuO NPs subjected to the range between 200 and 600 nm. Among these, the prominent absorbance peak at 430 nm in...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722003019 |
| _version_ | 1811258001504337920 |
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| author | Boliang Bai Sivakumar Saranya Vaitheeswaran Dheepaasri S. Muniasamy Naiyf S. Alharbi Barathi Selvaraj Vinod S. Undal Balasubramanian Mythili Gnanamangai |
| author_facet | Boliang Bai Sivakumar Saranya Vaitheeswaran Dheepaasri S. Muniasamy Naiyf S. Alharbi Barathi Selvaraj Vinod S. Undal Balasubramanian Mythili Gnanamangai |
| author_sort | Boliang Bai |
| collection | DOAJ |
| description | In this experimental study was designed to synthesis the CuO NPs using medicinal plant of Moringa oleifera for eradicate the biofilm formation. In result, the UV-spectrum of the synthesized CuO NPs subjected to the range between 200 and 600 nm. Among these, the prominent absorbance peak at 430 nm indicated the peak was held to the copper oxide nanoparticle (CuO NPs). The particle size of biosynthesized CuO NPs varied from 90 nm to 250 nm with more than 50% distribution appearing between 130 nm and 170 nm. In addition, it was further confirmed by PSA, SEM, TEM and SAED of irregular shape and also crystalline nature. All the synthesis and characterization results were clearly supported to the result and confirmed that the synthesized nanoparticle is CuO NPs. Further, the biosynthesized CuO NPs was performed against biofilm forming K. pneumoniae, S. aureus and A. baumannii. The anti-bacterial activity result was shown with 20 mm, 14 mm and 18 mm zone of inhibition against K. pneumoniae, S. aureus and A. baumannii. The minimum biofilm inhibition concentration result was more evident, that the CuO NPs has excellent anti-biofilm activity at 1000 µg/mL concentration. Furthermore, the decreased survival rate of the CuO NPs was observed at 1000 µg /mL concentration by liquid survival degradation assay. Overall, all the results were clearly confirmed that the CuO NPs has excellent anti-biofilm ability against K. pneumoniae, S. aureus and A. baumannii. |
| first_indexed | 2024-04-12T18:06:24Z |
| format | Article |
| id | doaj.art-91374a05bf614fed88eed267bf9108e3 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-12T18:06:24Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-91374a05bf614fed88eed267bf9108e32022-12-22T03:21:58ZengElsevierJournal of King Saud University: Science1018-36472022-08-01346102120Biosynthesized copper oxide nanoparticles (CuO NPs) enhances the anti-biofilm efficacy against K. pneumoniae and S. aureusBoliang Bai0Sivakumar Saranya1Vaitheeswaran Dheepaasri2S. Muniasamy3Naiyf S. Alharbi4Barathi Selvaraj5Vinod S. Undal6Balasubramanian Mythili Gnanamangai7Department of Neonatology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, PR ChinaDepartment of Biotechnology, K.S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu, IndiaDepartment of Biotechnology, K.S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu, IndiaDepartment of Microbiology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi 626 124, Virudhunagar District, Tamil Nadu, IndiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Biotechnology, School of Chemical Engineering, Yeungnam University, Gyeongsangbuk-do, South KoreaDepartment of Botany, Ghulam Nabi Azad College, Barshitakali Dist Akola, 444401 Maharashtra, IndiaDepartment of Biotechnology, K.S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu, India; Corresponding author.In this experimental study was designed to synthesis the CuO NPs using medicinal plant of Moringa oleifera for eradicate the biofilm formation. In result, the UV-spectrum of the synthesized CuO NPs subjected to the range between 200 and 600 nm. Among these, the prominent absorbance peak at 430 nm indicated the peak was held to the copper oxide nanoparticle (CuO NPs). The particle size of biosynthesized CuO NPs varied from 90 nm to 250 nm with more than 50% distribution appearing between 130 nm and 170 nm. In addition, it was further confirmed by PSA, SEM, TEM and SAED of irregular shape and also crystalline nature. All the synthesis and characterization results were clearly supported to the result and confirmed that the synthesized nanoparticle is CuO NPs. Further, the biosynthesized CuO NPs was performed against biofilm forming K. pneumoniae, S. aureus and A. baumannii. The anti-bacterial activity result was shown with 20 mm, 14 mm and 18 mm zone of inhibition against K. pneumoniae, S. aureus and A. baumannii. The minimum biofilm inhibition concentration result was more evident, that the CuO NPs has excellent anti-biofilm activity at 1000 µg/mL concentration. Furthermore, the decreased survival rate of the CuO NPs was observed at 1000 µg /mL concentration by liquid survival degradation assay. Overall, all the results were clearly confirmed that the CuO NPs has excellent anti-biofilm ability against K. pneumoniae, S. aureus and A. baumannii.http://www.sciencedirect.com/science/article/pii/S1018364722003019Copper oxide nanoparticlesGreen synthesisAnti-biofilm activityMinimum biofilm inhibition concentrationBiofilm survival assay |
| spellingShingle | Boliang Bai Sivakumar Saranya Vaitheeswaran Dheepaasri S. Muniasamy Naiyf S. Alharbi Barathi Selvaraj Vinod S. Undal Balasubramanian Mythili Gnanamangai Biosynthesized copper oxide nanoparticles (CuO NPs) enhances the anti-biofilm efficacy against K. pneumoniae and S. aureus Journal of King Saud University: Science Copper oxide nanoparticles Green synthesis Anti-biofilm activity Minimum biofilm inhibition concentration Biofilm survival assay |
| title | Biosynthesized copper oxide nanoparticles (CuO NPs) enhances the anti-biofilm efficacy against K. pneumoniae and S. aureus |
| title_full | Biosynthesized copper oxide nanoparticles (CuO NPs) enhances the anti-biofilm efficacy against K. pneumoniae and S. aureus |
| title_fullStr | Biosynthesized copper oxide nanoparticles (CuO NPs) enhances the anti-biofilm efficacy against K. pneumoniae and S. aureus |
| title_full_unstemmed | Biosynthesized copper oxide nanoparticles (CuO NPs) enhances the anti-biofilm efficacy against K. pneumoniae and S. aureus |
| title_short | Biosynthesized copper oxide nanoparticles (CuO NPs) enhances the anti-biofilm efficacy against K. pneumoniae and S. aureus |
| title_sort | biosynthesized copper oxide nanoparticles cuo nps enhances the anti biofilm efficacy against k pneumoniae and s aureus |
| topic | Copper oxide nanoparticles Green synthesis Anti-biofilm activity Minimum biofilm inhibition concentration Biofilm survival assay |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722003019 |
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