Characterization of <i>Pipistrellus pygmaeus</i> Bat Virome from Sweden
Increasing amounts of data indicate that bats harbor a higher viral diversity relative to other mammalian orders, and they have been recognized as potential reservoirs for pathogenic viruses, such as the Hendra, Nipah, Marburg, and SARS-CoV viruses. Here, we present the first viral metagenomic analy...
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| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/8/1654 |
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| author | Harindranath Cholleti Johnny de Jong Anne-Lie Blomström Mikael Berg |
| author_facet | Harindranath Cholleti Johnny de Jong Anne-Lie Blomström Mikael Berg |
| author_sort | Harindranath Cholleti |
| collection | DOAJ |
| description | Increasing amounts of data indicate that bats harbor a higher viral diversity relative to other mammalian orders, and they have been recognized as potential reservoirs for pathogenic viruses, such as the Hendra, Nipah, Marburg, and SARS-CoV viruses. Here, we present the first viral metagenomic analysis of <i>Pipistrellus pygmaeus</i> from Uppsala, Sweden. Total RNA was extracted from the saliva and feces of individual bats and analyzed using Illumina sequencing. The results identified sequences related to 51 different viral families, including vertebrate, invertebrate, and plant viruses. These viral families include <i>Coronaviridae, Picornaviridae, Dicistroviridae, Astroviridae, Hepeviridae, Reoviridae, Botourmiaviridae, Lispviridae, Totiviridae, Botoumiaviridae, Parvoviridae, Retroviridae, Adenoviridae</i>, and <i>Partitiviridae,</i> as well as different unclassified viruses. We further characterized three near full-length genome sequences of bat coronaviruses. A phylogenetic analysis showed that these belonged to alphacoronaviruses with the closest similarity (78–99% at the protein level) to Danish and Finnish bat coronaviruses detected in <i>Pipistrellus</i> and <i>Myotis</i> bats. In addition, the full-length and the near full-length genomes of picornavirus were characterized. These showed the closest similarity (88–94% at the protein level) to bat picornaviruses identified in Chinese bats. Altogether, the results of this study show that Swedish <i>Pipistrellus</i> bats harbor a great diversity of viruses, some of which are closely related to mammalian viruses. This study expands our knowledge on the bat population virome and improves our understanding of the evolution and transmission of viruses among bats and to other species. |
| first_indexed | 2024-03-09T12:22:57Z |
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| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:22:57Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Viruses |
| spelling | doaj.art-913815f47a1044f1a8dc34b9f94e99e62023-11-30T22:38:28ZengMDPI AGViruses1999-49152022-07-01148165410.3390/v14081654Characterization of <i>Pipistrellus pygmaeus</i> Bat Virome from SwedenHarindranath Cholleti0Johnny de Jong1Anne-Lie Blomström2Mikael Berg3Section of Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences (SLU), P.O. Box 7028, 750 07 Uppsala, SwedenSwedish Biodiversity Centre (CBM), Department of Urban and Rural Development, Swedish University of Agricultural Sciences (SLU), P.O. Box 7016, 750 07 Uppsala, SwedenSection of Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences (SLU), P.O. Box 7028, 750 07 Uppsala, SwedenSection of Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences (SLU), P.O. Box 7028, 750 07 Uppsala, SwedenIncreasing amounts of data indicate that bats harbor a higher viral diversity relative to other mammalian orders, and they have been recognized as potential reservoirs for pathogenic viruses, such as the Hendra, Nipah, Marburg, and SARS-CoV viruses. Here, we present the first viral metagenomic analysis of <i>Pipistrellus pygmaeus</i> from Uppsala, Sweden. Total RNA was extracted from the saliva and feces of individual bats and analyzed using Illumina sequencing. The results identified sequences related to 51 different viral families, including vertebrate, invertebrate, and plant viruses. These viral families include <i>Coronaviridae, Picornaviridae, Dicistroviridae, Astroviridae, Hepeviridae, Reoviridae, Botourmiaviridae, Lispviridae, Totiviridae, Botoumiaviridae, Parvoviridae, Retroviridae, Adenoviridae</i>, and <i>Partitiviridae,</i> as well as different unclassified viruses. We further characterized three near full-length genome sequences of bat coronaviruses. A phylogenetic analysis showed that these belonged to alphacoronaviruses with the closest similarity (78–99% at the protein level) to Danish and Finnish bat coronaviruses detected in <i>Pipistrellus</i> and <i>Myotis</i> bats. In addition, the full-length and the near full-length genomes of picornavirus were characterized. These showed the closest similarity (88–94% at the protein level) to bat picornaviruses identified in Chinese bats. Altogether, the results of this study show that Swedish <i>Pipistrellus</i> bats harbor a great diversity of viruses, some of which are closely related to mammalian viruses. This study expands our knowledge on the bat population virome and improves our understanding of the evolution and transmission of viruses among bats and to other species.https://www.mdpi.com/1999-4915/14/8/1654batmetagenomicsviromecoronavirusSweden |
| spellingShingle | Harindranath Cholleti Johnny de Jong Anne-Lie Blomström Mikael Berg Characterization of <i>Pipistrellus pygmaeus</i> Bat Virome from Sweden Viruses bat metagenomics virome coronavirus Sweden |
| title | Characterization of <i>Pipistrellus pygmaeus</i> Bat Virome from Sweden |
| title_full | Characterization of <i>Pipistrellus pygmaeus</i> Bat Virome from Sweden |
| title_fullStr | Characterization of <i>Pipistrellus pygmaeus</i> Bat Virome from Sweden |
| title_full_unstemmed | Characterization of <i>Pipistrellus pygmaeus</i> Bat Virome from Sweden |
| title_short | Characterization of <i>Pipistrellus pygmaeus</i> Bat Virome from Sweden |
| title_sort | characterization of i pipistrellus pygmaeus i bat virome from sweden |
| topic | bat metagenomics virome coronavirus Sweden |
| url | https://www.mdpi.com/1999-4915/14/8/1654 |
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