Alterations to the Lung Microbiome in Idiopathic Pulmonary Fibrosis Patients
Lung microbiome ecosystem homeostasis in idiopathic pulmonary fibrosis (IPF) remains uncharacterized. The aims of this study were to identify unique microbial signatures of the lung microbiome and analyze microbial gene function in IPF patients. DNA isolated from BALF samples was obtained for high-t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2019.00149/full |
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| author | Xunliang Tong Fei Su Xiaomao Xu Hongtao Xu Ting Yang Ting Yang Qixia Xu Huaping Dai Huaping Dai Kewu Huang Lihui Zou Wenna Zhang Surui Pei Fei Xiao Fei Xiao Yanming Li Chen Wang Chen Wang Chen Wang |
| author_facet | Xunliang Tong Fei Su Xiaomao Xu Hongtao Xu Ting Yang Ting Yang Qixia Xu Huaping Dai Huaping Dai Kewu Huang Lihui Zou Wenna Zhang Surui Pei Fei Xiao Fei Xiao Yanming Li Chen Wang Chen Wang Chen Wang |
| author_sort | Xunliang Tong |
| collection | DOAJ |
| description | Lung microbiome ecosystem homeostasis in idiopathic pulmonary fibrosis (IPF) remains uncharacterized. The aims of this study were to identify unique microbial signatures of the lung microbiome and analyze microbial gene function in IPF patients. DNA isolated from BALF samples was obtained for high-throughput gene sequencing. Microbial metagenomic data were used for principal component analysis (PCA) and analyzed at different taxonomic levels. Shotgun metagenomic data were annotated using the KEGG database and were analyzed for functional and metabolic pathways. In this study, 17 IPF patients and 38 healthy subjects (smokers and non-smokers) were recruited. For the PCA, the first and the second principal component explained 16.3 and 13.4% of the overall variability, respectively. The β diversity of microbiome was reduced in the IPF group. Signature of IPF's microbes was enriched of Streptococcus, Pseudobutyrivibrio, and Anaerorhabdus. The translocation of lung microbiome was shown that 32.84% of them were from oral. After analysis of gene function, ABC transporter systems, biofilm formation, and two-component regulatory system were enriched in IPF patients' microbiome. Here we shown the microbiology characteristics in IPF patients. The microbiome may participate in altering internal conditions and involving in generating antibiotic resistance in IPF patients. |
| first_indexed | 2024-12-10T15:47:55Z |
| format | Article |
| id | doaj.art-913b3c1a1b61432aac40b922cadbcd81 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-10T15:47:55Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-913b3c1a1b61432aac40b922cadbcd812022-12-22T01:42:54ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882019-05-01910.3389/fcimb.2019.00149437323Alterations to the Lung Microbiome in Idiopathic Pulmonary Fibrosis PatientsXunliang Tong0Fei Su1Xiaomao Xu2Hongtao Xu3Ting Yang4Ting Yang5Qixia Xu6Huaping Dai7Huaping Dai8Kewu Huang9Lihui Zou10Wenna Zhang11Surui Pei12Fei Xiao13Fei Xiao14Yanming Li15Chen Wang16Chen Wang17Chen Wang18Department of Respiratory and Critical Care Medicine, National Center of Gerontology, Beijing Hospital, Beijing, ChinaClinical Biobank, National Center of Gerontology, Beijing Hospital, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, National Center of Gerontology, Beijing Hospital, Beijing, ChinaDepartment of Laboratory Medicine, Beijing Hospital, Beijing, ChinaNational Clinical Research Center for Respiratory Diseases, Center for Respiratory Diseases, China-Japan Friendship Hospital, Peking University Health Science Center, Beijing, ChinaDepartment of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Peking University Health Science Center, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, Bengbu University Affiliated Hospital, Bengbu, ChinaNational Clinical Research Center for Respiratory Diseases, Center for Respiratory Diseases, China-Japan Friendship Hospital, Peking University Health Science Center, Beijing, ChinaDepartment of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Peking University Health Science Center, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University and Beijing Institute of Respiratory Medicine, Beijing, ChinaThe Key Laboratory of Geriatrics, National Center of Gerontology, Beijing Hospital, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, National Center of Gerontology, Beijing Hospital, Beijing, ChinaAnnoroad Gene Technology (Beijing) Co., Ltd., Beijing, ChinaClinical Biobank, National Center of Gerontology, Beijing Hospital, Beijing, ChinaThe Key Laboratory of Geriatrics, National Center of Gerontology, Beijing Hospital, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, National Center of Gerontology, Beijing Hospital, Beijing, ChinaNational Clinical Research Center for Respiratory Diseases, Center for Respiratory Diseases, China-Japan Friendship Hospital, Peking University Health Science Center, Beijing, ChinaDepartment of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Peking University Health Science Center, Beijing, China0National Clinical Research Center for Respiratory Diseases, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaLung microbiome ecosystem homeostasis in idiopathic pulmonary fibrosis (IPF) remains uncharacterized. The aims of this study were to identify unique microbial signatures of the lung microbiome and analyze microbial gene function in IPF patients. DNA isolated from BALF samples was obtained for high-throughput gene sequencing. Microbial metagenomic data were used for principal component analysis (PCA) and analyzed at different taxonomic levels. Shotgun metagenomic data were annotated using the KEGG database and were analyzed for functional and metabolic pathways. In this study, 17 IPF patients and 38 healthy subjects (smokers and non-smokers) were recruited. For the PCA, the first and the second principal component explained 16.3 and 13.4% of the overall variability, respectively. The β diversity of microbiome was reduced in the IPF group. Signature of IPF's microbes was enriched of Streptococcus, Pseudobutyrivibrio, and Anaerorhabdus. The translocation of lung microbiome was shown that 32.84% of them were from oral. After analysis of gene function, ABC transporter systems, biofilm formation, and two-component regulatory system were enriched in IPF patients' microbiome. Here we shown the microbiology characteristics in IPF patients. The microbiome may participate in altering internal conditions and involving in generating antibiotic resistance in IPF patients.https://www.frontiersin.org/article/10.3389/fcimb.2019.00149/fullidiopathic pulmonary fibrosisbronchoalveolar lavage fluidmicrobiotaantibiotic resistant genevirulence factor |
| spellingShingle | Xunliang Tong Fei Su Xiaomao Xu Hongtao Xu Ting Yang Ting Yang Qixia Xu Huaping Dai Huaping Dai Kewu Huang Lihui Zou Wenna Zhang Surui Pei Fei Xiao Fei Xiao Yanming Li Chen Wang Chen Wang Chen Wang Alterations to the Lung Microbiome in Idiopathic Pulmonary Fibrosis Patients Frontiers in Cellular and Infection Microbiology idiopathic pulmonary fibrosis bronchoalveolar lavage fluid microbiota antibiotic resistant gene virulence factor |
| title | Alterations to the Lung Microbiome in Idiopathic Pulmonary Fibrosis Patients |
| title_full | Alterations to the Lung Microbiome in Idiopathic Pulmonary Fibrosis Patients |
| title_fullStr | Alterations to the Lung Microbiome in Idiopathic Pulmonary Fibrosis Patients |
| title_full_unstemmed | Alterations to the Lung Microbiome in Idiopathic Pulmonary Fibrosis Patients |
| title_short | Alterations to the Lung Microbiome in Idiopathic Pulmonary Fibrosis Patients |
| title_sort | alterations to the lung microbiome in idiopathic pulmonary fibrosis patients |
| topic | idiopathic pulmonary fibrosis bronchoalveolar lavage fluid microbiota antibiotic resistant gene virulence factor |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2019.00149/full |
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