Isolation and molecular identification of Mycobacterium bovis in tissue lesions of cattle slaughtered in slaughterhouses located in the State of Ceará
ABSTRACT The objective of this research was to identify Mycobacterium bovis in lesions suggestive of tuberculosis in bovine carcasses in the State of Ceará, by means of bacteriological and molecular diagnostic tests. Between August 2017 and January 2019, the State inspection service (SIE) inspected...
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Universidade Federal de Minas Gerais
2022-05-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000200338&lng=en&tlng=en |
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| author | J.Q. Amorim B.R. Santos H.F. Fehlberg A.V. Silva F.F. Ferreira J.N. Costa F. Alzamora Filho |
| author_facet | J.Q. Amorim B.R. Santos H.F. Fehlberg A.V. Silva F.F. Ferreira J.N. Costa F. Alzamora Filho |
| author_sort | J.Q. Amorim |
| collection | DOAJ |
| description | ABSTRACT The objective of this research was to identify Mycobacterium bovis in lesions suggestive of tuberculosis in bovine carcasses in the State of Ceará, by means of bacteriological and molecular diagnostic tests. Between August 2017 and January 2019, the State inspection service (SIE) inspected 59,512 cattle, of which 7.4% (44 / 59,512) presented suggestive lesions. Of these animals, 68 samples were sent, of which 4.5% (31/68) located in the lung, 2.9% (20/68) in lymph nodes, 2.0% (14/68) in the liver, and 0.4% in the carcass (3/68). When performing bacteriological isolation, 15.9% (7/44) of bovines showed colony growth in the samples. The smears of the isolates were submitted to Zielh-Neelsen staining and all confirmed acid-fast bacilli. The polymerase chain reaction identified all isolates 100% (7/7) as M. bovis. The association of diagnostic techniques allowed to identify the presence of the agent in the State and the molecular analysis proved to be a beneficial technique in the monitoring of bovine tuberculosis and can be used as an auxiliary method in the bovine tuberculosis control and eradication program in the State of Ceará. |
| first_indexed | 2024-04-12T10:54:24Z |
| format | Article |
| id | doaj.art-913b69be73294c1c9c9216269b26d605 |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-04-12T10:54:24Z |
| publishDate | 2022-05-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-913b69be73294c1c9c9216269b26d6052022-12-22T03:36:07ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622022-05-0174233834410.1590/1678-4162-12394Isolation and molecular identification of Mycobacterium bovis in tissue lesions of cattle slaughtered in slaughterhouses located in the State of CearáJ.Q. Amorimhttps://orcid.org/0000-0002-6439-813XB.R. Santoshttps://orcid.org/0000-0001-6010-6876H.F. Fehlberghttps://orcid.org/0000-0003-0547-6182A.V. Silvahttps://orcid.org/0000-0002-9124-2747F.F. Ferreirahttps://orcid.org/0000-0002-4814-3401J.N. Costahttps://orcid.org/0000-0002-2371-0684F. Alzamora Filhohttps://orcid.org/0000-0001-6456-8998ABSTRACT The objective of this research was to identify Mycobacterium bovis in lesions suggestive of tuberculosis in bovine carcasses in the State of Ceará, by means of bacteriological and molecular diagnostic tests. Between August 2017 and January 2019, the State inspection service (SIE) inspected 59,512 cattle, of which 7.4% (44 / 59,512) presented suggestive lesions. Of these animals, 68 samples were sent, of which 4.5% (31/68) located in the lung, 2.9% (20/68) in lymph nodes, 2.0% (14/68) in the liver, and 0.4% in the carcass (3/68). When performing bacteriological isolation, 15.9% (7/44) of bovines showed colony growth in the samples. The smears of the isolates were submitted to Zielh-Neelsen staining and all confirmed acid-fast bacilli. The polymerase chain reaction identified all isolates 100% (7/7) as M. bovis. The association of diagnostic techniques allowed to identify the presence of the agent in the State and the molecular analysis proved to be a beneficial technique in the monitoring of bovine tuberculosis and can be used as an auxiliary method in the bovine tuberculosis control and eradication program in the State of Ceará.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000200338&lng=en&tlng=endiagnosismultiplex PCRMycobacterium sspepidemiology |
| spellingShingle | J.Q. Amorim B.R. Santos H.F. Fehlberg A.V. Silva F.F. Ferreira J.N. Costa F. Alzamora Filho Isolation and molecular identification of Mycobacterium bovis in tissue lesions of cattle slaughtered in slaughterhouses located in the State of Ceará Arquivo Brasileiro de Medicina Veterinária e Zootecnia diagnosis multiplex PCR Mycobacterium ssp epidemiology |
| title | Isolation and molecular identification of Mycobacterium bovis in tissue lesions of cattle slaughtered in slaughterhouses located in the State of Ceará |
| title_full | Isolation and molecular identification of Mycobacterium bovis in tissue lesions of cattle slaughtered in slaughterhouses located in the State of Ceará |
| title_fullStr | Isolation and molecular identification of Mycobacterium bovis in tissue lesions of cattle slaughtered in slaughterhouses located in the State of Ceará |
| title_full_unstemmed | Isolation and molecular identification of Mycobacterium bovis in tissue lesions of cattle slaughtered in slaughterhouses located in the State of Ceará |
| title_short | Isolation and molecular identification of Mycobacterium bovis in tissue lesions of cattle slaughtered in slaughterhouses located in the State of Ceará |
| title_sort | isolation and molecular identification of mycobacterium bovis in tissue lesions of cattle slaughtered in slaughterhouses located in the state of ceara |
| topic | diagnosis multiplex PCR Mycobacterium ssp epidemiology |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000200338&lng=en&tlng=en |
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