Phytochemical andrographolide modulates NF-κB and JNK in human neuroblastoma SH-SY5Y cells, a cell model for Parkinson's disease
Andrographis paniculata has been an important plant for traditional medicine in Asia for centuries. Andrographolide is the primary bioactive phytochemical from the plant and is known to exhibit many different protective effects through modulation of various proteins and signaling pathways. Andrograp...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020309658 |
| _version_ | 1811289761880473600 |
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| author | Albert J. Ketterman Jeerang Wongtrakul Chonticha Saisawang |
| author_facet | Albert J. Ketterman Jeerang Wongtrakul Chonticha Saisawang |
| author_sort | Albert J. Ketterman |
| collection | DOAJ |
| description | Andrographis paniculata has been an important plant for traditional medicine in Asia for centuries. Andrographolide is the primary bioactive phytochemical from the plant and is known to exhibit many different protective effects through modulation of various proteins and signaling pathways. Andrographolide has been reported to exert anti-inflammatory and neuroprotective effects as well as being an antioxidant itself. We therefore studied whether andrographolide could provide protective effects to the SH-SY5Y neuroblastoma cell model for Parkinson's disease. In this study, we observed andrographolide inhibiting activation of NF-κB p65 (nuclear factor kappa-light-chain-enhancer of activated B cells) and JNK MAPK (c-Jun N-terminal Kinase Mitogen-Activated Protein Kinase) pathways, however, it did not provide any protective effect against induced stress in the SH-SY5Y cells. We propose the sustained low-level activation of JNK and the inhibition of NF-κB promoted ROS (Reactive Oxygen Species) production that yielded the observed cell death. Therefore, the protective effects observed with andrographolide appear to be cell/tissue specific responses. |
| first_indexed | 2024-04-13T04:00:11Z |
| format | Article |
| id | doaj.art-913d13f9dd1944c2a09f91fe76d376dc |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-13T04:00:11Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-913d13f9dd1944c2a09f91fe76d376dc2022-12-22T03:03:30ZengElsevierHeliyon2405-84402020-06-0166e04121Phytochemical andrographolide modulates NF-κB and JNK in human neuroblastoma SH-SY5Y cells, a cell model for Parkinson's diseaseAlbert J. Ketterman0Jeerang Wongtrakul1Chonticha Saisawang2Institute of Molecular Biosciences, Mahidol University, 25/25 Putthamonthol Road 4, Salaya, Nakhon Pathom, 73170, ThailandCenter for Molecular and Cell Biology for Infectious Diseases, Research Institute for Health Sciences, Chiang Mai University, PO.BOX 80 CMU, Chiang Mai, 50200, ThailandInstitute of Molecular Biosciences, Mahidol University, 25/25 Putthamonthol Road 4, Salaya, Nakhon Pathom, 73170, Thailand; Corresponding author.Andrographis paniculata has been an important plant for traditional medicine in Asia for centuries. Andrographolide is the primary bioactive phytochemical from the plant and is known to exhibit many different protective effects through modulation of various proteins and signaling pathways. Andrographolide has been reported to exert anti-inflammatory and neuroprotective effects as well as being an antioxidant itself. We therefore studied whether andrographolide could provide protective effects to the SH-SY5Y neuroblastoma cell model for Parkinson's disease. In this study, we observed andrographolide inhibiting activation of NF-κB p65 (nuclear factor kappa-light-chain-enhancer of activated B cells) and JNK MAPK (c-Jun N-terminal Kinase Mitogen-Activated Protein Kinase) pathways, however, it did not provide any protective effect against induced stress in the SH-SY5Y cells. We propose the sustained low-level activation of JNK and the inhibition of NF-κB promoted ROS (Reactive Oxygen Species) production that yielded the observed cell death. Therefore, the protective effects observed with andrographolide appear to be cell/tissue specific responses.http://www.sciencedirect.com/science/article/pii/S2405844020309658BiotechnologyProteinsBiochemistryMolecular biologyAgingAndrographolide |
| spellingShingle | Albert J. Ketterman Jeerang Wongtrakul Chonticha Saisawang Phytochemical andrographolide modulates NF-κB and JNK in human neuroblastoma SH-SY5Y cells, a cell model for Parkinson's disease Heliyon Biotechnology Proteins Biochemistry Molecular biology Aging Andrographolide |
| title | Phytochemical andrographolide modulates NF-κB and JNK in human neuroblastoma SH-SY5Y cells, a cell model for Parkinson's disease |
| title_full | Phytochemical andrographolide modulates NF-κB and JNK in human neuroblastoma SH-SY5Y cells, a cell model for Parkinson's disease |
| title_fullStr | Phytochemical andrographolide modulates NF-κB and JNK in human neuroblastoma SH-SY5Y cells, a cell model for Parkinson's disease |
| title_full_unstemmed | Phytochemical andrographolide modulates NF-κB and JNK in human neuroblastoma SH-SY5Y cells, a cell model for Parkinson's disease |
| title_short | Phytochemical andrographolide modulates NF-κB and JNK in human neuroblastoma SH-SY5Y cells, a cell model for Parkinson's disease |
| title_sort | phytochemical andrographolide modulates nf κb and jnk in human neuroblastoma sh sy5y cells a cell model for parkinson s disease |
| topic | Biotechnology Proteins Biochemistry Molecular biology Aging Andrographolide |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020309658 |
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