Deciphering the Calcium Code: A Review of Calcium Activity Analysis Methods Employed to Identify Meaningful Activity in Early Neural Development
The intracellular and intercellular flux of calcium ions represents an ancient and universal mode of signaling that regulates an extensive array of cellular processes. Evidence for the central role of calcium signaling includes various techniques that allow the visualization of calcium activity in l...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/14/1/138 |
| _version_ | 1797344496107651072 |
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| author | Sudip Paudel Michelle Yue Rithvik Nalamalapu Margaret S. Saha |
| author_facet | Sudip Paudel Michelle Yue Rithvik Nalamalapu Margaret S. Saha |
| author_sort | Sudip Paudel |
| collection | DOAJ |
| description | The intracellular and intercellular flux of calcium ions represents an ancient and universal mode of signaling that regulates an extensive array of cellular processes. Evidence for the central role of calcium signaling includes various techniques that allow the visualization of calcium activity in living cells. While extensively investigated in mature cells, calcium activity is equally important in developing cells, particularly the embryonic nervous system where it has been implicated in a wide variety array of determinative events. However, unlike in mature cells, where the calcium dynamics display regular, predictable patterns, calcium activity in developing systems is far more sporadic, irregular, and diverse. This renders the ability to assess calcium activity in a consistent manner extremely challenging, challenges reflected in the diversity of methods employed to analyze calcium activity in neural development. Here we review the wide array of calcium detection and analysis methods used across studies, limiting the extent to which they can be comparatively analyzed. The goal is to provide investigators not only with an overview of calcium activity analysis techniques currently available, but also to offer suggestions for future work and standardization to enable informative comparative evaluations of this fundamental and important process in neural development. |
| first_indexed | 2024-03-08T11:04:31Z |
| format | Article |
| id | doaj.art-913ea17099764855b1cc68bc70bba243 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-08T11:04:31Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-913ea17099764855b1cc68bc70bba2432024-01-26T15:22:35ZengMDPI AGBiomolecules2218-273X2024-01-0114113810.3390/biom14010138Deciphering the Calcium Code: A Review of Calcium Activity Analysis Methods Employed to Identify Meaningful Activity in Early Neural DevelopmentSudip Paudel0Michelle Yue1Rithvik Nalamalapu2Margaret S. Saha3Wyss Institute, Harvard University, Boston, MA 02215, USAWyss Institute, Harvard University, Boston, MA 02215, USASchool of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USABiology Department|William & Mary, Williamsburg, VA 23185, USAThe intracellular and intercellular flux of calcium ions represents an ancient and universal mode of signaling that regulates an extensive array of cellular processes. Evidence for the central role of calcium signaling includes various techniques that allow the visualization of calcium activity in living cells. While extensively investigated in mature cells, calcium activity is equally important in developing cells, particularly the embryonic nervous system where it has been implicated in a wide variety array of determinative events. However, unlike in mature cells, where the calcium dynamics display regular, predictable patterns, calcium activity in developing systems is far more sporadic, irregular, and diverse. This renders the ability to assess calcium activity in a consistent manner extremely challenging, challenges reflected in the diversity of methods employed to analyze calcium activity in neural development. Here we review the wide array of calcium detection and analysis methods used across studies, limiting the extent to which they can be comparatively analyzed. The goal is to provide investigators not only with an overview of calcium activity analysis techniques currently available, but also to offer suggestions for future work and standardization to enable informative comparative evaluations of this fundamental and important process in neural development.https://www.mdpi.com/2218-273X/14/1/138calciumembryoneuralspikesdevelopmentreview |
| spellingShingle | Sudip Paudel Michelle Yue Rithvik Nalamalapu Margaret S. Saha Deciphering the Calcium Code: A Review of Calcium Activity Analysis Methods Employed to Identify Meaningful Activity in Early Neural Development Biomolecules calcium embryo neural spikes development review |
| title | Deciphering the Calcium Code: A Review of Calcium Activity Analysis Methods Employed to Identify Meaningful Activity in Early Neural Development |
| title_full | Deciphering the Calcium Code: A Review of Calcium Activity Analysis Methods Employed to Identify Meaningful Activity in Early Neural Development |
| title_fullStr | Deciphering the Calcium Code: A Review of Calcium Activity Analysis Methods Employed to Identify Meaningful Activity in Early Neural Development |
| title_full_unstemmed | Deciphering the Calcium Code: A Review of Calcium Activity Analysis Methods Employed to Identify Meaningful Activity in Early Neural Development |
| title_short | Deciphering the Calcium Code: A Review of Calcium Activity Analysis Methods Employed to Identify Meaningful Activity in Early Neural Development |
| title_sort | deciphering the calcium code a review of calcium activity analysis methods employed to identify meaningful activity in early neural development |
| topic | calcium embryo neural spikes development review |
| url | https://www.mdpi.com/2218-273X/14/1/138 |
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