Primary Screening of Microorganisms against <i>Meloidogyne hapla</i> (Chitwood, 1949) under the Conditions of Laboratory and Vegetative Tests on Tomato
Highly adapted obligate endoparasites of the root system, root-knot nematodes (<i>Meloidogyne</i> spp.), cause great damage to agricultural crops. Our research is aimed at the assessment of nematicidal activity and effectiveness of antagonist fungal and bacterial strains against the most...
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MDPI AG
2023-09-01
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| author | Svetlana N. Nekoval Arina K. Churikova Maxim N. Chernyakovich Mikhail V. Pridannikov |
| author_facet | Svetlana N. Nekoval Arina K. Churikova Maxim N. Chernyakovich Mikhail V. Pridannikov |
| author_sort | Svetlana N. Nekoval |
| collection | DOAJ |
| description | Highly adapted obligate endoparasites of the root system, root-knot nematodes (<i>Meloidogyne</i> spp.), cause great damage to agricultural crops. Our research is aimed at the assessment of nematicidal activity and effectiveness of antagonist fungal and bacterial strains against the most common type of root-knot nematode in the south of Russia. By means of molecular genetic identification, it was found that in the south of Russia, the species <i>Meloidogyne hapla</i> Chitwood, 1949 and <i>Meloidogyne incognita</i> (Kofoid and White, 1919) Chitwood, 1949 cause galls on the roots of open-ground and greenhouse tomato. Screening of microbial agents against second-stage juvenile (J2) <i>M. hapla</i> was carried out in the laboratory. At the end of the experiment, two liquid fungal cultures of <i>Paecilomyces lilacinus</i> BK-6 and <i>Metarhizium anisopliae</i> BK-2 were isolated, the nematicidal activity of which reached 100.0 and 70.2%, and exceeded the values of the biological standard (Nemotafagin-Mikopro) by 38.4% and 8.8%. The highest biological efficacy was noted in the liquid cultures of <i>P. lilacinus</i> BK-6, <i>M. anisopliae</i> BK-2, and <i>Arthrobotrys conoides</i> BK-8 when introduced into the soil before planting tomato. The number of formed galls on the roots was lower in comparison with the control by 81.0%, 75.5%, and 74.4%. |
| first_indexed | 2024-03-10T22:12:32Z |
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| language | English |
| last_indexed | 2024-03-10T22:12:32Z |
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| series | Plants |
| spelling | doaj.art-9146d8fa277d40448ed65604f30c96802023-11-19T12:33:16ZengMDPI AGPlants2223-77472023-09-011218332310.3390/plants12183323Primary Screening of Microorganisms against <i>Meloidogyne hapla</i> (Chitwood, 1949) under the Conditions of Laboratory and Vegetative Tests on TomatoSvetlana N. Nekoval0Arina K. Churikova1Maxim N. Chernyakovich2Mikhail V. Pridannikov3Federal Research Center of Biological Plant Protection, p/o 39, 350039 Krasnodar, RussiaFederal Research Center of Biological Plant Protection, p/o 39, 350039 Krasnodar, RussiaFederal Research Center of Biological Plant Protection, p/o 39, 350039 Krasnodar, RussiaCenter of Parasitology “A.N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences”, 33 Leninsky Prospekt, 119071 Moscow, RussiaHighly adapted obligate endoparasites of the root system, root-knot nematodes (<i>Meloidogyne</i> spp.), cause great damage to agricultural crops. Our research is aimed at the assessment of nematicidal activity and effectiveness of antagonist fungal and bacterial strains against the most common type of root-knot nematode in the south of Russia. By means of molecular genetic identification, it was found that in the south of Russia, the species <i>Meloidogyne hapla</i> Chitwood, 1949 and <i>Meloidogyne incognita</i> (Kofoid and White, 1919) Chitwood, 1949 cause galls on the roots of open-ground and greenhouse tomato. Screening of microbial agents against second-stage juvenile (J2) <i>M. hapla</i> was carried out in the laboratory. At the end of the experiment, two liquid fungal cultures of <i>Paecilomyces lilacinus</i> BK-6 and <i>Metarhizium anisopliae</i> BK-2 were isolated, the nematicidal activity of which reached 100.0 and 70.2%, and exceeded the values of the biological standard (Nemotafagin-Mikopro) by 38.4% and 8.8%. The highest biological efficacy was noted in the liquid cultures of <i>P. lilacinus</i> BK-6, <i>M. anisopliae</i> BK-2, and <i>Arthrobotrys conoides</i> BK-8 when introduced into the soil before planting tomato. The number of formed galls on the roots was lower in comparison with the control by 81.0%, 75.5%, and 74.4%.https://www.mdpi.com/2223-7747/12/18/3323microorganismsroot-knot nematodes<i>Meloidogyne hapla</i>nematicidal activitygallstomato |
| spellingShingle | Svetlana N. Nekoval Arina K. Churikova Maxim N. Chernyakovich Mikhail V. Pridannikov Primary Screening of Microorganisms against <i>Meloidogyne hapla</i> (Chitwood, 1949) under the Conditions of Laboratory and Vegetative Tests on Tomato Plants microorganisms root-knot nematodes <i>Meloidogyne hapla</i> nematicidal activity galls tomato |
| title | Primary Screening of Microorganisms against <i>Meloidogyne hapla</i> (Chitwood, 1949) under the Conditions of Laboratory and Vegetative Tests on Tomato |
| title_full | Primary Screening of Microorganisms against <i>Meloidogyne hapla</i> (Chitwood, 1949) under the Conditions of Laboratory and Vegetative Tests on Tomato |
| title_fullStr | Primary Screening of Microorganisms against <i>Meloidogyne hapla</i> (Chitwood, 1949) under the Conditions of Laboratory and Vegetative Tests on Tomato |
| title_full_unstemmed | Primary Screening of Microorganisms against <i>Meloidogyne hapla</i> (Chitwood, 1949) under the Conditions of Laboratory and Vegetative Tests on Tomato |
| title_short | Primary Screening of Microorganisms against <i>Meloidogyne hapla</i> (Chitwood, 1949) under the Conditions of Laboratory and Vegetative Tests on Tomato |
| title_sort | primary screening of microorganisms against i meloidogyne hapla i chitwood 1949 under the conditions of laboratory and vegetative tests on tomato |
| topic | microorganisms root-knot nematodes <i>Meloidogyne hapla</i> nematicidal activity galls tomato |
| url | https://www.mdpi.com/2223-7747/12/18/3323 |
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