A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex
Summary: Here, we describe a protocol to simultaneously record and label single cortical neurons in vivo under local application of a chemical such as a receptor agonist. This protocol provides a useful tool to investigate how the chemical of interest affects the processing of sensory information by...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721000241 |
| _version_ | 1818336622046871552 |
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| author | Ehsan Kheradpezhouh Wricha Mishra Ehsan Arabzadeh |
| author_facet | Ehsan Kheradpezhouh Wricha Mishra Ehsan Arabzadeh |
| author_sort | Ehsan Kheradpezhouh |
| collection | DOAJ |
| description | Summary: Here, we describe a protocol to simultaneously record and label single cortical neurons in vivo under local application of a chemical such as a receptor agonist. This protocol provides a useful tool to investigate how the chemical of interest affects the processing of sensory information by cortical neurons. The juxtacellular labeling allows identification of the cell type and morphology of the recorded neurons. We draw examples to show pharmacological modulations in encoding of vibrotactile stimuli in the mouse primary somatosensory cortex.For complete details on the use and execution of this protocol, please refer to Kheradpezhouh et al. (2020). |
| first_indexed | 2024-12-13T14:42:15Z |
| format | Article |
| id | doaj.art-914c010bccb34a3aabf38d47ab523805 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-13T14:42:15Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-914c010bccb34a3aabf38d47ab5238052022-12-21T23:41:35ZengElsevierSTAR Protocols2666-16672021-03-0121100317A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortexEhsan Kheradpezhouh0Wricha Mishra1Ehsan Arabzadeh2Eccles Institute of Neuroscience, John Curtin School of Medical Research, The Australian National University, Acton, Canberra, ACT 2601, Australia; The Australian Research Council Centre of Excellence for Integrative Brain Function, ACT Node, Caneberra, ACT 2601, Australia; Corresponding authorEccles Institute of Neuroscience, John Curtin School of Medical Research, The Australian National University, Acton, Canberra, ACT 2601, Australia; The Australian Research Council Centre of Excellence for Integrative Brain Function, ACT Node, Caneberra, ACT 2601, AustraliaEccles Institute of Neuroscience, John Curtin School of Medical Research, The Australian National University, Acton, Canberra, ACT 2601, Australia; The Australian Research Council Centre of Excellence for Integrative Brain Function, ACT Node, Caneberra, ACT 2601, AustraliaSummary: Here, we describe a protocol to simultaneously record and label single cortical neurons in vivo under local application of a chemical such as a receptor agonist. This protocol provides a useful tool to investigate how the chemical of interest affects the processing of sensory information by cortical neurons. The juxtacellular labeling allows identification of the cell type and morphology of the recorded neurons. We draw examples to show pharmacological modulations in encoding of vibrotactile stimuli in the mouse primary somatosensory cortex.For complete details on the use and execution of this protocol, please refer to Kheradpezhouh et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721000241ChemistryAnalytical chemistryChemical composition analysis |
| spellingShingle | Ehsan Kheradpezhouh Wricha Mishra Ehsan Arabzadeh A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex STAR Protocols Chemistry Analytical chemistry Chemical composition analysis |
| title | A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex |
| title_full | A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex |
| title_fullStr | A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex |
| title_full_unstemmed | A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex |
| title_short | A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex |
| title_sort | protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex |
| topic | Chemistry Analytical chemistry Chemical composition analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721000241 |
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