Dynamics of the digestive acquisition of bacterial carriage and integron presence by French preterm newborns according to maternal colonization: The DAIR3N multicentric study
ObjectivesThe study aimed to describe the dynamics and risk factors of Gram-negative bacteria (GNB) acquisition in preterm infants.MethodsThis prospective multicenter French study included mothers hospitalized for preterm delivery and their newborns, followed until hospital discharge. Maternal feces...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1148319/full |
| _version_ | 1827991016501673984 |
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| author | Alice Patry Philippe Bothorel Anaïs Labrunie Laurent Renesme Philippe Lehours Melinda Benard Damien Dubois Laure Ponthier Sylvain Meyer Karine Norbert Laurent Villeneuve Philippe Jouvencel David Leysenne Delphine Chainier Sandrine Luce Carole Grélaud Marie-Cecile Ploy Antoine Bedu Fabien Garnier |
| author_facet | Alice Patry Philippe Bothorel Anaïs Labrunie Laurent Renesme Philippe Lehours Melinda Benard Damien Dubois Laure Ponthier Sylvain Meyer Karine Norbert Laurent Villeneuve Philippe Jouvencel David Leysenne Delphine Chainier Sandrine Luce Carole Grélaud Marie-Cecile Ploy Antoine Bedu Fabien Garnier |
| author_sort | Alice Patry |
| collection | DOAJ |
| description | ObjectivesThe study aimed to describe the dynamics and risk factors of Gram-negative bacteria (GNB) acquisition in preterm infants.MethodsThis prospective multicenter French study included mothers hospitalized for preterm delivery and their newborns, followed until hospital discharge. Maternal feces and vaginal fluids at delivery, and neonatal feces from birth to discharge were tested for cultivable GNB, potential acquired resistance, and integrons. The primary outcome was the acquisition of GNB and integrons in neonatal feces, and their dynamics, evaluated by survival analysis using the actuarial method. Risk factors were analyzed using Cox models.ResultsTwo hundred thirty-eight evaluable preterm dyads were included by five different centers over 16 months. GNB were isolated in 32.6% of vaginal samples, with 15.4% of strains producing extended-spectrum beta-lactamase (ESBL) or hyperproducing cephalosporinase (HCase), and in 96.2% of maternal feces, with 7.8% ESBL-GNB or HCase-GNB. Integrons were detected in 40.2% of feces and 10.6% of GNB strains. The mean (SD) length of stay of newborns was 39.5 (15.9) days; 4 died in the hospital. At least one infection episode occurred in 36.1% of newborns. The acquisition of GNB and integrons was progressive from birth to discharge. At discharge, half of newborns had ESBL-GNB or HCase-GNB, independently favored by a premature rupture of membranes (Hazard Ratio (HR), 3.41, 95% confidence interval (CI), 1.71; 6.81), and 25.6% had integrons (protective factor: multiple gestation, HR, 0.367, 95% CI, 0.195; 0.693).ConclusionIn preterm newborns, the acquisitions of GNB, including resistant ones, and integrons are progressive from birth to discharge. A premature rupture of membranes favored the colonization by ESBL-GNB or Hcase-GNB. |
| first_indexed | 2024-04-10T00:44:07Z |
| format | Article |
| id | doaj.art-914c73cac629496280b0d6e4d52aa9dd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T00:44:07Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-914c73cac629496280b0d6e4d52aa9dd2023-03-14T04:28:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.11483191148319Dynamics of the digestive acquisition of bacterial carriage and integron presence by French preterm newborns according to maternal colonization: The DAIR3N multicentric studyAlice Patry0Philippe Bothorel1Anaïs Labrunie2Laurent Renesme3Philippe Lehours4Melinda Benard5Damien Dubois6Laure Ponthier7Sylvain Meyer8Karine Norbert9Laurent Villeneuve10Philippe Jouvencel11David Leysenne12Delphine Chainier13Sandrine Luce14Carole Grélaud15Marie-Cecile Ploy16Antoine Bedu17Fabien Garnier18INSERM UMR, Limoges University, Limoges University Hospital, Limoges, FranceDepartment of Pediatrics, Mother-Child Hospital, Limoges University Hospital, Limoges, FranceEpidemiology, Biostatistics, and Research Methodology Centre (CEBIMER), Limoges University Hospital, Limoges, FranceDepartment of Pediatrics, Neonatology and Maternity Unit, Pellegrin University Hospital, Bordeaux, FranceBacteriology Laboratory, Pellegrin University Hospital, Bordeaux, FranceDepartment of Pediatrics and Neonatology, CHU Toulouse, Toulouse, FranceBacteriology and Hygiene Department, Federative Institute of Biology, CHU Toulouse University Hospital, Toulouse, FranceDepartment of Pediatrics, Mother-Child Hospital, Limoges University Hospital, Limoges, FranceINSERM UMR, Limoges University, Limoges University Hospital, Limoges, FranceDepartment of Pediatrics, Pau Hospital, Pau, FranceMedical Biology Laboratory, Pau Hospital, Pau, France0Department of Pediatrics and Neonatology, « Côte Basque » Hospital, Bayonne, France1Microbiology Laboratory, « Côte Basque » Hospital, Bayonne, FranceINSERM UMR, Limoges University, Limoges University Hospital, Limoges, FranceEpidemiology, Biostatistics, and Research Methodology Centre (CEBIMER), Limoges University Hospital, Limoges, FranceINSERM UMR, Limoges University, Limoges University Hospital, Limoges, FranceINSERM UMR, Limoges University, Limoges University Hospital, Limoges, FranceDepartment of Pediatrics, Mother-Child Hospital, Limoges University Hospital, Limoges, FranceINSERM UMR, Limoges University, Limoges University Hospital, Limoges, FranceObjectivesThe study aimed to describe the dynamics and risk factors of Gram-negative bacteria (GNB) acquisition in preterm infants.MethodsThis prospective multicenter French study included mothers hospitalized for preterm delivery and their newborns, followed until hospital discharge. Maternal feces and vaginal fluids at delivery, and neonatal feces from birth to discharge were tested for cultivable GNB, potential acquired resistance, and integrons. The primary outcome was the acquisition of GNB and integrons in neonatal feces, and their dynamics, evaluated by survival analysis using the actuarial method. Risk factors were analyzed using Cox models.ResultsTwo hundred thirty-eight evaluable preterm dyads were included by five different centers over 16 months. GNB were isolated in 32.6% of vaginal samples, with 15.4% of strains producing extended-spectrum beta-lactamase (ESBL) or hyperproducing cephalosporinase (HCase), and in 96.2% of maternal feces, with 7.8% ESBL-GNB or HCase-GNB. Integrons were detected in 40.2% of feces and 10.6% of GNB strains. The mean (SD) length of stay of newborns was 39.5 (15.9) days; 4 died in the hospital. At least one infection episode occurred in 36.1% of newborns. The acquisition of GNB and integrons was progressive from birth to discharge. At discharge, half of newborns had ESBL-GNB or HCase-GNB, independently favored by a premature rupture of membranes (Hazard Ratio (HR), 3.41, 95% confidence interval (CI), 1.71; 6.81), and 25.6% had integrons (protective factor: multiple gestation, HR, 0.367, 95% CI, 0.195; 0.693).ConclusionIn preterm newborns, the acquisitions of GNB, including resistant ones, and integrons are progressive from birth to discharge. A premature rupture of membranes favored the colonization by ESBL-GNB or Hcase-GNB.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1148319/fullIntegronsdigestive acquisitionantimicrobial resistancepreterm newborn infantresistance markersgram-negative bacteria |
| spellingShingle | Alice Patry Philippe Bothorel Anaïs Labrunie Laurent Renesme Philippe Lehours Melinda Benard Damien Dubois Laure Ponthier Sylvain Meyer Karine Norbert Laurent Villeneuve Philippe Jouvencel David Leysenne Delphine Chainier Sandrine Luce Carole Grélaud Marie-Cecile Ploy Antoine Bedu Fabien Garnier Dynamics of the digestive acquisition of bacterial carriage and integron presence by French preterm newborns according to maternal colonization: The DAIR3N multicentric study Frontiers in Microbiology Integrons digestive acquisition antimicrobial resistance preterm newborn infant resistance markers gram-negative bacteria |
| title | Dynamics of the digestive acquisition of bacterial carriage and integron presence by French preterm newborns according to maternal colonization: The DAIR3N multicentric study |
| title_full | Dynamics of the digestive acquisition of bacterial carriage and integron presence by French preterm newborns according to maternal colonization: The DAIR3N multicentric study |
| title_fullStr | Dynamics of the digestive acquisition of bacterial carriage and integron presence by French preterm newborns according to maternal colonization: The DAIR3N multicentric study |
| title_full_unstemmed | Dynamics of the digestive acquisition of bacterial carriage and integron presence by French preterm newborns according to maternal colonization: The DAIR3N multicentric study |
| title_short | Dynamics of the digestive acquisition of bacterial carriage and integron presence by French preterm newborns according to maternal colonization: The DAIR3N multicentric study |
| title_sort | dynamics of the digestive acquisition of bacterial carriage and integron presence by french preterm newborns according to maternal colonization the dair3n multicentric study |
| topic | Integrons digestive acquisition antimicrobial resistance preterm newborn infant resistance markers gram-negative bacteria |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1148319/full |
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