Investigating the short- and long-term effects of antibacterial agents using a real-time assay based on bioluminescent E. coli-lux
We have previously established that the E. coli-lux assessment is a convenient tool for rapid measurements of the kinetical features of short-term toxicity caused by various factors. In this study, kinetic measurements of seven specifically acting model antibacterials (i.e., polymyxin B, chloramphen...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020310768 |
| _version_ | 1818919664118398976 |
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| author | Eetu N. Suominen Tuula Putus Janne Atosuo |
| author_facet | Eetu N. Suominen Tuula Putus Janne Atosuo |
| author_sort | Eetu N. Suominen |
| collection | DOAJ |
| description | We have previously established that the E. coli-lux assessment is a convenient tool for rapid measurements of the kinetical features of short-term toxicity caused by various factors. In this study, kinetic measurements of seven specifically acting model antibacterials (i.e., polymyxin B, chloramphenicol, nalidixic acid, kanamycin, deoxynivalenol, erythromycin and tetracycline) and two metals (AgNO3 and CdCl2) against E. coli-lux through a bioluminescence- and optical density-based real-time assay that combined short- and long-term toxicity assessments were performed. Bacteria were exposed to antibacterials and the effects were reported as the half-maximum effective concentration (EC50) after 30 min and 10 h. Regarding the 10-hour endpoints, all reference compounds, except deoxynivalenol, showed dose-response inhibition in the studied concentration range. The analysis of chloramphenicol, kanamycin, erythromycin, tetracycline and nalidixic acid clearly revealed the limitations of short-term inhibition tests. No significant differences were observed between the results obtained from luminescence inhibition and growth inhibition assays. The kinetical data from measurements provide differentiation between bacteriostatic and bactericidal mechanisms of various types of antibacterial agents. The combined assessment of short- and long-term effects reduces the risk of the underestimation of toxicity due to an inaccurate endpoint selection. The cost-efficient and fully automated E. coli-lux assessment technique may offer possibilities for high-throughput screening procedures. |
| first_indexed | 2024-12-20T01:09:27Z |
| format | Article |
| id | doaj.art-914da5badd4849b4b7f60ca14b20e1ad |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-20T01:09:27Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-914da5badd4849b4b7f60ca14b20e1ad2022-12-21T19:58:44ZengElsevierHeliyon2405-84402020-07-0167e04232Investigating the short- and long-term effects of antibacterial agents using a real-time assay based on bioluminescent E. coli-luxEetu N. Suominen0Tuula Putus1Janne Atosuo2The Department of Biochemistry, Faculty of Science and Engineering, University of Turku, Finland; Department of Occupational and Environmental Health, Faculty of Medicine, University of Turku, Finland; Corresponding author.Department of Occupational and Environmental Health, Faculty of Medicine, University of Turku, FinlandThe Department of Biochemistry, Faculty of Science and Engineering, University of Turku, FinlandWe have previously established that the E. coli-lux assessment is a convenient tool for rapid measurements of the kinetical features of short-term toxicity caused by various factors. In this study, kinetic measurements of seven specifically acting model antibacterials (i.e., polymyxin B, chloramphenicol, nalidixic acid, kanamycin, deoxynivalenol, erythromycin and tetracycline) and two metals (AgNO3 and CdCl2) against E. coli-lux through a bioluminescence- and optical density-based real-time assay that combined short- and long-term toxicity assessments were performed. Bacteria were exposed to antibacterials and the effects were reported as the half-maximum effective concentration (EC50) after 30 min and 10 h. Regarding the 10-hour endpoints, all reference compounds, except deoxynivalenol, showed dose-response inhibition in the studied concentration range. The analysis of chloramphenicol, kanamycin, erythromycin, tetracycline and nalidixic acid clearly revealed the limitations of short-term inhibition tests. No significant differences were observed between the results obtained from luminescence inhibition and growth inhibition assays. The kinetical data from measurements provide differentiation between bacteriostatic and bactericidal mechanisms of various types of antibacterial agents. The combined assessment of short- and long-term effects reduces the risk of the underestimation of toxicity due to an inaccurate endpoint selection. The cost-efficient and fully automated E. coli-lux assessment technique may offer possibilities for high-throughput screening procedures.http://www.sciencedirect.com/science/article/pii/S2405844020310768MicrobiologyBacteriaCell deathCytotoxicityMicrobial biotechnologyToxicology |
| spellingShingle | Eetu N. Suominen Tuula Putus Janne Atosuo Investigating the short- and long-term effects of antibacterial agents using a real-time assay based on bioluminescent E. coli-lux Heliyon Microbiology Bacteria Cell death Cytotoxicity Microbial biotechnology Toxicology |
| title | Investigating the short- and long-term effects of antibacterial agents using a real-time assay based on bioluminescent E. coli-lux |
| title_full | Investigating the short- and long-term effects of antibacterial agents using a real-time assay based on bioluminescent E. coli-lux |
| title_fullStr | Investigating the short- and long-term effects of antibacterial agents using a real-time assay based on bioluminescent E. coli-lux |
| title_full_unstemmed | Investigating the short- and long-term effects of antibacterial agents using a real-time assay based on bioluminescent E. coli-lux |
| title_short | Investigating the short- and long-term effects of antibacterial agents using a real-time assay based on bioluminescent E. coli-lux |
| title_sort | investigating the short and long term effects of antibacterial agents using a real time assay based on bioluminescent e coli lux |
| topic | Microbiology Bacteria Cell death Cytotoxicity Microbial biotechnology Toxicology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020310768 |
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