Molecular detection and characterization of Cryptosporidium spp. in the sewage-contaminated rivers entering Bandar-e Anzali Lagoon in Guilan Province, Iran
Waterborne cryptosporidiosis in river water is considered to be an important source of Cryptosporidium oocysts in most developing countries across the world. In the present study, 20 water samples were collected from Zarjoob and Goharrood rivers contaminated with wastewater in the province of Guilan...
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| Format: | Article |
| Language: | English |
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Kurdistan University of Medical Sciences
2020-04-01
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| Series: | Journal of Advances in Environmental Health Research |
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| Online Access: | http://jaehr.muk.ac.ir/article_107651_1ce46fb3d3519e409264388fb7c711f4.pdf |
| _version_ | 1819148400589799424 |
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| author | Mohammad Reza Mahmoudi |
| author_facet | Mohammad Reza Mahmoudi |
| author_sort | Mohammad Reza Mahmoudi |
| collection | DOAJ |
| description | Waterborne cryptosporidiosis in river water is considered to be an important source of Cryptosporidium oocysts in most developing countries across the world. In the present study, 20 water samples were collected from Zarjoob and Goharrood rivers contaminated with wastewater in the province of Guilan, Iran. The samples were filtrated through a membrane filter (pore size: 1.2 μm), then the sucrose clarification and molecular genotyping methods were also carried out. In total, 12 out of 20 water samples were positive for Cryptosporidium species based on the 18S RNA-based polymerase chain reaction (PCR) and sequencing techniques. In addition, C. parvum, C. muris, C. homnis, and C. canis were detected in the water samples studied. According to the results, river water resources were polluted with the pathogenic species of Cryptosporidium with human and animal sources, which increased the potential risk of waterborne cryptosporidiosis. Moreover, the long-term use of sewage-contaminated river water to irrigate crops and agriculture farms could be a major threat to local residents. |
| first_indexed | 2024-12-22T13:45:07Z |
| format | Article |
| id | doaj.art-914f5ce13c2443e78b5bab6064acf694 |
| institution | Directory Open Access Journal |
| issn | 2345-3990 2345-3990 |
| language | English |
| last_indexed | 2024-12-22T13:45:07Z |
| publishDate | 2020-04-01 |
| publisher | Kurdistan University of Medical Sciences |
| record_format | Article |
| series | Journal of Advances in Environmental Health Research |
| spelling | doaj.art-914f5ce13c2443e78b5bab6064acf6942022-12-21T18:23:48ZengKurdistan University of Medical SciencesJournal of Advances in Environmental Health Research2345-39902345-39902020-04-0182959910.22102/jaehr.2020.203874.1145107651Molecular detection and characterization of Cryptosporidium spp. in the sewage-contaminated rivers entering Bandar-e Anzali Lagoon in Guilan Province, IranMohammad Reza Mahmoudi0Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, IranWaterborne cryptosporidiosis in river water is considered to be an important source of Cryptosporidium oocysts in most developing countries across the world. In the present study, 20 water samples were collected from Zarjoob and Goharrood rivers contaminated with wastewater in the province of Guilan, Iran. The samples were filtrated through a membrane filter (pore size: 1.2 μm), then the sucrose clarification and molecular genotyping methods were also carried out. In total, 12 out of 20 water samples were positive for Cryptosporidium species based on the 18S RNA-based polymerase chain reaction (PCR) and sequencing techniques. In addition, C. parvum, C. muris, C. homnis, and C. canis were detected in the water samples studied. According to the results, river water resources were polluted with the pathogenic species of Cryptosporidium with human and animal sources, which increased the potential risk of waterborne cryptosporidiosis. Moreover, the long-term use of sewage-contaminated river water to irrigate crops and agriculture farms could be a major threat to local residents.http://jaehr.muk.ac.ir/article_107651_1ce46fb3d3519e409264388fb7c711f4.pdfbacterial isolationwater resourcessewage-contaminated riversguilan province |
| spellingShingle | Mohammad Reza Mahmoudi Molecular detection and characterization of Cryptosporidium spp. in the sewage-contaminated rivers entering Bandar-e Anzali Lagoon in Guilan Province, Iran Journal of Advances in Environmental Health Research bacterial isolation water resources sewage-contaminated rivers guilan province |
| title | Molecular detection and characterization of Cryptosporidium spp. in the sewage-contaminated rivers entering Bandar-e Anzali Lagoon in Guilan Province, Iran |
| title_full | Molecular detection and characterization of Cryptosporidium spp. in the sewage-contaminated rivers entering Bandar-e Anzali Lagoon in Guilan Province, Iran |
| title_fullStr | Molecular detection and characterization of Cryptosporidium spp. in the sewage-contaminated rivers entering Bandar-e Anzali Lagoon in Guilan Province, Iran |
| title_full_unstemmed | Molecular detection and characterization of Cryptosporidium spp. in the sewage-contaminated rivers entering Bandar-e Anzali Lagoon in Guilan Province, Iran |
| title_short | Molecular detection and characterization of Cryptosporidium spp. in the sewage-contaminated rivers entering Bandar-e Anzali Lagoon in Guilan Province, Iran |
| title_sort | molecular detection and characterization of cryptosporidium spp in the sewage contaminated rivers entering bandar e anzali lagoon in guilan province iran |
| topic | bacterial isolation water resources sewage-contaminated rivers guilan province |
| url | http://jaehr.muk.ac.ir/article_107651_1ce46fb3d3519e409264388fb7c711f4.pdf |
| work_keys_str_mv | AT mohammadrezamahmoudi moleculardetectionandcharacterizationofcryptosporidiumsppinthesewagecontaminatedriversenteringbandareanzalilagooninguilanprovinceiran |