Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut
The maintenance of intestinal barrier function is essential for preventing different pathologies, such as the leaky gut syndrome (LGS), which is characterized by the passage of harmful agents, like bacteria, toxins, and viruses, into the bloodstream. Intestinal barrier integrity is controlled by sev...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1157164/full |
| _version_ | 1827986777063817216 |
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| author | Francesca Algieri Nina Tanaskovic Cindy Cardenas Rincon Elisabetta Notario Daniele Braga Graziano Pesole Graziano Pesole Roberto Rusconi Roberto Rusconi Giuseppe Penna Giuseppe Penna Maria Rescigno Maria Rescigno |
| author_facet | Francesca Algieri Nina Tanaskovic Cindy Cardenas Rincon Elisabetta Notario Daniele Braga Graziano Pesole Graziano Pesole Roberto Rusconi Roberto Rusconi Giuseppe Penna Giuseppe Penna Maria Rescigno Maria Rescigno |
| author_sort | Francesca Algieri |
| collection | DOAJ |
| description | The maintenance of intestinal barrier function is essential for preventing different pathologies, such as the leaky gut syndrome (LGS), which is characterized by the passage of harmful agents, like bacteria, toxins, and viruses, into the bloodstream. Intestinal barrier integrity is controlled by several players, including the gut microbiota. Various molecules, called postbiotics, are released during the natural metabolic activity of the microbiota. Postbiotics can regulate host–microbe interactions, epithelial homeostasis, and have overall benefits for our health. In this work, we used in vitro and in vivo systems to demonstrate the role of Lactobacillus paracasei CNCM I-5220-derived postbiotic (LP-PBF) in preserving intestinal barrier integrity. We demonstrated in vitro that LP-PBF restored the morphology of tight junctions (TJs) that were altered upon Salmonella typhimurium exposure. In vivo, LP-PBF protected the gut vascular barrier and blocked S. typhimurium dissemination into the bloodstream. Interestingly, we found that LP-PBF interacts not only with the host cells, but also directly with S. typhimurium blocking its biofilm formation, partially due to the presence of biosurfactants. This study highlights that LP-PBF is beneficial in maintaining gut homeostasis due to the synergistic effect of its different components. These results suggest that LP-PBF could be utilized in managing several pathologies displaying an impaired intestinal barrier function. |
| first_indexed | 2024-04-09T23:36:22Z |
| format | Article |
| id | doaj.art-915070fe6c394d2ebb1aa37e6964a92e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T23:36:22Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-915070fe6c394d2ebb1aa37e6964a92e2023-03-20T05:51:49ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.11571641157164Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gutFrancesca Algieri0Nina Tanaskovic1Cindy Cardenas Rincon2Elisabetta Notario3Daniele Braga4Graziano Pesole5Graziano Pesole6Roberto Rusconi7Roberto Rusconi8Giuseppe Penna9Giuseppe Penna10Maria Rescigno11Maria Rescigno12Postbiotica S.r.l., Milan, ItalyPostbiotica S.r.l., Milan, ItalyIRCCS Humanitas Research Hospital, Rozzano, ItalyDepartment of Bioscience, Biotechnologies and Environment – DBBA, University of Bari Aldo Moro, Bari, ItalyIRCCS Humanitas Research Hospital, Rozzano, ItalyDepartment of Bioscience, Biotechnologies and Environment – DBBA, University of Bari Aldo Moro, Bari, ItalyInstitute of Biomembranes, Bioenergetics and Molecular Biotechnologies, Consiglio Nazionale delle Ricerche, Bari, ItalyIRCCS Humanitas Research Hospital, Rozzano, ItalyDepartment of Biomedical Sciences, Humanitas University, Pieve Emanuele, ItalyPostbiotica S.r.l., Milan, ItalyIRCCS Humanitas Research Hospital, Rozzano, ItalyIRCCS Humanitas Research Hospital, Rozzano, ItalyDepartment of Biomedical Sciences, Humanitas University, Pieve Emanuele, ItalyThe maintenance of intestinal barrier function is essential for preventing different pathologies, such as the leaky gut syndrome (LGS), which is characterized by the passage of harmful agents, like bacteria, toxins, and viruses, into the bloodstream. Intestinal barrier integrity is controlled by several players, including the gut microbiota. Various molecules, called postbiotics, are released during the natural metabolic activity of the microbiota. Postbiotics can regulate host–microbe interactions, epithelial homeostasis, and have overall benefits for our health. In this work, we used in vitro and in vivo systems to demonstrate the role of Lactobacillus paracasei CNCM I-5220-derived postbiotic (LP-PBF) in preserving intestinal barrier integrity. We demonstrated in vitro that LP-PBF restored the morphology of tight junctions (TJs) that were altered upon Salmonella typhimurium exposure. In vivo, LP-PBF protected the gut vascular barrier and blocked S. typhimurium dissemination into the bloodstream. Interestingly, we found that LP-PBF interacts not only with the host cells, but also directly with S. typhimurium blocking its biofilm formation, partially due to the presence of biosurfactants. This study highlights that LP-PBF is beneficial in maintaining gut homeostasis due to the synergistic effect of its different components. These results suggest that LP-PBF could be utilized in managing several pathologies displaying an impaired intestinal barrier function.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1157164/fullpostbioticleaky-gutLactobacillus paracaseitight junctionepithelial barrier integritymicrobiota |
| spellingShingle | Francesca Algieri Nina Tanaskovic Cindy Cardenas Rincon Elisabetta Notario Daniele Braga Graziano Pesole Graziano Pesole Roberto Rusconi Roberto Rusconi Giuseppe Penna Giuseppe Penna Maria Rescigno Maria Rescigno Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut Frontiers in Microbiology postbiotic leaky-gut Lactobacillus paracasei tight junction epithelial barrier integrity microbiota |
| title | Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut |
| title_full | Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut |
| title_fullStr | Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut |
| title_full_unstemmed | Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut |
| title_short | Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut |
| title_sort | lactobacillus paracasei cncm i 5220 derived postbiotic protects from the leaky gut |
| topic | postbiotic leaky-gut Lactobacillus paracasei tight junction epithelial barrier integrity microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1157164/full |
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