Cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitis
Background. There are no complete data on the occurrence of bacteria in the outbreak of chronic inflammation a consequence of their high virulence, or the result of a defect of local mechanisms of immune protection. To answer these questions, as well as to evaluate the nature, severity changes peria...
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| Format: | Article |
| Language: | Russian |
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Ministry of Health of Ukraine. Dnipropetrovsk Medical Academy
2014-06-01
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| Series: | Морфологія |
| Subjects: | |
| Online Access: | http://www.morphology.dp.ua/_pub/MORPHO-2014-08-02/14gpivhp.pdf |
| _version_ | 1831680045311066112 |
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| author | Gritsenko P.I. Petruk N.S. Samoylenko A.V. Tverdokhleb I.V. |
| author_facet | Gritsenko P.I. Petruk N.S. Samoylenko A.V. Tverdokhleb I.V. |
| author_sort | Gritsenko P.I. |
| collection | DOAJ |
| description | Background. There are no complete data on the occurrence of bacteria in the outbreak of chronic inflammation a consequence of their high virulence, or the result of a defect of local mechanisms of immune protection. To answer these questions, as well as to evaluate the nature, severity changes periapical tissues of the tooth and the activity of the inflammatory process in the apex of the tooth root is possible only during the morphological study of biopsy material. Objective. The aim of this work was to study the cellular composition and ultrastructural features of the granulation tissue formed during the current the primary and secondary apical periodontitis. Methods. There were included in the histological examination of the teeth 78, who underwent resection of the apex, followed by a fence of histological material. For ultrastructural studies of granuloma periodontal tissue samples were fixed with 2.5% glutaraldehyde solution, material embedded in Epon-812 («SPI-Pon ™ 812 Epoxy Embedding Kit», USA). The study was conducted using a transmission electron microscopy. Results. In the development of destructive periodontal inflammation are actively involved both specific and non-specific mechanisms of immunity. Conclusion. In primary periodontitis granulation tissue contains a moderate amount of white blood cells of various types, as well as single macrophages and plasma cells with reduced functional activity. In secondary periodontitis granuloma contains numerous mononuclear macrophages, plasma cells and giant multinucleated cells with increased activity, which corresponds to the morphological picture of the formation of immune granulomas.
Citation: Gritsenko PI, Petruk NS, Samoylenko AV, Tverdokhleb IV. [Cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitis]. Morphologia. 2014;8(2):14-9. Russian. |
| first_indexed | 2024-12-20T05:30:33Z |
| format | Article |
| id | doaj.art-91520a0ff5a143c5bae7da658f5ccaba |
| institution | Directory Open Access Journal |
| issn | 1997-9665 1997-9665 |
| language | Russian |
| last_indexed | 2024-12-20T05:30:33Z |
| publishDate | 2014-06-01 |
| publisher | Ministry of Health of Ukraine. Dnipropetrovsk Medical Academy |
| record_format | Article |
| series | Морфологія |
| spelling | doaj.art-91520a0ff5a143c5bae7da658f5ccaba2022-12-21T19:51:45ZrusMinistry of Health of Ukraine. Dnipropetrovsk Medical AcademyМорфологія1997-96651997-96652014-06-01821419Cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitisGritsenko P.I.0Petruk N.S.1Samoylenko A.V.2Tverdokhleb I.V.3State establishment “Dnipropetrovsk Medical Academy of Health Ministry of Ukraine”State establishment “Dnipropetrovsk Medical Academy of Health Ministry of Ukraine”State establishment “Dnipropetrovsk Medical Academy of Health Ministry of Ukraine”State establishment “Dnipropetrovsk Medical Academy of Health Ministry of Ukraine”Background. There are no complete data on the occurrence of bacteria in the outbreak of chronic inflammation a consequence of their high virulence, or the result of a defect of local mechanisms of immune protection. To answer these questions, as well as to evaluate the nature, severity changes periapical tissues of the tooth and the activity of the inflammatory process in the apex of the tooth root is possible only during the morphological study of biopsy material. Objective. The aim of this work was to study the cellular composition and ultrastructural features of the granulation tissue formed during the current the primary and secondary apical periodontitis. Methods. There were included in the histological examination of the teeth 78, who underwent resection of the apex, followed by a fence of histological material. For ultrastructural studies of granuloma periodontal tissue samples were fixed with 2.5% glutaraldehyde solution, material embedded in Epon-812 («SPI-Pon ™ 812 Epoxy Embedding Kit», USA). The study was conducted using a transmission electron microscopy. Results. In the development of destructive periodontal inflammation are actively involved both specific and non-specific mechanisms of immunity. Conclusion. In primary periodontitis granulation tissue contains a moderate amount of white blood cells of various types, as well as single macrophages and plasma cells with reduced functional activity. In secondary periodontitis granuloma contains numerous mononuclear macrophages, plasma cells and giant multinucleated cells with increased activity, which corresponds to the morphological picture of the formation of immune granulomas. Citation: Gritsenko PI, Petruk NS, Samoylenko AV, Tverdokhleb IV. [Cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitis]. Morphologia. 2014;8(2):14-9. Russian.http://www.morphology.dp.ua/_pub/MORPHO-2014-08-02/14gpivhp.pdfprimary chronic periodontitissecondary chronic periodontitishistological structureultrastructure |
| spellingShingle | Gritsenko P.I. Petruk N.S. Samoylenko A.V. Tverdokhleb I.V. Cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitis Морфологія primary chronic periodontitis secondary chronic periodontitis histological structure ultrastructure |
| title | Cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitis |
| title_full | Cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitis |
| title_fullStr | Cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitis |
| title_full_unstemmed | Cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitis |
| title_short | Cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitis |
| title_sort | cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitis |
| topic | primary chronic periodontitis secondary chronic periodontitis histological structure ultrastructure |
| url | http://www.morphology.dp.ua/_pub/MORPHO-2014-08-02/14gpivhp.pdf |
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