Curcumin Alleviates the Senescence of Canine Bone Marrow Mesenchymal Stem Cells during In Vitro Expansion by Activating the Autophagy Pathway
Senescence in mesenchymal stem cells (MSCs) not only hinders the application of MSCs in regenerative medicine but is also closely correlated with biological aging and the development of degenerative diseases. In this study, we investigated the anti-aging effects of curcumin (Cur) on canine bone marr...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/21/11356 |
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| author | Jiaqiang Deng Ping Ouyang Weiyao Li Lijun Zhong Congwei Gu Liuhong Shen Suizhong Cao Lizi Yin Zhihua Ren Zhicai Zuo Junliang Deng Qigui Yan Shumin Yu |
| author_facet | Jiaqiang Deng Ping Ouyang Weiyao Li Lijun Zhong Congwei Gu Liuhong Shen Suizhong Cao Lizi Yin Zhihua Ren Zhicai Zuo Junliang Deng Qigui Yan Shumin Yu |
| author_sort | Jiaqiang Deng |
| collection | DOAJ |
| description | Senescence in mesenchymal stem cells (MSCs) not only hinders the application of MSCs in regenerative medicine but is also closely correlated with biological aging and the development of degenerative diseases. In this study, we investigated the anti-aging effects of curcumin (Cur) on canine bone marrow-derived MSCs (cBMSCs), and further elucidated the potential mechanism of action based on the modulation of autophagy. cBMSCs were expanded in vitro with standard procedures to construct a cell model of premature senescence. Our evidence indicates that compared with the third passage of cBMSCs, many typical senescence-associated phenotypes were observed in the sixth passage of cBMSCs. Cur treatment can improve cBMSC survival and retard cBMSC senescence according to observations that Cur (1 μM) treatment can improve the colony-forming unit-fibroblasts (CFU-Fs) efficiency and upregulated the mRNA expression of pluripotent transcription factors (SOX-2 and Nanog), as well as inhibiting the senescence-associated beta-galactosidase (SA-β-gal) activities and mRNA expression of the senescence-related markers (p16 and p21) and pro-inflammatory molecules (tumor necrosis factor-α (<i>TNF-α</i>) and interleukin-6 (IL-6)). Furthermore, Cur (0.1 μM~10 μM) was observed to increase autophagic activity, as identified by upregulation of microtubule-associated protein 1 light chain 3 (LC3), unc51-like autophagy-activating kinase-1 (ULK1), autophagy-related gene<i> (</i>Atg)<i> 7</i> and Atg12, and the generation of type II of light chain 3 (LC3-II), thereby increasing autophagic vacuoles and acidic vesicular organelles, as well as causing a significant decrease in the p62 protein level. Moreover, the autophagy activator rapamycin (RAP) and Cur were found to partially ameliorate the senescent features of cBMSCs, while the autophagy inhibitor 3-methyladenine (3-MA) was shown to aggravate cBMSCs senescence and Cur treatment was able to restore the suppressed autophagy and counteract 3-MA-induced cBMSC senescence. Hence, our study highlights the important role of Cur-induced autophagy and its effects for ameliorating cBMSC senescence and provides new insight for delaying senescence and improving the therapeutic potential of MSCs. |
| first_indexed | 2024-03-10T06:02:49Z |
| format | Article |
| id | doaj.art-9154dfe513324bab97a9468703a40f05 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:02:49Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9154dfe513324bab97a9468703a40f052023-11-22T20:50:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122211135610.3390/ijms222111356Curcumin Alleviates the Senescence of Canine Bone Marrow Mesenchymal Stem Cells during In Vitro Expansion by Activating the Autophagy PathwayJiaqiang Deng0Ping Ouyang1Weiyao Li2Lijun Zhong3Congwei Gu4Liuhong Shen5Suizhong Cao6Lizi Yin7Zhihua Ren8Zhicai Zuo9Junliang Deng10Qigui Yan11Shumin Yu12College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaSenescence in mesenchymal stem cells (MSCs) not only hinders the application of MSCs in regenerative medicine but is also closely correlated with biological aging and the development of degenerative diseases. In this study, we investigated the anti-aging effects of curcumin (Cur) on canine bone marrow-derived MSCs (cBMSCs), and further elucidated the potential mechanism of action based on the modulation of autophagy. cBMSCs were expanded in vitro with standard procedures to construct a cell model of premature senescence. Our evidence indicates that compared with the third passage of cBMSCs, many typical senescence-associated phenotypes were observed in the sixth passage of cBMSCs. Cur treatment can improve cBMSC survival and retard cBMSC senescence according to observations that Cur (1 μM) treatment can improve the colony-forming unit-fibroblasts (CFU-Fs) efficiency and upregulated the mRNA expression of pluripotent transcription factors (SOX-2 and Nanog), as well as inhibiting the senescence-associated beta-galactosidase (SA-β-gal) activities and mRNA expression of the senescence-related markers (p16 and p21) and pro-inflammatory molecules (tumor necrosis factor-α (<i>TNF-α</i>) and interleukin-6 (IL-6)). Furthermore, Cur (0.1 μM~10 μM) was observed to increase autophagic activity, as identified by upregulation of microtubule-associated protein 1 light chain 3 (LC3), unc51-like autophagy-activating kinase-1 (ULK1), autophagy-related gene<i> (</i>Atg)<i> 7</i> and Atg12, and the generation of type II of light chain 3 (LC3-II), thereby increasing autophagic vacuoles and acidic vesicular organelles, as well as causing a significant decrease in the p62 protein level. Moreover, the autophagy activator rapamycin (RAP) and Cur were found to partially ameliorate the senescent features of cBMSCs, while the autophagy inhibitor 3-methyladenine (3-MA) was shown to aggravate cBMSCs senescence and Cur treatment was able to restore the suppressed autophagy and counteract 3-MA-induced cBMSC senescence. Hence, our study highlights the important role of Cur-induced autophagy and its effects for ameliorating cBMSC senescence and provides new insight for delaying senescence and improving the therapeutic potential of MSCs.https://www.mdpi.com/1422-0067/22/21/11356curcuminsenescenceautophagycanine bone marrow-derived mesenchymal stem cells |
| spellingShingle | Jiaqiang Deng Ping Ouyang Weiyao Li Lijun Zhong Congwei Gu Liuhong Shen Suizhong Cao Lizi Yin Zhihua Ren Zhicai Zuo Junliang Deng Qigui Yan Shumin Yu Curcumin Alleviates the Senescence of Canine Bone Marrow Mesenchymal Stem Cells during In Vitro Expansion by Activating the Autophagy Pathway International Journal of Molecular Sciences curcumin senescence autophagy canine bone marrow-derived mesenchymal stem cells |
| title | Curcumin Alleviates the Senescence of Canine Bone Marrow Mesenchymal Stem Cells during In Vitro Expansion by Activating the Autophagy Pathway |
| title_full | Curcumin Alleviates the Senescence of Canine Bone Marrow Mesenchymal Stem Cells during In Vitro Expansion by Activating the Autophagy Pathway |
| title_fullStr | Curcumin Alleviates the Senescence of Canine Bone Marrow Mesenchymal Stem Cells during In Vitro Expansion by Activating the Autophagy Pathway |
| title_full_unstemmed | Curcumin Alleviates the Senescence of Canine Bone Marrow Mesenchymal Stem Cells during In Vitro Expansion by Activating the Autophagy Pathway |
| title_short | Curcumin Alleviates the Senescence of Canine Bone Marrow Mesenchymal Stem Cells during In Vitro Expansion by Activating the Autophagy Pathway |
| title_sort | curcumin alleviates the senescence of canine bone marrow mesenchymal stem cells during in vitro expansion by activating the autophagy pathway |
| topic | curcumin senescence autophagy canine bone marrow-derived mesenchymal stem cells |
| url | https://www.mdpi.com/1422-0067/22/21/11356 |
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